Fgf13 (NM_001290415) Mouse Tagged ORF Clone

CAT#: MR228408

Fgf13 (myc-DDK-tagged) - Mouse fibroblast growth factor 13 (Fgf13), transcript variant 3

Product Images

Circular map for MR228408

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fgf13
• Synonyms: Fhf2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228408 representing NM_001290415
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTGTTAAGGAAGTCATATTCAGAGCCTCAGCTTAAGGGTATAGTTACCAAACTATACAGCCGAC
  AAGGCTACCACTTGCAACTGCAGGCAGATGGAACCATTGATGGCACCAAAGACGAGGACAGCACTTACAC
  TCTGTTTAACCTCATCCCTGTGGGTCTTCGGGTGGTGGCTATTCAAGGAGTTCAAACCAAGCTGTATTTG
  GCAATGAACAGCGAGGGATACTTGTACACCTCGGAACATTTCACACCTGAGTGCAAATTCAAAGAATCAG
  TGTTTGAAAATTATTACGTGACATACTCATCAATGATCTACCGTCAGCAGCAATCCGGCCGAGGGTGGTA
  TCTAGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCTGCAGCACAT
  TTTCTGCCCAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCTCTGCACGATCTCACGGAGTTCTCCC
  GATCTGGAAGTGGGACCCCGACCAAGAGCAGAAGCGTCTCTGGTGTACTGAATGGAGGCAAATCCATGAG
  CCACAACGAATCAACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228408 representing NM_001290415
  Red=Cloning site Green=Tags(s)
  
  MALLRKSYSEPQLKGIVTKLYSRQGYHLQLQADGTIDGTKDEDSTYTLFNLIPVGLRVVAIQGVQTKLYL
  AMNSEGYLYTSEHFTPECKFKESVFENYYVTYSSMIYRQQQSGRGWYLGLNKEGEIMKGNHVKKNKPAAH
  FLPKPLKVAMYKEPSLHDLTEFSRSGSGTPTKSRSVSGVLNGGKSMSHNEST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290415
• ORF Size: 576 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290415.1, NP_001277344.1
• RefSeq Size: 2025 bp
• RefSeq ORF: 579 bp
• Locus ID: 14168
• UniProt ID: P70377
• Cytogenetics: X 33.31 cM
• MW: 22.1 kDa
• Gene Summary: Microtubule-binding protein which directly binds tubulin and is involved in both polymerization and stabilization of microtubules. Through its action on microtubules, may participate to the refinement of axons by negatively regulating axonal and leading processes branching. Plays a crucial role in neuron polarization and migration in the cerebral cortex and the hippocampus.[UniProtKB/Swiss-Prot Function]