Ahr (NM_013464) Mouse Tagged ORF Clone

USD 850.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Ahr (NM_013464) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Ahr
Synonyms Ah; Ahh; Ahre; bHLHe76; In
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR227590 representing NM_013464
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCAGCGGCGCCAACATCACCTATGCCAGCCGCAAGCGGCGCAAGCCGGTGCAGAAAACAGTAAAGC
CCATCCCCGCTGAAGGAATTAAGTCAAATCCTTCTAAGCGACACAGAGACCGGCTGAACACAGAGTTAGA
CCGCCTGGCCAGCCTGCTGCCCTTCCCGCAAGATGTTATTAATAAGCTGGACAAACTCTCTGTTCTTAGG
CTCAGCGTCAGCTACCTGAGGGCCAAGAGCTTCTTTGATGTTGCATTAAAGTCCACCCCTGCTGACAGAA
ATGGAGGCCAGGACCAGTGTAGAGCACAAATCAGAGACTGGCAGGATTTGCAAGAAGGAGAGTTCTTGTT
ACAGGCGCTGAATGGCTTTGTGCTGGTTGTCACAGCAGATGCCTTGGTCTTCTATGCTTCCTCCACTATC
CAAGATTACCTGGGCTTTCAGCAGTCTGATGTCATCCATCAGAGCGTATATGAGCTCATCCATACAGAAG
ACCGGGCGGAATTCCAGCGCCAGCTTCACTGGGCTCTAAACCCAGACTCTGCACAAGGAGTGGACGAAGC
CCATGGCCCTCCACAGGCAGCAGTCTATTATACCCCAGACCAGCTTCCTCCAGAGAACGCTTCTTTCATG
GAGAGGTGCTTCAGGTGCCGGCTGAGGTGCCTGCTGGATAATTCATCTGGTTTTCTGGCAATGAATTTCC
AAGGGAGGTTAAAGTATCTTCATGGACAGAACAAGAAAGGGAAGGACGGAGCGCTGCTTCCTCCACAACT
GGCTTTGTTTGCAATAGCTACTCCACTTCAGCCACCCTCCATCCTGGAAATTCGAACCAAAAACTTCATC
TTCAGGACCAAACACAAGCTAGACTTCACACCTATTGGTTGTGATGCCAAAGGGCAGCTTATTCTGGGCT
ATACAGAAGTAGAGCTGTGCACAAGAGGATCGGGGTACCAGTTCATCCATGCTGCAGACATACTTCACTG
TGCAGAATCCCACATCCGCATGATTAAGACTGGAGAAAGTGGCATGACAGTTTTCCGGCTTCTTGCAAAA
CACAGTCGCTGGAGGTGGGTCCAGTCCAATGCACGCTTGATTTACAGAAATGGAAGACCAGATTACATCA
TCGCCACTCAGAGACCACTGACGGATGAAGAAGGACGAGAGCATTTACAGAAGCGAAGTACGTCGCTGCC
CTTCATGTTTGCTACCGGAGAGGCTGTGTTGTACGAGATCTCCAGCCCTTTCTCTCCCATAATGGATCCC
CTACCAATACGCACCAAAAGCAACACTAGCAGGAAAGACTGGGCTCCCCAGTCAACCCCAAGTAAGGATT
CTTTCCACCCCAGTTCTCTTATGAGTGCCCTCATCCAGCAGGATGAGTCCATCTATCTGTGTCCTCCTTC
AAGCCCTGCGCTGTTAGACAGCCATTTTCTCATGGGCTCCGTGAGCAAGTGCGGGAGTTGGCAAGACAGC
TTTGCGGCCGCAGGAAGTGAGGCTGCGCTGAAACATGAGCAAATTGGCCATGCTCAGGACGTGAACCTTG
CACTCTCTGGCGGCCCCTCAGAGCTCTTTCCGGATAATAAAAATAATGACTTGTACAGCATCATGAGGAA
CCTTGGGATTGATTTTGAAGATATCAGAAGCATGCAGAACGAGGAGTTCTTCAGAACTGACTCCACCGCT
GCTGGTGAGGTTGACTTCAAAGACATCGACATAACGGACGAAATCCTGACCTACGTGCAGGATTCCCTGA
ACAATTCAACTTTGCTGAACTCGGCTTGCCAGCAGCAGCCTGTGACTCAGCACCTAAGCTGTATGCTGCA
GGAGCGCCTGCAACTAGAGCAACAGCAACAGCTTCAGCAGCCCCCGCCGCAGGCTCTGGAGCCCCAGCAG
CAGCTGTGTCAGATGGTGTGCCCCCAGCAAGATCTGGGTCCGAAGCACACGCAAATCAACGGCACGTTTG
CAAGTTGGAACCCCACCCCTCCCGTGTCTTTCAACTGTCCCCAGCAGGAACTAAAGCACTATCAGCTCTT
TTCCAGCTTACAGGGGACTGCTCAGGAATTTCCCTACAAACCAGAGGTGGACAGTGTGCCTTACACACAG
AACTTTGCTCCCTGTAATCAGCCTCTGCTTCCAGAACATTCCAAGAGTGTGCAGTTGGACTTCCCTGGAA
GGGATTTTGAACCGTCCCTGCATCCCACTACTTCTAATTTAGATTTTGTCAGTTGTTTACAAGTTCCTGA
AAACCAAAGTCATGGGATAAACTCACAGTCCGCCATGGTCAGTCCTCAGGCATACTATGCTGGGGCCATG
TCCATGTATCAGTGCCAGCCAGGGCCACAGCGCACCCCTGTGGACCAGACGCAGTACAGCTCTGAAATTC
CAGGTTCTCAGGCATTCCTAAGCAAGGTGCAGAGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR227590 representing NM_013464
Red=Cloning site Green=Tags(s)

