Nhs (NM_001081052) Mouse Tagged ORF Clone

CAT#: MR227433

Nhs (Myc-DDK-tagged) - Mouse Nance-Horan syndrome (human) (Nhs)

Product Images

Circular map for MR227433

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nhs
• Synonyms: Gm48; Gm389; Nhs1; Xcat
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227433 representing NM_001081052
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTTCGCCAAGCGGATCGTGGAGCCGCAGTGGCTTTGCCGGCAGCGGCGCCCGGCGCCCGGCCCAG
  ATGAGGACACGAGTGGAGGCAGCGTTGAGCCGCCGCCGCCCCTGCAGCCGCCTGGCCGGCGGGAAGAGGC
  CGAGGCACCGGAGCCAGAAGAGCCTCCTCGTGTGCCCCCTGCGCCTCTCCCACTGCCACCTCTGCCGCCG
  TCTTTGCCGGCGCCGGCCGAGCAGGATCAGCAGCCACCCAGCGAAGCGCGGGAGGCCGGGGAGGAGAGCG
  TGGCGGGGGTCTCGGAGGCAGCATCGGCCCCAGGCGAGACGTCCGAGGCTTCAGCCGAGGCTGTGCTGCT
  CATGTTAGACCTGTGCGCGGTCAGCAACGCTGCGCTGGCCAGGGTCCTCCGGCAGCTCTCAGACGTAGCC
  CGGCACGCGTGCAGCCTCTTCCAGGAGCTCGAGAGCGACATCCAGCTTACTCACCGCCGTGTCTGGGCGC
  TGCAGGGCAAGCTCGGTAGCGTGCAGCGCGTCCTCAGCACGTTGGACCCCAAGCAGGAGGCAGTGCCTGT
  CTCCAACTTGGACATCGAGAGCAAGTTGAGTGTATACTACCGTGCACCATGGCACCAACAGCGAAACATC
  TTCCTCCCAGCGACCAGGCCACCCTGTGTAGAAGAGCTGCACCGCCATGCCAGACAGAGTCTGCAAGCCC
  TTCGTAGAGAACACCGGAGCCGGAGTGATCGCAGAGAGCAAAGAGCTGCTGCTCCCCTCCCCATAGCTGC
  TCCACCACTACCAGCCTACCCTCCAGCTCACAGCCAGAGGAGGCGCGAGGCCAAGGACCGCCACTTCCTA
  ACGTCCCACTCTCCAGAGGATGAAGACACAGATGCCATGCTAGGGCAGAGGCCGAAAAACCCAATCCACA
  ATGTTCCCTCTACACTGGATAAGCAGACCAACTGGAACAAAGCACTACCTCTCCCAACTCCTGAGGAGAA
  GATGAAACAAGATGCCCAAGTGATTTCTTCTTGCATCATTCCCATCAATGTCACCGGTGTTGGTTTTGAC
  AGAGAAGCAAGTATACGCTGTTCTCTTGTTCATTCACAATCTGTGCTACAGAGGAGACGAAAACTGAGGA
  GAAGGAAAACAATCTCTGGGATTCCCAGAAGAGTTCAACAAGAAATAGATTCTGATGAATCACCAGTGGC
  CAGGGAAAGGAATGTGATTGTGCACACAAACCCAGACCCCTCCAACACTGTCAATAGGAGGTCCGGAACC
  AGGGACTCTGAATGTCAAACTGAAGATATTCTGATTGCTGCCCCATCCAGAAGGAGAATTAGAGCTCAAA
  GGGGTCAAAGCATCGCAGCTTCCCTTTCTCATTCAGCTGGCAACATCTCTGCCCTGGCAGACAAAGGCGA
  TACCATGTTTACTCCTGTCGTGAGCAGCCGTACAAGATCTCGGAGCCTTCCTCGGGAGGGAAATAGAGGT
