Gdnf (NM_001301333) Mouse Tagged ORF Clone

CAT#: MR228653

Gdnf (myc-DDK-tagged) - Mouse glial cell line derived neurotrophic factor (Gdnf), transcript variant 3

Product Images

Circular map for MR228653

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gdnf
• Synonyms: AI385739; ATF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228653 representing NM_001301333
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAATGAATTCGTCTCCGCAGCAGTGGGAGGTGCCGCCGCCGGACGGGACTCTAAGATGAAGTTAT
  GGGATGTCGTGGCTGTCTGCCTGGTGTTGCTCCACACCGCGTCTGCCTTCCCGCTGCCCGCCGGTAAGAG
  GCTTCTCGAAGCGCCCGCTGAAGACCACTCCCTCGGCCACCGCCGCGTGCCCTTCGCGCTGACCAGTGAC
  TCCAATATGCCTGAAGATTATCCTGACCAGTTTGATGACGTCATGGATTTTATTCAAGCCACCATTAAAA
  GACTGAAAAGGTCACCAGATAAACAAGCGGCAGCGCTTCCTCGAAGAGAGAGGAATCGGCAGGCTGCAGC
  TGCCAGCCCAGAGAATTCCAGAGGGAAAGGTCGCAGAGGCCAGAGGGGCAAAAATCGGGGGTGCGTTTTA
  ACTGCCATACACTTAAATGTCACTGACTTGGGTTTGGGCTATGAAACCAAGGAGGAACTGATCTTTCGAT
  ATTGCAGCGGTTCCTGTGAATCGGCCGAGACAATGTATGACAAAATACTAAAAAACCTGTCTCGGAGTAG
  AAGGCTAACAAGTGACAAAGTAGGCCAGGCATGTTGCAGGCCGGTCGCCTTCGACGACGACCTGTCGTTT
  TTAGATGACAACCTGGTTTACCATATTCTAAGAAAGCATTCCGCTAAACGGTGTGGATGTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228653 representing NM_001301333
  Red=Cloning site Green=Tags(s)
  
  MENEFVSAAVGGAAAGRDSKMKLWDVVAVCLVLLHTASAFPLPAGKRLLEAPAEDHSLGHRRVPFALTSD
  SNMPEDYPDQFDDVMDFIQATIKRLKRSPDKQAAALPRRERNRQAAAASPENSRGKGRRGQRGKNRGCVL
  TAIHLNVTDLGLGYETKEELIFRYCSGSCESAETMYDKILKNLSRSRRLTSDKVGQACCRPVAFDDDLSF
  LDDNLVYHILRKHSAKRCGCI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301333
• ORF Size: 693 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301333.1, NP_001288262.1
• RefSeq Size: 3761 bp
• RefSeq ORF: 696 bp
• Locus ID: 14573
• Cytogenetics: 15 A1
• MW: 26.1 kDa
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Homozygous knockout mice for this gene exhibit defects in kidney development and neonatal death. This gene encodes multiple protein isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016]