Srsf5 (NM_001079694) Mouse Tagged ORF Clone

CAT#: MR228912

Srsf5 (myc-DDK-tagged) - Mouse serine/arginine-rich splicing factor 5 (Srsf5), transcript variant 2

Product Images

Circular map for MR228912

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Srsf5
• Synonyms: Sfrs5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228912 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCTGTCGAGTGTTCATTGGGAGACTAAATCCAGCAGCGAGGGAGAAAGATGTGGAAAGATTCT
  TCAAGGGTTACGGACGGATCCGAGATATTGACTTGAAAAGAGGTTTTGGTTTTGTGGAATTTGAGGACCC
  AAGGGATGCAGATGATGCTGTTTATGAACTTGATGGGAAGGAACTTTGCAGTGAAAGGGTGACGATTGAA
  CATGCCCGGGCTCGGTCTCGAGGTGGAAGAGGTAGAGGACGATACTCCGACCGTTTTAGCAGTCGCAGAC
  CTCGAAATGATAGACGAAATGCTCCACCTGTAAGAACAGAAAATCGACTTATAGTTGAGAATTTATCCTC
  AAGAGTCAGCTGGCAGGATCTCAAAGATTTCATGAGACAAGCTGGGGAAGTAACCTTTGCGGATGCACAT
  CGACCTAAACTAAATGAAGGGGTAGTTGAGTTTGCCTCTTATGGTGACTTAAAGAATGCTATTGAGAAAC
  TTTCTGGAAAGGAAATTAACGGGAGAAAAATCAAATTAATTGAAGGCAGCAAAAGGCACAGGTCAAGAAG
  CAGGTCACGATCTCGGACCAGAAGTTCCTCTAGGTCCCGTAGCCGATCCCGTTCACGAAGGAGCAAGTCT
  TACAGCCGATCAAGGAGCAGGAGCCGGAGCCGGAGCAAGTCCCGTTCTGGTAGTAGGTCTCCTGTGCCTG
  AGAAGAGCCAGAAACGTGGTTCTTCGAGTAGATCTAAATCTCCAGCATCTGTGGATCGCCAGAGGTCCCG
  ATCCCGGTCCAGGTCCAGATCAGTTGACAGTGGCAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228912 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGCRVFIGRLNPAAREKDVERFFKGYGRIRDIDLKRGFGFVEFEDPRDADDAVYELDGKELCSERVTIE
  HARARSRGGRGRGRYSDRFSSRRPRNDRRNAPPVRTENRLIVENLSSRVSWQDLKDFMRQAGEVTFADAH
  RPKLNEGVVEFASYGDLKNAIEKLSGKEINGRKIKLIEGSKRHRSRSRSRSRTRSSSRSRSRSRSRRSKS
  YSRSRSRSRSRSKSRSGSRSPVPEKSQKRGSSSRSKSPASVDRQRSRSRSRSRSVDSGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079694
• ORF Size: 810 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079694.2
• RefSeq Size: 1536 bp
• RefSeq ORF: 810 bp
• Locus ID: 20384
• UniProt ID: O35326
• Cytogenetics: 12 D1
• MW: 30.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. [provided by RefSeq, Nov 2016]