MSSGANITYASRKRRKPVQKTVKPIPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLR
LSVSYLRAKSFFDVALKSTPADRNGGQDQCRAQIRDWQDLQEGEFLLQALNGFVLVVTADALVFYASSTI
QDYLGFQQSDVIHQSVYELIHTEDRAEFQRQLHWALNPDSAQGVDEAHGPPQAAVYYTPDQLPPENASFM
ERCFRCRLRCLLDNSSGFLAMNFQGRLKYLHGQNKKGKDGALLPPQLALFAIATPLQPPSILEIRTKNFI
FRTKHKLDFTPIGCDAKGQLILGYTEVELCTRGSGYQFIHAADILHCAESHIRMIKTGESGMTVFRLLAK
HSRWRWVQSNARLIYRNGRPDYIIATQRPLTDEEGREHLQKRSTSLPFMFATGEAVLYEISSPFSPIMDP
LPIRTKSNTSRKDWAPQSTPSKDSFHPSSLMSALIQQDESIYLCPPSSPALLDSHFLMGSVSKCGSWQDS
FAAAGSEAALKHEQIGHAQDVNLALSGGPSELFPDNKNNDLYSIMRNLGIDFEDIRSMQNEEFFRTDSTA
AGEVDFKDIDITDEILTYVQDSLNNSTLLNSACQQQPVTQHLSCMLQERLQLEQQQQLQQPPPQALEPQQ
QLCQMVCPQQDLGPKHTQINGTFASWNPTPPVSFNCPQQELKHYQLFSSLQGTAQEFPYKPEVDSVPYTQ
NFAPCNQPLLPEHSKSVQLDFPGRDFEPSLHPTTSNLDFVSCLQVPENQSHGINSQSAMVSPQAYYAGAM
SMYQCQPGPQRTPVDQTQYSSEIPGSQAFLSKVQS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_013464
ORF Size 2415 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_013464.1, NM_013464.2, NM_013464.3, NM_013464.4, NP_038492.1
RefSeq Size 5494 bp
RefSeq ORF 2418 bp
Locus ID 11622
Cytogenetics 12 15.78 cM
MW 90.8 kDa
Gene Summary The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Citations (1)

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