  GGAGATGCTGAACCCAAAGTAGGTGCTAAACCCTCAGCATTTGAAGAAGGAGAACGTTTCGTGGGTGACC
  ATGAAAGAACCCCTAATGACTGTAGCGAAGCTCCAAGCAGCCCAAGTACACAGGAACACCAGCCTGCTTT
  GGGCCTAGCATGTTCTCAACATCTTCACAGCCCTCAGCAAAAGTTAAGTGAGAGAGGGAGATCACGTCTG
  TCCCGAATGGCGGCTGACTCTGGAAGCTGTGACATCTCTTCCAACTCGGACACCTTTGGAAGCCCCGTCC
  ATTGCATTTCCACAGCTAGTGTCCTCCTTAGCAGCCACATGGACCAGAAAGAAGACCACCAGTCATCCAG
  TGGTAACTGGAGTGGGAGCAGCTCTACATGCCCGTCACAGACCTCAGAGACAATCCCTCCTGCAGCTTCT
  CCTCCCCTCACGGGCTCTTCTCACTGTGACTCAGAATTGTCACTGAACACTGCTCCTAATGCTAATGAAG
  ATGCCAGTGTCTTTGTGACAGAACAGTACAATGACCACTTGGATAAAGTAAGAGGCCACCGGACAAACTC
  CTTTACTTCCACTGTTGCAGATCTGCTGGATGACCCCAACAACAGCAATACAAGTGACAGTGAGTGGAAT
  TATCTACACCACCATCATGATGCATCTTGCCGCCAAGATTTTAGTCCTGAGCGTCCGAAGGCAGATAGCT
  TGGGATGCCCAAGTTTTACAAGCATGGGCACCTATGACAGCTTTCTGGAAAAGTCTCCTTCAGACAAAGC
  AGATACTAGCTCTCATTTCTCAGTGGACACTGAAGGATACTATACCTCGATGCACTTTGACTGTGGTCTC
  AAAGGTAATAAGACCTATGTCTGTCACTATGCAGGCCTAGGCCCAGAGAATGGTCAGGGTGTCGGGGTTC
  CTCCTGCTCTTCCGGACTGTGCCTGGCAAGAATACTTAGACCACAGGAGGCAGGGGAGACCAAGCATCTC
  TTTCAGGAAACCAAAGGCAAAGCCAACTCCACCTAAACGTAGCTCATCATTGAGAAAATCTGATGGAAAT
  GCAGACATTTCTGAGAAGAAAGAACCAAAGATAAGCAGTGGTCAGCACCTGCCTCACAGTTCCAGGGAAA
  TGAAGCTGCCTCTTGATTTCTCCAACACGCCTTCTCGTGTGGAAAATGCCAATCTGCCTGCAAAGCTGGA
  CTCTTCTTGGATAAACCAGAGTGAACATGCTATTAAGGAACCTCAGTTGGACACTCCAGACATTTCACCA
  TTCAAAGATGAAGGTGCTGAATCAACACACTATGCAGACCTCTGGCTTCTGAATGACTTGAAAACGAATG
  ATCCTTACAGATCCTTATCTAATTCTAGCACTGCTACGGGTACCACAGTGATTGAATGCATCAAATCTCC
  AGAGAGTTCTGAATCCCAAACATCCCAATCAGAATCAAGAGCAACCACTCCATCTCTTCCTTCAGTTGAC
  AATGAATATAAACTGGCTTCACCAGAAAAGCTGGCTGGCTTAGCATCTCCATCAAGTGGCTACTCAAGCC
  AGTCAGAAACGCCAACCTCCTCTTTCCCTACAGCTTTCTTTTCAGGACCATTGTCTCCTGGAGGTAGCAA
  AAGAAAGCCTAAAGTCCCAGAAAGGAAATCCTCACTACAGCAGCCATCTTTAAAAGATGGTGCCCTATCA
  CTGAGTAAAGATTTTGAACTACCAATTATACCTCCTTCTCATCTTGACCTAAGTGCTCTTCATGTTTTGA
  ATAAACCATTCCACCACCGTCACCCACTGCATGTTTTTACTCATAAGCAAAACACAGTCGGAGATATGCC
