Tmub2 (NM_001302505) Mouse Tagged ORF Clone

CAT#: MR229222

Tmub2 (myc-DDK-tagged) - Mouse transmembrane and ubiquitin-like domain containing 2 (Tmub2), transcript variant 1

Product Images

Circular map for MR229222

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmub2
• Synonyms: 2010008E23Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229222 representing NM_001302505
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTCACGTCTTCTTCAAAACAACCTTATGAGCGTGGACCCAGTCAGCAGCCAGGCCATGGAGCTCT
  CTGATGTCACCCTCATTGAGGGTGTGGGTAACGAGGTGATGGTGGTAGCAGGCGTGGTGGCGCTGACTCT
  AGCCCTGGTCCTAGCCTGGCTCTCCACCTATGTAGCAGACAGTGGTAACAACCAGCTGCTGGGCACCATT
  GTGTCAGCAGGTGACACGTCTGTTCTCCACCTGGGCCATGTGGACCAGCTGGTAAACCAAGGCACTCCAG
  AGCCAACCGAACACCCCCATCCATCAGGGGGCAATGATGACAAGGCTGAAGAGACCAGTGACAGTGGGGG
  AGACGCCACTGGAGAACCTGGAGCTAGGGGAGAGATGGAGCCCAGCCTGGAGCATCTCCTGGACATCCAA
  GGCCTGCCTAAAAGGCAAGCAGGCCTGGGGAGCAGTCGCCCAGAAGCCCCGCTGGGGTTAGATGATGGCT
  CCTGCCTCTCTCCCAGCCCCAGCCTCATCAATGTTCGCCTCAAGTTCCTCAATGACACCGAGGAGCTAGC
  TGTGGCCAGGCCAGAGGACACTGTGGGTACCCTAAAAAGCAAATACTTCCCTGGACAAGAGAGCCAAATG
  AAGTTGATCTACCAGGGTCGGCTGCTGCAGGACCCAGCACGCACACTGAGTTCCCTGAACATTACCAACA
  ACTGCGTGATCCACTGCCACCGCTCACCCCCAGGGGCAGCAGTTTCTGGCCCCTCAGCCTCCTTGACCCC
  AACCACTGAGCAATCTAGCCTTGGTGTCAACGTGGGCAGCCTCATGGTGCCTGTGTTTGTGGTGCTCTTG
  GGTGTGGTCTGGTACTTCCGTATCAATTACCGCCAGTTCTTCACTGGACCTGCCACTATCTCCTTGGTTG
  GAGTCACAGTCTTCTTCAGCATCCTGGTATTTGGGATGTATGGAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229222 representing NM_001302505
  Red=Cloning site Green=Tags(s)
  
  MISRLLQNNLMSVDPVSSQAMELSDVTLIEGVGNEVMVVAGVVALTLALVLAWLSTYVADSGNNQLLGTI
  VSAGDTSVLHLGHVDQLVNQGTPEPTEHPHPSGGNDDKAEETSDSGGDATGEPGARGEMEPSLEHLLDIQ
  GLPKRQAGLGSSRPEAPLGLDDGSCLSPSPSLINVRLKFLNDTEELAVARPEDTVGTLKSKYFPGQESQM
  KLIYQGRLLQDPARTLSSLNITNNCVIHCHRSPPGAAVSGPSASLTPTTEQSSLGVNVGSLMVPVFVVLL
  GVVWYFRINYRQFFTGPATISLVGVTVFFSILVFGMYGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302505
• ORF Size: 957 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302505.2
• RefSeq Size: 2711 bp
• RefSeq ORF: 960 bp
• Locus ID: 72053
• UniProt ID: Q3V209
• Cytogenetics: 11 D
• MW: 33.8 kDa
• Gene Summary: This gene encodes a predicted multi-pass membrane protein that features a C-terminal ubiquitin-related domain. This gene may be expressed in cone photoreceptors in the retina. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]