Psmd7 (NM_010817) Mouse Tagged ORF Clone

CAT#: MR227285

Psmd7 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Psmd7)

Product Images

Circular map for MR227285

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Psmd7
• Synonyms: AW107203; Mov-34; Mov34
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227285 representing NM_010817
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGAGCTGGCGGTGCAGAAGGTGGTGGTTCACCCCCTGGTGCTGCTCAGTGTGGTGGATCATTTCA
  ACCGAATTGGCAAGGTTGGAAACCAGAAGCGGGTAGTTGGTGTGCTTTTGGGATCATGGCAAAAGAAAGT
  ACTTGATGTATCCAACAGTTTTGCAGTACCTTTTGATGAAGATGACAAAGATGATTCTGTCTGGTTTTTA
  GACCATGATTATTTGGAAAACATGTATGGGATGTTCAAGAAGGTCAATGCCAGAGAAAGGATAGTTGGGT
  GGTACCACACAGGCCCCAAACTGCACAAGAATGATATCGCCATCAATGAACTCATGAAGAGATACTGCCC
  CAACTCAGTATTGGTCATTATCGACGTGAAGCCAAAGGACCTAGGACTTCCCACCGAAGCCTACATCTCA
  GTGGAGGAAGTTCATGACGATGGGACGCCAACGTCAAAAACTTTTGAGCATGTGACTAGCGAGATTGGAG
  CAGAGGAGGCGGAGGAAGTCGGAGTGGAGCACTTACTAAGAGACATCAAGGACACTACAGTGGGGACTCT
  CTCCCAGCGGATCACAAACCAGGTCCATGGCTTGAAGGGACTGAACTCCAAGCTCCTGGATATCAGGAGC
  TACCTGGAGAAGGTAGCCAGCGGCAAGCTGCCCATCAACCACCAGATCATATACCAGCTGCAGGACGTCT
  TCAACCTGCTGCCGGACGCCAGCCTGCAGGAGTTTGTCAAGGCCTTCTACCTGAAGACCAATGACCAGAT
  GGTGGTGGTGTACCTGGCCTCGCTGATCCGCTCTGTGGTCGCCTTGCATAACCTCATCAACAACAAGATT
  GCCAACCGGGATGCCGAGAAGAAGGAGGGACAGGAAAAGGAGGAGAGCAAGAAGGAGAGAAAAGACGACA
  AAGAGAAGGAGAAGAGCGACGCAGCGAAGAAAGAAGAGAAAAAGGAGAAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227285 representing NM_010817
  Red=Cloning site Green=Tags(s)
  
  MPELAVQKVVVHPLVLLSVVDHFNRIGKVGNQKRVVGVLLGSWQKKVLDVSNSFAVPFDEDDKDDSVWFL
  DHDYLENMYGMFKKVNARERIVGWYHTGPKLHKNDIAINELMKRYCPNSVLVIIDVKPKDLGLPTEAYIS
  VEEVHDDGTPTSKTFEHVTSEIGAEEAEEVGVEHLLRDIKDTTVGTLSQRITNQVHGLKGLNSKLLDIRS
  YLEKVASGKLPINHQIIYQLQDVFNLLPDASLQEFVKAFYLKTNDQMVVVYLASLIRSVVALHNLINNKI
  ANRDAEKKEGQEKEESKKERKDDKEKEKSDAAKKEEKKEKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010817
• ORF Size: 963 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010817.2, NP_034947.1
• RefSeq Size: 1616 bp
• RefSeq ORF: 966 bp
• Locus ID: 17463
• UniProt ID: P26516
• Cytogenetics: 8 54.38 cM
• MW: 36.5 kDa
• Gene Summary: Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.[UniProtKB/Swiss-Prot Function]