Mib1 (NM_144860) Mouse Tagged ORF Clone

CAT#: MR227236

Mib1 (Myc-DDK-tagged) - Mouse mindbomb homolog 1 (Drosophila) (Mib1)

Product Images

Circular map for MR227236

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mib1
• Synonyms: DIP-1; E430019M12Rik; Mib; mKIAA1323
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227236 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAACTCCCGCAACAACCGAGTGATGGTGGAGGGGGTCGGCGCCCGCGTAGTGCGCGGCCCGGACT
  GGAAGTGGGGCAAGCAGGACGGCGGCGAGGGCCATGTAGGCACTGTACGGAGCTTCGAGAGTCCGGAGGA
  GGTCGTGGTGGTGTGGGACAACGGCACGGCCGCCAACTACCGCTGCTCAGGGGCCTACGACCTGCGTATC
  CTGGACAGCGCGCCCACCGGCATCAAGCATGATGGAACTATGTGTGATACCTGCCGCCAGCAACCAATCA
  TTGGCATTCGATGGAAATGTGCAGAGTGCACCAATTATGATTTGTGCACTGTTTGTTATCATGGAGATAA
  ACATCACTTAAGGCATCGCTTTTATAGGATTACTACACCAGGAAGTGAACGGGTTCTGTTAGAGTCTCGT
  AGAAAATCTAAGAAGATTACAGCCAGAGGAATCTTTGCTGGTGCCAGAGTGGTGCGAGGAGTGGACTGGC
  AGTGGGAAGATCAAGATGGAGGCAATGGGCGTAGAGGAAAGGTCACGGAGATCCAGGACTGGAGTGCATC
  CAGCCCACACAGTGCAGCCTATGTTCTCTGGGATAACGGTGCTAAGAACCTTTACAGGGTTGGCTTTGAG
  GGCATGTCTGATCTCAAGTGTGTCCAGGATGCCAAAGGAGGTTCTTTCTACAGAGATCATTGCCCTGTGC
  TAGGTGAGCAGAATGGCAACAGGAATCCTGGTGGATTGCAGATTGGTGACCTGGTAAATATAGACCTTGA
  TCTAGAAATTGTCCAGTCTTTACAGCACGGTCATGGAGGATGGACCGATGGCATGTTTGAGACTTTAACT
  ACAACTGGAACCGTCTGTGGCATTGATGAAGATCATGACATTGTAGTACAGTATCCAAGTGGCAATAGAT
  GGACTTTCAATCCTGCTGTTCTCACTAAGGCAAACATTGTCCGAAGTGGGGATGCTGCACAGGGTGCAGA
  AGGAGGCACCTCACAGTTTCAAGTGGGTGATCTTGTGCAAGTTTGTTATGATCTGGAAAGAATTAAACTT
  CTCCAAAGAGGACATGGTGAATGGGCTGAAGCAATGCTTCCAACTTTAGGTAAAGTTGGCCGAGTACAAC
  AGATTTATTCGGACAGTGACTTAAAGGTGGAAGTCTGTGGAACATCTTGGACGTATAACCCTGCAGCAGT
  TTCCAAGGTGGCCCCTGCAGGATCAGCCATTAGCAATGCATCTGGTGAAAGACTCTCCCAGCTTCTGAAG
  AAATTATTTGAAACCCAAGAATCTGGTGACCTCAATGAAGAGCTGGTTAAGGCTGCTGCCAATGGAGACG
  TGGCTAAAGTGGAAGATTTGCTGAAAAGACCAGATGTGGATGTAAACGGACAGTGTGCTGGCCACACAGC
  AATGCAAGCTGCTAGTCAGAACGGACATGTTGATATTTTGAAATTACTTTTGAAGCAAAATGTTGATGTT
  GAAGCAGAGGATAAAGATGGAGATCGAGCCGTTCACCATGCAGCCTTTGGAGATGAGGGTGCTGTCATAG
  AAGTCCTACACCGAGGCAGTGCTGACCTGAATGCTCGTAATAAGCGTAGACAGACGCCTCTTCATATTGC
  TGTCAATAAAGGTCATCTTCAAGTTGTGAAGACTTTGTTGGATTTTGGTTGTCATCCCAGTCTCCAGGAT
  TCTGAAGGTGACACCCCTCTCCATGATGCCATAAGTAAGAAACGGGATGACATTCTGGCAGTTCTTCTGG
  AAGCTGGAGCAGATGTGACCATCACGAACAATAACGGGTTCAATGCCCTGCACCATGCTGCACTGAGAGG
  GAACCCCAGTGCAATGCGTGTTTTACTCTCAAAATTACCAAGACCATGGATTGTGGATGAGAAGAAAGAT
  GATGGTTATACTGCCTTGCACCTGGCTGCCCTGAATAATCATGTGGAAGTGGCTGAGCTGTTGGTGCATC
  AGGGAAATGCAAACCTGGATATCCAGAATGTGAACCAGCAAACAGCCCTACACCTTGCTGTTGAACGGCA
