Adora3 (NM_009631) Mouse Tagged ORF Clone
CAT#: MR227237
- TrueORF®
Adora3 (Myc-DDK-tagged) - Mouse adenosine A3 receptor (Adora3), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_009631" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Adora3 |
Synonyms | A3AR; A3R; AA3R; ARA3; Gpcr2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR227237 representing NM_009631
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCCGACAACACCACGGAGACGGACTGGCTGAACATCACCTACATCACCATGGAGGCTGCCATCG GGCTCTGTGCCGTTGTGGGCAACATGCTGGTCATCTGGGTGGTCAAGCTGAACCCCACTCTGAGGACCAC CACGTTCTATTTCATTGTCTCCCTAGCACTGGCTGACATTGCCGTTGGGGTGCTGGTCACACCTTTGGCC ATTGCTGTCAGCCTGCAAGTCAAGATGCACTTCTATGCCTGCCTTTTCATGTCCTGTGTGCTGCTGATCT TCACCCATGCTTCCATCATGTCCTTGCTGGCCATTGCTGTAGACCGATACCTGCGGGTCAAGCTGACAGT CAGATATAGAACGGTTACCACTCAAAGAAGAATATGGCTATTCTTGGGCCTTTGCTGGCTAGTTTCCTTT CTGGTGGGGCTGACCCCCATGTTTGGCTGGAATAGAAAAGCAACCTTAGCGAGCTCTCAAAATAGCAGCA CTCTTTTGTGCCACTTCCGTTCCGTGGTCAGTTTGGATTACATGGTCTTCTTCAGCTTCGTCACCTGGAT CCTCGTCCCCCTGGTTGTCATGTGTGTCATCTACCTAGACATCTTCTACATCATCCGAAATAAGCTCAGT CAAAACCTGTCTGGCTTCAGAGAGACGCGTGCATTTTATGGACGGGAGTTCAAGACAGCTAAGTCCCTGT TTCTGGTTCTCTTCTTGTTTGCGCTGTGCTGGCTGCCTTTGTCCATCATCAATTTTGTTTCCTATTTTGA TGTAAAGATACCAGATGTCGCAATGTGCCTGGGGATCCTGTTGTCCCACGCGAACTCCATGATGAACCCT ATTGTCTACGCCTGCAAAATAAAAAAGTTCAAAGAGACCTACTTTCTGATCCTCAGAGCTCTCAGGCTCT GTCAGACCTCAGATTCTTTGGACTCAAACATGGAACAGACTACTGAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR227237 representing NM_009631
Red=Cloning site Green=Tags(s) MEADNTTETDWLNITYITMEAAIGLCAVVGNMLVIWVVKLNPTLRTTTFYFIVSLALADIAVGVLVTPLA IAVSLQVKMHFYACLFMSCVLLIFTHASIMSLLAIAVDRYLRVKLTVRYRTVTTQRRIWLFLGLCWLVSF LVGLTPMFGWNRKATLASSQNSSTLLCHFRSVVSLDYMVFFSFVTWILVPLVVMCVIYLDIFYIIRNKLS QNLSGFRETRAFYGREFKTAKSLFLVLFLFALCWLPLSIINFVSYFDVKIPDVAMCLGILLSHANSMMNP IVYACKIKKFKETYFLILRALRLCQTSDSLDSNMEQTTE SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_009631 |
ORF Size | 957 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009631.4, NP_033761.2 |
RefSeq Size | 1797 bp |
RefSeq ORF | 960 bp |
Locus ID | 11542 |
Cytogenetics | 3 46.45 cM |
MW | 36.9 kDa |
Gene Summary | This gene encodes a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death. This gene shares its 3' terminal exon with a transcript variant from overlapping GeneID:69296, which encodes an immunoglobulin domain-containing protein. [provided by RefSeq, Nov 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208110 | Adora3 (untagged) - Mouse adenosine A3 receptor (Adora3), transcript variant 1, (10ug) |
USD 300.00 |
|
MG227237 | Adora3 (tGFP-tagged) - Mouse adenosine A3 receptor (Adora3) transcript variant 1, (10ug) |
USD 500.00 |
|
MR227237L3 | Lenti ORF clone of Adora3 (Myc-DDK-tagged) - Mouse adenosine A3 receptor (Adora3), transcript variant 1 |
USD 600.00 |
|
MR227237L4 | Lenti ORF clone of Adora3 (mGFP-tagged) - Mouse adenosine A3 receptor (Adora3), transcript variant 1 |
USD 600.00 |
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