Capzb (NM_001271405) Mouse Tagged ORF Clone

CAT#: MR229106

Capzb (myc-DDK-tagged) - Mouse capping protein (actin filament) muscle Z-line, beta (Capzb), transcript variant 3

Product Images

Circular map for MR229106

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Capzb
• Synonyms: 1700120C01Rik; AI325129; Cap; Cappb1; CPB; CPB1; CPB2; CPbeat2; CPbet; CPbeta1; CPbeta2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229106 representing NM_001271405
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCCTAGCAGGCGCAGCCTTCCTTTCCCCCTGAACTGTCAGCTTGTTAGAGTTGGGACTGCTGATT
  ATGGAGGTGCCTCGGAGCAGAGCGATCAGCAGCTGGACTGCGCCTTGGACCTGATGAGGCGCCTGCCTCC
  ACAGCAGATTGAGAAGAACCTCAGCGATCTGATCGACCTGGTCCCCAGTCTGTGTGAAGATCTCCTGTCA
  TCTGTTGACCAGCCCCTGAAAATTGCCAGAGACAAGGTGGTGGGCAAGGATTACCTTTTGTGTGACTACA
  ACAGAGACGGGGACTCCTATAGGTCACCGTGGAGTAACAAGTATGACCCTCCTTTGGAAGATGGGGCCAT
  GCCATCTGCTCGGCTCAGAAAGCTGGAGGTAGAGGCCAACAATGCCTTCGACCAATACCGAGACCTGTAT
  TTTGAAGGTGGGGTCTCATCAGTCTACCTCTGGGATCTTGATCATGGCTTTGCTGGAGTGATCCTCATAA
  AGAAAGCTGGAGATGGATCCAAGAAGATCAAAGGCTGCTGGGATTCCATCCACGTGGTGGAAGTGCAGGA
  GAAGTCCAGCGGCCGTACTGCCCATTACAAGTTGACCTCCACGGTGATGCTATGGCTGCAAACCAACAAA
  TCCGGCTCGGGCACCATGAACCTGGGAGGCAGCCTAACCAGACAGATGGAGAAAGACGAAACTGTGAGTG
  ACTGTTCCCCACACATAGCCAACATCGGGCGCCTGGTGGAGGACATGGAAAACAAAATCCGAAGCACGCT
  GAATGAGATCTACTTTGGAAAAACAAAGGACATCGTCAACGGGCTGAGGTCTGTGCAGACGTTTGCAGAC
  AAATCAAAGCAAGAAGCGCTTAAGAACGACCTGGTGGAGGCCTTGAAGAGAAAGCAGCAGTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229106 representing NM_001271405
  Red=Cloning site Green=Tags(s)
  
  MHPSRRSLPFPLNCQLVRVGTADYGGASEQSDQQLDCALDLMRRLPPQQIEKNLSDLIDLVPSLCEDLLS
  SVDQPLKIARDKVVGKDYLLCDYNRDGDSYRSPWSNKYDPPLEDGAMPSARLRKLEVEANNAFDQYRDLY
  FEGGVSSVYLWDLDHGFAGVILIKKAGDGSKKIKGCWDSIHVVEVQEKSSGRTAHYKLTSTVMLWLQTNK
  SGSGTMNLGGSLTRQMEKDETVSDCSPHIANIGRLVEDMENKIRSTLNEIYFGKTKDIVNGLRSVQTFAD
  KSKQEALKNDLVEALKRKQQC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271405
• ORF Size: 903 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271405.1, NP_001258334.1
• RefSeq Size: 1655 bp
• RefSeq ORF: 906 bp
• Locus ID: 12345
• UniProt ID: P47757
• Cytogenetics: 4 70.59 cM
• MW: 34.2 kDa
• Gene Summary: This gene encodes the beta subunit of a highly conserved filamentous actin capping protein that binds the barbed end of filamentous actin to stabilize it and terminate elongation. Interaction of this protein with the barbed end of the actin filament occurs through binding of the amphipathic helix at the C-terminus to the hydrophobic cleft on the actin molecule. This gene is required for a variety of dynamic actin-mediated processes including organization of lamellipodia and filopodia, growth cone morphology and neurite outgrowth in hippocampal neurons, and asymmetric spindle migration and polar body extrusion during oocyte maturation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]