Kcnj11 (NM_001204411) Mouse Tagged ORF Clone

CAT#: MR229118

Kcnj11 (myc-DDK-tagged) - Mouse potassium inwardly rectifying channel, subfamily J, member 11 (Kcnj11), transcript variant 2

Product Images

Circular map for MR229118

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnj11
• Synonyms: Kir6.2; mBIR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229118 representing NM_001204411
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTGGTGGCTCATCGCCTTCGCCCACGGTGACCTGGCCCCCGGAGAGGGCACCAATGTGCCCTGCG
  TCACAAGCATCCACTCCTTTTCATCTGCCTTCCTTTTCTCCATCGAGGTCCAGGTGACCATTGGTTTCGG
  CGGGCGCATGGTGACAGAGGAATGTCCCCTGGCCATCCTCATTCTCATTGTGCAGAATATCGTCGGGCTG
  ATGATCAACGCCATCATGCTGGGCTGCATCTTCATGAAAACGGCCCAGGCCCATCGGCGGGCAGAAACCC
  TCATCTTCAGCAAGCATGCTGTGATCACCCTGCGCCATGGCCGCCTGTGCTTCATGCTGCGCGTAGGGGA
  CCTCCGAAAGAGCATGATCATTAGCGCCACCATCCACATGCAGGTGGTGCGCAAGACCACCAGCCCCGAG
  GGCGAAGTTGTGCCTCTCCACCAGGTAGACATCCCCATGGAGAATGGCGTGGGTGGTAACGGCATCTTCC
  TGGTGGCCCCACTCATCATCTACCACGTCATCGACTCCAACAGCCCGCTCTACGACCTGGCTCCTAGTGA
  CCTGCACCACCACCAGGACCTGGAGATCATTGTCATCTTGGAAGGCGTGGTAGAAACCACGGGCATCACC
  ACCCAGGCCCGCACCTCCTACCTAGCTGACGAGATTCTATGGGGGCAGCGCTTTGTCCCCATTGTGGCCG
  AGGAGGACGGCCGCTATTCTGTGGACTACTCCAAATTTGGTAACACCATTAAAGTGCCCACACCACTCTG
  CACAGCCCGCCAGCTTGATGAGGACCGCAGTCTGCTGGATGCCCTGACCCTCGCCTCGTCGCGGGGGCCC
  CTGCGCAAGCGCAGTGTGGCTGTGGCGAAGGCCAAGCCCAAGTTTAGCATCTCTCCAGATTCCTTGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229118 representing NM_001204411
  Red=Cloning site Green=Tags(s)
  
  MVWWLIAFAHGDLAPGEGTNVPCVTSIHSFSSAFLFSIEVQVTIGFGGRMVTEECPLAILILIVQNIVGL
  MINAIMLGCIFMKTAQAHRRAETLIFSKHAVITLRHGRLCFMLRVGDLRKSMIISATIHMQVVRKTTSPE
  GEVVPLHQVDIPMENGVGGNGIFLVAPLIIYHVIDSNSPLYDLAPSDLHHHQDLEIIVILEGVVETTGIT
  TQARTSYLADEILWGQRFVPIVAEEDGRYSVDYSKFGNTIKVPTPLCTARQLDEDRSLLDALTLASSRGP
  LRKRSVAVAKAKPKFSISPDSLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001204411
• ORF Size: 909 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001204411.1, NP_001191340.1
• RefSeq Size: 2667 bp
• RefSeq ORF: 912 bp
• Locus ID: 16514
• Cytogenetics: 7 29.66 cM
• MW: 33.7 kDa
• Gene Summary: This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation (By similarity).[UniProtKB/Swiss-Prot Function]