Dram2 (NM_001286987) Mouse Tagged ORF Clone

CAT#: MR228901

Dram2 (myc-DDK-tagged) - Mouse DNA-damage regulated autophagy modulator 2 (Dram2), transcript variant 4

Product Images

Circular map for MR228901

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dram2
• Synonyms: 2010305N14Rik; 2610318G18Rik; AI647667; Tmem77
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228901 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGTGGTTTCAGCAAGGCCTCAGTTTTCTTCCGTCGGCCCTTGTAATTTGGACATTTGCTACCTTCA
  TATTCTCATATATCACTGCAATAACACTCCACCATGTTGACCCTGCATTGCCTTATATCAGCGACACAGG
  GACAATACCTCCTGAAAGATGCCTCTTTGGAGTAATGCTAAATATCGCTGCAGTTTTAGGCATTGCTACC
  ATGTATGTTCGTTACAAGCAAGTTCATGCACTGAACCCTGAAGAGAACCTTATCATCAAATTAAACAAGG
  CTGGCCTTGTACTTGGGATACTGAGTTGTTTAGGACTTTCCCTTGTGGCAAATTTCCAGAAATCAACACT
  ATTTATTGTACATGTGTGTGGAGCTGTCCTTGCCTTTAGTATGGGCTCATTTTACATGTTTGTTCAGACA
  ATCCTTTCCTACCAAATGCAGCCCAAAATTCACAGCAAACAAGTCTTCTGGGTCCGACTACTGTTGGTTA
  TCTGGTGTGGAGTAAGTGCACTTAGCATGATGACTTGCTCATCAATTTTGTACAGTAGTGATTTTGGTCC
  TGATGTAGTACAGAAACTACACTGGAACCCGGAGGACAAAGGCTATGTGCTTCACCTGGTTACTACGGCA
  GCAGAATGGTCCATGTCATTTTCCTTTTTTGGATTTTTCCTTACTTATATTCGTGATTTTCAGAAAATTA
  CTTTGCGGGTGGAAGCCAATTTACATGGATTAACCCTCTATGACACTGTTCCTTGCCCTGTTAACAATGA
  AAGAACACCACTGCTTTCCAGAGATTTTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228901 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWWFQQGLSFLPSALVIWTFATFIFSYITAITLHHVDPALPYISDTGTIPPERCLFGVMLNIAAVLGIAT
  MYVRYKQVHALNPEENLIIKLNKAGLVLGILSCLGLSLVANFQKSTLFIVHVCGAVLAFSMGSFYMFVQT
  ILSYQMQPKIHSKQVFWVRLLLVIWCGVSALSMMTCSSILYSSDFGPDVVQKLHWNPEDKGYVLHLVTTA
  AEWSMSFSFFGFFLTYIRDFQKITLRVEANLHGLTLYDTVPCPVNNERTPLLSRDFQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286987
• ORF Size: 801 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286987.1, NP_001273916.1
• RefSeq Size: 2597 bp
• RefSeq ORF: 804 bp
• Locus ID: 67171
• UniProt ID: Q9CR48
• Cytogenetics: 3 F2.3
• MW: 30.2 kDa
• Gene Summary: Plays a role in the initiation of autophagy. In the retina, might be involved in the process of photoreceptor cells renewal and recycling to preserve visual function. Induces apoptotic cell death when coexpressed with DRAM1.[UniProtKB/Swiss-Prot Function]