  ACACACCCAGATTGTTAGGCTTTTGGTCCGTGCAGGTGCTAAGCTAGACATTCAGGATAAGGATGGAGAT
  ACTCCTCTGCATGAAGCTTTGAGACATCATACTTTGTCTCAACTACGTCAGCTACAAGACATGCAAGATG
  TGGGCAAGGTAGACGCTGCCTGGGAACCTTCAAAAAACACATTAATAATGGGACTTGGTACCCAGGGGGC
  TGAGAAGAAGAGTGCAGCATCCATTGCGTGTTTCTTGGCAGCCAATGGTGCTGATCTCAGCATTCGAAAT
  AAGAAGGGTCAGTCACCACTGGATCTCTGTCCTGACCCAAGTCTCTGCAAAGCTTTGGCAAAATGTCATA
  AGGAAAAAGTCAGTGGCAAAGTGGGTTCTCGGAGTCCTTCTATGATTAGCAACGATTCTGAAACTTTAGA
  GGAATGTATGGTGTGCTCAGACATGAAGAGAGATACTCTTTTTGGTCCGTGTGGACACATTGCAACCTGT
  TCTTTGTGTTCCCCTCGTGTCAAGAAATGCCTCATTTGTAAAGAGCAGGTTCAGTCCAGGACAAAGATTG
  AAGAATGTGTGGTGTGCTCCGACAAGAAAGCAGCTGTTCTCTTTCAACCTTGTGGGCACATGTGTGCTTG
  TGAGAACTGTGCCAGCCTGATGAAAAAGTGTGTGCAGTGTCGGGCAGTGGTTGAGCGGAGGGTGCCTTTC
  ATCACGTGCTGTGGTGGCAAGAGCTCAGAAGACCCCTCTGATGAAATCTCAAGTGGGAATATCCCAGTGC
  TGCAGAAGGACAAAGACAACACCAATGTTAACGCAGATGTGCAGAAACTGCAGCAGCAGCTTCAGGACAT
  TAAGGAGCAGACCATGTGCCCTGTGTGCTTGGATCGCCTGAAGAACATGATTTTCCTCTGTGGCCATGGA
  ACATGCCAGCTCTGTGGAGACCGGATGAGTGAATGCCCAATTTGTCGCAAGGCAATTGAACGAAGGATTC
  TTTTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227236 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSNSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRI
  LDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESR
  RKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFE
  GMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLT
  TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKL
  LQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQLLK
  KLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDV
  EAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQD
  SEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKD
  DGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGD
  TPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRN
  KKGQSPLDLCPDPSLCKALAKCHKEKVSGKVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATC
  SLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPF
  ITCCGGKSSEDPSDEISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHG
  TCQLCGDRMSECPICRKAIERRILLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144860
• ORF Size: 3021 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144860.2, NP_659109.2
• RefSeq Size: 3793 bp
• RefSeq ORF: 3021 bp
• Locus ID: 225164
• UniProt ID: Q80SY4
• Cytogenetics: 18 A1
• MW: 110.1 kDa
• Gene Summary: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation (By similarity). Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.[UniProtKB/Swiss-Prot Function]