Tbc1d7 (NM_001252639) Mouse Tagged ORF Clone

CAT#: MR229059

Tbc1d7 (myc-DDK-tagged) - Mouse TBC1 domain family, member 7 (Tbc1d7), transcript variant 1

Product Images

Circular map for MR229059

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tbc1d7
• Synonyms: 2610009C09Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229059 representing NM_001252639
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGACGACTCTCAGAGGAACTTTCGATCAGTCTACTATGAGAAAGTCGGGTTTCGTGGTGTCGAAG
  AAAAGAAATCACTGGAAATCCTCCTGAAAGATGACCGTTTGGACATCGAGAAGCTTTGCACATTTAGCCA
  GAGGTTCCCTCTCCCATCCATGTATCGCGCGTTGGTATGGAAGGCGCTTCTAGGCATCTTACCTCCGCAC
  CATGACACTCATTCCCAGGTGATGGCCTACCGCAAAGACCAGTACCATGACATCCTCCATGCCCTGACAG
  TCGTCCGCTTCATCAGTGATGCCACGCCACAGGCTGAAGTGTATCTTCGCATGTATCAGCTTGAATCGGG
  GAAGCTACCTCGAAGTCCCTCTTTTCCTCTGGAGCCGGAGGATGAAGTCTTTCTTGCCATCGCCAAGGCC
  ATGGAAGAGATGGTGGAAGACAGTGTGGACTGTTACTGGATCAGCCGATGCTTCGTGAAGCAGTTAAATA
  ACAAGTACAGGGACGCTTTACCTCAGCTGCCCAAGGCTTTCGAGCAGTACTTGAATCTGGAAGACAGTAG
  GCTGCTGAGTCACCTGAAGACGTGTTCTGCAGTGTCCAAACTGCCTTACGACCTCTGGTTCCAAAGGTGC
  TTCGCGGGATGCCTCCCCGAGTCCAGTTTACAGAGGGTCTGGGATAAAGTCGTAAGTGGATCCTGTAAGA
  TCCTAGTTTTTGTAGCGGTAGAAATACTATTAACCTTTAAAATAAAGGTCATGGCATTGAACAGTGCAGA
  GAAGATAACGAAGTTCCTGGAAAATATTCCTCAGGACAGCTCAGATGCCATCGTGAGCAAGGCCATCGAC
  TTGTGGCACAAACACTGTGGGACCCCAGTGCATTCGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229059 representing NM_001252639
  Red=Cloning site Green=Tags(s)
  
  MTDDSQRNFRSVYYEKVGFRGVEEKKSLEILLKDDRLDIEKLCTFSQRFPLPSMYRALVWKALLGILPPH
  HDTHSQVMAYRKDQYHDILHALTVVRFISDATPQAEVYLRMYQLESGKLPRSPSFPLEPEDEVFLAIAKA
  MEEMVEDSVDCYWISRCFVKQLNNKYRDALPQLPKAFEQYLNLEDSRLLSHLKTCSAVSKLPYDLWFQRC
  FAGCLPESSLQRVWDKVVSGSCKILVFVAVEILLTFKIKVMALNSAEKITKFLENIPQDSSDAIVSKAID
  LWHKHCGTPVHSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252639
• ORF Size: 879 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252639.1, NP_001239568.1
• RefSeq Size: 1268 bp
• RefSeq ORF: 882 bp
• Locus ID: 67046
• UniProt ID: Q9D0K0
• Cytogenetics: 13 A4
• MW: 33.8 kDa
• Gene Summary: Component of the TSC-TBC complex, that contains TBC1D7 in addition to the TSC1-TSC2 complex and consists of the functional complex possessing GTPase-activating protein (GAP) activity toward RHEB in response to alterations in specific cellular growth conditions. The small GTPase RHEB is a direct activator of the protein kinase activity of mTORC1 and the TSC-TBC complex acts as a negative regulator of mTORC1 signaling cascade by acting as a GAP for RHEB. Participates in the proper sensing of growth factors and glucose, but not amino acids, by mTORC1. It is unclear whether TBC1D7 acts as a GTPase-activating protein and additional studies are required to answer this question.[UniProtKB/Swiss-Prot Function]