  GAGATCAAATCCTGCACCGTCTCTTGCAATTACACCAACAGTTCTCAAGTCTGTCAACCTGAGGTCCATC
  AGTAAGTCTGAAGAAGTTAGGCAAAAGGAGGGCAATAATACAGATCTCCCCTACTTAGAAGAGAATGCTG
  CCACAGCGGCTTCTGTGGCCTCCTTATCTCCAAGTAAGGCAAGGCCACACACAGCAAAGAAATCAATATC
  ACGCCAGTATTCTGCTGAAGATACCCTCCTGCCCTTTTTAGATTCCTCTGTAGCTGAGATGGGACCAGAG
  AAGCATTTGGAAAAGAACCCTAATTTTGATGGAAAGAGTCATGGTGATCCAGAAACTGCAACCTCAGCTA
  GCAGCAATCTTCCAGATTCAAATGTGATGAAAGAACAAATACAGATGGAAAGCGAGCTTATCTCAGAAAA
  CATACTAAGTAAGAACTGTGGATTTTCAACAGGATTTCAGAGGGTGTCTGCTTCCCGCCCCAGTGATTTG
  GGTAGTAAAATGATACAGTATGGAGCTAGTCCAGATGGCACTGTACAGCAAGGACAGAAGGCACCCTCTG
  GAGTCAGGGAAGAAGGTGGAAAACCAGAATCTGTGGATGGAATCACACTTCAAGCCAATTCATCAACTAG
  AGTAACAGACATAAGCAGTCAATATAAGCATCAGCGTGTTATAAGCCGCCACCATGACAAAGTGCCTGTG
  ACTATCCGCCATGAGTCAGAGATGTCAACTGTAAATTCATTCCCTGAAAAATGTTCTGAACAGGAAAATA
  TTGCTTCAGGTATTTCACCCCAAAGTGCCTCTGATAACAGCAGAGCAGAGGAGACCCAAGGAAGTATGGA
  CGAGACTTCATTGAAAGAATCCTCACCAAGTGATGACTCCATCACTTCACCACTCAGTGAGGACTCTCAA
  GCTGGAGCTGAGGGTGTGTTTGTGTCTCCAAACAAACCTCGAACCACTGAGGACCTATTTGCAGTCATTC
  ACAGATCCAAGAGGAAAGTACTTGGAAGAAAAGATTCTGGGGATATGTCTGTCAGAAGCAAATCAAGAGT
  TCCCCTTGGTAGCAGTAGCAGCAGTGCCAATTCTGTCACTTCACCCAGCAGCAATGTGACAGCTGGGACT
  AGCCAGAGGTCTCCTGGTCTCATCTACAGAAATGCCAAAAAGTCCAACACATCCAATGAAGAGTTTAAGC
  TGCTGCTCCTCAAGAAAGGCAGTCGTTCAGATTCTAGTTACCGCATGTCTGCTACTGAAATCCTGAAGAG
  CCCGGTCCTGCCCAAGCCTCCTGGGGAGTTTACTGCAGAGTCCCCACAAAGCCCCGATGATACCCATCAG
  GGCACACCTGGGACTGAAGCACTGTCTCCTCTGTCGCCGTGCTCACCACGAGTCAATGCGGAAGGGTTCT
  CCTCGAAGAACTTTGCAACCTCAGCATCAGCACGGGTTGGACGCTCCCGGGTCCCACCTGTGGCCAGCAG
  CAGCCGCTACAGTGTCCGCTGCCGGCTGTACAATACTCCAATGCAGGCCATCTCTGAGGGAGAGACTGAA
  AACTCTGATGGGAGCCCACATGATGACCGATCCTCCCAGAGCTCCACC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227433 representing NM_001081052
  Red=Cloning site Green=Tags(s)
  
  MPFAKRIVEPQWLCRQRRPAPGPDEDTSGGSVEPPPPLQPPGRREEAEAPEPEEPPRVPPAPLPLPPLPP
