Ahr (NM_013464) Mouse Tagged ORF Clone

CAT#: MR227590

Ahr (Myc-DDK-tagged) - Mouse aryl-hydrocarbon receptor (Ahr)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ahr
• Synonyms: A; Ah; Ahh; Ahre; bHLHe7; bHLHe76; In
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227590 representing NM_013464
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGCGGCGCCAACATCACCTATGCCAGCCGCAAGCGGCGCAAGCCGGTGCAGAAAACAGTAAAGC
  CCATCCCCGCTGAAGGAATTAAGTCAAATCCTTCTAAGCGACACAGAGACCGGCTGAACACAGAGTTAGA
  CCGCCTGGCCAGCCTGCTGCCCTTCCCGCAAGATGTTATTAATAAGCTGGACAAACTCTCTGTTCTTAGG
  CTCAGCGTCAGCTACCTGAGGGCCAAGAGCTTCTTTGATGTTGCATTAAAGTCCACCCCTGCTGACAGAA
  ATGGAGGCCAGGACCAGTGTAGAGCACAAATCAGAGACTGGCAGGATTTGCAAGAAGGAGAGTTCTTGTT
  ACAGGCGCTGAATGGCTTTGTGCTGGTTGTCACAGCAGATGCCTTGGTCTTCTATGCTTCCTCCACTATC
  CAAGATTACCTGGGCTTTCAGCAGTCTGATGTCATCCATCAGAGCGTATATGAGCTCATCCATACAGAAG
  ACCGGGCGGAATTCCAGCGCCAGCTTCACTGGGCTCTAAACCCAGACTCTGCACAAGGAGTGGACGAAGC
  CCATGGCCCTCCACAGGCAGCAGTCTATTATACCCCAGACCAGCTTCCTCCAGAGAACGCTTCTTTCATG
  GAGAGGTGCTTCAGGTGCCGGCTGAGGTGCCTGCTGGATAATTCATCTGGTTTTCTGGCAATGAATTTCC
  AAGGGAGGTTAAAGTATCTTCATGGACAGAACAAGAAAGGGAAGGACGGAGCGCTGCTTCCTCCACAACT
  GGCTTTGTTTGCAATAGCTACTCCACTTCAGCCACCCTCCATCCTGGAAATTCGAACCAAAAACTTCATC
  TTCAGGACCAAACACAAGCTAGACTTCACACCTATTGGTTGTGATGCCAAAGGGCAGCTTATTCTGGGCT
  ATACAGAAGTAGAGCTGTGCACAAGAGGATCGGGGTACCAGTTCATCCATGCTGCAGACATACTTCACTG
  TGCAGAATCCCACATCCGCATGATTAAGACTGGAGAAAGTGGCATGACAGTTTTCCGGCTTCTTGCAAAA
  CACAGTCGCTGGAGGTGGGTCCAGTCCAATGCACGCTTGATTTACAGAAATGGAAGACCAGATTACATCA
  TCGCCACTCAGAGACCACTGACGGATGAAGAAGGACGAGAGCATTTACAGAAGCGAAGTACGTCGCTGCC
  CTTCATGTTTGCTACCGGAGAGGCTGTGTTGTACGAGATCTCCAGCCCTTTCTCTCCCATAATGGATCCC
  CTACCAATACGCACCAAAAGCAACACTAGCAGGAAAGACTGGGCTCCCCAGTCAACCCCAAGTAAGGATT
  CTTTCCACCCCAGTTCTCTTATGAGTGCCCTCATCCAGCAGGATGAGTCCATCTATCTGTGTCCTCCTTC
  AAGCCCTGCGCTGTTAGACAGCCATTTTCTCATGGGCTCCGTGAGCAAGTGCGGGAGTTGGCAAGACAGC
  TTTGCGGCCGCAGGAAGTGAGGCTGCGCTGAAACATGAGCAAATTGGCCATGCTCAGGACGTGAACCTTG
  CACTCTCTGGCGGCCCCTCAGAGCTCTTTCCGGATAATAAAAATAATGACTTGTACAGCATCATGAGGAA
  CCTTGGGATTGATTTTGAAGATATCAGAAGCATGCAGAACGAGGAGTTCTTCAGAACTGACTCCACCGCT
  GCTGGTGAGGTTGACTTCAAAGACATCGACATAACGGACGAAATCCTGACCTACGTGCAGGATTCCCTGA
  ACAATTCAACTTTGCTGAACTCGGCTTGCCAGCAGCAGCCTGTGACTCAGCACCTAAGCTGTATGCTGCA
  GGAGCGCCTGCAACTAGAGCAACAGCAACAGCTTCAGCAGCCCCCGCCGCAGGCTCTGGAGCCCCAGCAG
  CAGCTGTGTCAGATGGTGTGCCCCCAGCAAGATCTGGGTCCGAAGCACACGCAAATCAACGGCACGTTTG
  CAAGTTGGAACCCCACCCCTCCCGTGTCTTTCAACTGTCCCCAGCAGGAACTAAAGCACTATCAGCTCTT
  TTCCAGCTTACAGGGGACTGCTCAGGAATTTCCCTACAAACCAGAGGTGGACAGTGTGCCTTACACACAG
  AACTTTGCTCCCTGTAATCAGCCTCTGCTTCCAGAACATTCCAAGAGTGTGCAGTTGGACTTCCCTGGAA
  GGGATTTTGAACCGTCCCTGCATCCCACTACTTCTAATTTAGATTTTGTCAGTTGTTTACAAGTTCCTGA
  AAACCAAAGTCATGGGATAAACTCACAGTCCGCCATGGTCAGTCCTCAGGCATACTATGCTGGGGCCATG
  TCCATGTATCAGTGCCAGCCAGGGCCACAGCGCACCCCTGTGGACCAGACGCAGTACAGCTCTGAAATTC
  CAGGTTCTCAGGCATTCCTAAGCAAGGTGCAGAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227590 representing NM_013464
  Red=Cloning site Green=Tags(s)
  
