Prnp (NM_011170) Mouse Tagged ORF Clone

CAT#: MR227504

Prnp (Myc-DDK-tagged) - Mouse prion protein (Prnp)

Product Images

Circular map for MR227504

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prnp
• Synonyms: AA960666; AI325101; CD230; Prn-i; Prn-p; PrP; prP27-30; prP33-35C; PrP; PrPC; PrPSc; Sinc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227504 representing NM_011170
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAACCTTGGCTACTGGCTGCTGGCCCTCTTTGTGACTATGTGGACTGATGTCGGCCTCTGCAAAA
  AGCGGCCAAAGCCTGGAGGGTGGAACACCGGTGGAAGCCGGTATCCCGGGCAGGGAAGCCCTGGAGGCAA
  CCGTTACCCACCTCAGGGTGGCACCTGGGGGCAGCCCCACGGTGGTGGCTGGGGACAACCCCATGGGGGC
  AGCTGGGGACAACCTCATGGTGGTAGTTGGGGTCAGCCCCATGGCGGTGGATGGGGCCAAGGAGGGGGTA
  CCCATAATCAGTGGAACAAGCCCAGCAAACCAAAAACCAACCTCAAGCATGTGGCAGGGGCTGCGGCAGC
  TGGGGCAGTAGTGGGGGGCCTTGGTGGCTACATGCTGGGGAGCGCCATGAGCAGGCCCATGATCCATTTT
  GGCAACGACTGGGAGGACCGCTACTACCGTGAAAACATGTACCGCTACCCTAACCAAGTGTACTACAGGC
  CAGTGGATCAGTACAGCAACCAGAACAACTTCGTGCACGACTGCGTCAATATCACCATCAAGCAGCACAC
  GGTCACCACCACCACCAAGGGGGAGAACTTCACCGAGACCGATGTGAAGATGATGGAGCGCGTGGTGGAG
  CAGATGTGCGTCACCCAGTACCAGAAGGAGTCCCAGGCCTATTACGACGGGAGAAGATCCAGCAGCACCG
  TGCTTTTCTCCTCCCCTCCTGTCATCCTCCTCATCTCCTTCCTCATCTTCCTGATCGTGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227504 representing NM_011170
  Red=Cloning site Green=Tags(s)
  
  MANLGYWLLALFVTMWTDVGLCKKRPKPGGWNTGGSRYPGQGSPGGNRYPPQGGTWGQPHGGGWGQPHGG
  SWGQPHGGSWGQPHGGGWGQGGGTHNQWNKPSKPKTNLKHVAGAAAAGAVVGGLGGYMLGSAMSRPMIHF
  GNDWEDRYYRENMYRYPNQVYYRPVDQYSNQNNFVHDCVNITIKQHTVTTTTKGENFTETDVKMMERVVE
  QMCVTQYQKESQAYYDGRRSSSTVLFSSPPVILLISFLIFLIVG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011170
• ORF Size: 762 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011170.3
• RefSeq Size: 2206 bp
• RefSeq ORF: 765 bp
• Locus ID: 19122
• UniProt ID: P04925
• Cytogenetics: 2 64.07 cM
• MW: 28.4 kDa
• Gene Summary: Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (PubMed:12732622, PubMed:16492732, PubMed:19242475, PubMed:19568430).[UniProtKB/Swiss-Prot Function]