Arl4a (NM_007487) Mouse Tagged ORF Clone

CAT#: MR228463

Arl4a (myc-DDK-tagged) - Mouse ADP-ribosylation factor-like 4A (Arl4a), transcript variant 1

Product Images

Circular map for MR228463

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arl4a
• Synonyms: AI467555; Arl4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228463 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAATGGACTGTCAGACCAGACTTCCATCCTGTCCAGCCTGCCGTCCTTTCAGTCCTTTCACATTG
  TTATTCTGGGTTTGGACTGTGCTGGAAAGACAACAGTTTTATACAGGCTGCAGTTCAACGAATTTGTAAA
  TACCGTACCTACCAAAGGATTTAACACTGAGAAAATTAAGGTAACCTTGGGCAATTCCAAAACAGTCACT
  TTTCACTTCTGGGATGTAGGTGGTCAAGAGAAGTTAAGACCACTGTGGAAGTCATACACCCGATGCACAG
  ATGGTATTGTGTTTGTGGTGGACTCTGTCGATGTTGAGAGAATGGAAGAAGCCAAAACTGAACTTCATAA
  AATAACTAGGATATCCGAAAATCAGGGAGTCCCTGTGCTTATAGTTGCTAATAAACAAGACCTGAGGAAC
  TCGCTCTCTCTCTCAGAGATTGAGAAGTTGTTAGCAATGGGGGAACTGAGCTCATCGACTCCTTGGCATT
  TGCAGCCCACCTGTGCAATCATAGGAGATGGGCTAAAGGAAGGACTCGAGAAACTACATGATATGATAAT
  TAAAAGGAGAAAAATGTTGCGGCAACAGAAAAAGAAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228463 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGNGLSDQTSILSSLPSFQSFHIVILGLDCAGKTTVLYRLQFNEFVNTVPTKGFNTEKIKVTLGNSKTVT
  FHFWDVGGQEKLRPLWKSYTRCTDGIVFVVDSVDVERMEEAKTELHKITRISENQGVPVLIVANKQDLRN
  SLSLSEIEKLLAMGELSSSTPWHLQPTCAIIGDGLKEGLEKLHDMIIKRRKMLRQQKKKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007487
• ORF Size: 603 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007487.3, NP_031513.1
• RefSeq Size: 3657 bp
• RefSeq ORF: 603 bp
• Locus ID: 11861
• UniProt ID: P61213
• Cytogenetics: 12 18.06 cM
• MW: 22.6 kDa
• Gene Summary: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form (By similarity).[UniProtKB/Swiss-Prot Function]