  SLPAPAEQDQQPPSEAREAGEESVAGVSEAASAPGETSEASAEAVLLMLDLCAVSNAALARVLRQLSDVA
  RHACSLFQELESDIQLTHRRVWALQGKLGSVQRVLSTLDPKQEAVPVSNLDIESKLSVYYRAPWHQQRNI
  FLPATRPPCVEELHRHARQSLQALRREHRSRSDRREQRAAAPLPIAAPPLPAYPPAHSQRRREAKDRHFL
  TSHSPEDEDTDAMLGQRPKNPIHNVPSTLDKQTNWNKALPLPTPEEKMKQDAQVISSCIIPINVTGVGFD
  REASIRCSLVHSQSVLQRRRKLRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPDPSNTVNRRSGT
  RDSECQTEDILIAAPSRRRIRAQRGQSIAASLSHSAGNISALADKGDTMFTPVVSSRTRSRSLPREGNRG
  GDAEPKVGAKPSAFEEGERFVGDHERTPNDCSEAPSSPSTQEHQPALGLACSQHLHSPQQKLSERGRSRL
  SRMAADSGSCDISSNSDTFGSPVHCISTASVLLSSHMDQKEDHQSSSGNWSGSSSTCPSQTSETIPPAAS
  PPLTGSSHCDSELSLNTAPNANEDASVFVTEQYNDHLDKVRGHRTNSFTSTVADLLDDPNNSNTSDSEWN
  YLHHHHDASCRQDFSPERPKADSLGCPSFTSMGTYDSFLEKSPSDKADTSSHFSVDTEGYYTSMHFDCGL
  KGNKTYVCHYAGLGPENGQGVGVPPALPDCAWQEYLDHRRQGRPSISFRKPKAKPTPPKRSSSLRKSDGN
  ADISEKKEPKISSGQHLPHSSREMKLPLDFSNTPSRVENANLPAKLDSSWINQSEHAIKEPQLDTPDISP
  FKDEGAESTHYADLWLLNDLKTNDPYRSLSNSSTATGTTVIECIKSPESSESQTSQSESRATTPSLPSVD
  NEYKLASPEKLAGLASPSSGYSSQSETPTSSFPTAFFSGPLSPGGSKRKPKVPERKSSLQQPSLKDGALS
  LSKDFELPIIPPSHLDLSALHVLNKPFHHRHPLHVFTHKQNTVGDMPRSNPAPSLAITPTVLKSVNLRSI
  SKSEEVRQKEGNNTDLPYLEENAATAASVASLSPSKARPHTAKKSISRQYSAEDTLLPFLDSSVAEMGPE
  KHLEKNPNFDGKSHGDPETATSASSNLPDSNVMKEQIQMESELISENILSKNCGFSTGFQRVSASRPSDL
  GSKMIQYGASPDGTVQQGQKAPSGVREEGGKPESVDGITLQANSSTRVTDISSQYKHQRVISRHHDKVPV
  TIRHESEMSTVNSFPEKCSEQENIASGISPQSASDNSRAEETQGSMDETSLKESSPSDDSITSPLSEDSQ
  AGAEGVFVSPNKPRTTEDLFAVIHRSKRKVLGRKDSGDMSVRSKSRVPLGSSSSSANSVTSPSSNVTAGT
  SQRSPGLIYRNAKKSNTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPVLPKPPGEFTAESPQSPDDTHQ
  GTPGTEALSPLSPCSPRVNAEGFSSKNFATSASARVGRSRVPPVASSSRYSVRCRLYNTPMQAISEGETE
  NSDGSPHDDRSSQSST
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081052
• ORF Size: 4878 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081052.2, NP_001074521.1
• RefSeq Size: 5319 bp
• RefSeq ORF: 4881 bp
• Locus ID: 195727
• Cytogenetics: X 74.17 cM
• MW: 176.6 kDa