  MSSGANITYASRKRRKPVQKTVKPIPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLR
  LSVSYLRAKSFFDVALKSTPADRNGGQDQCRAQIRDWQDLQEGEFLLQALNGFVLVVTADALVFYASSTI
  QDYLGFQQSDVIHQSVYELIHTEDRAEFQRQLHWALNPDSAQGVDEAHGPPQAAVYYTPDQLPPENASFM
  ERCFRCRLRCLLDNSSGFLAMNFQGRLKYLHGQNKKGKDGALLPPQLALFAIATPLQPPSILEIRTKNFI
  FRTKHKLDFTPIGCDAKGQLILGYTEVELCTRGSGYQFIHAADILHCAESHIRMIKTGESGMTVFRLLAK
  HSRWRWVQSNARLIYRNGRPDYIIATQRPLTDEEGREHLQKRSTSLPFMFATGEAVLYEISSPFSPIMDP
  LPIRTKSNTSRKDWAPQSTPSKDSFHPSSLMSALIQQDESIYLCPPSSPALLDSHFLMGSVSKCGSWQDS
  FAAAGSEAALKHEQIGHAQDVNLALSGGPSELFPDNKNNDLYSIMRNLGIDFEDIRSMQNEEFFRTDSTA
  AGEVDFKDIDITDEILTYVQDSLNNSTLLNSACQQQPVTQHLSCMLQERLQLEQQQQLQQPPPQALEPQQ
  QLCQMVCPQQDLGPKHTQINGTFASWNPTPPVSFNCPQQELKHYQLFSSLQGTAQEFPYKPEVDSVPYTQ
  NFAPCNQPLLPEHSKSVQLDFPGRDFEPSLHPTTSNLDFVSCLQVPENQSHGINSQSAMVSPQAYYAGAM
  SMYQCQPGPQRTPVDQTQYSSEIPGSQAFLSKVQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013464
• ORF Size: 2415 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013464.4, NP_038492.1
• RefSeq Size: 5494 bp
• RefSeq ORF: 2418 bp
• Locus ID: 11622
• UniProt ID: P30561
• Cytogenetics: 12 15.78 cM
• MW: 90.8 kDa
• Gene Summary: The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]