Prf1 (NM_011073) Mouse Tagged ORF Clone

CAT#: MR227213

Prf1 (Myc-DDK-tagged) - Mouse perforin 1 (pore forming protein) (Prf1)

Product Images

Circular map for MR227213

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prf1
• Synonyms: Pfn; Pfp; Prf-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227213 representing NM_011073
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGTGCCTGTTCCTCCTGGGCCTTTTCCTGCTGCTGCCACGACCTGTCCCTGCTCCCTGCTACA
  CTGCCACTCGGTCAGAATGCAAGCAGAAGCACAAGTTCGTGCCAGGTGTATGGATGGCTGGGGAAGGCAT
  GGATGTGACTACCCTCCGCCGCTCCGGCTCCTTCCCAGTGAACACACAGAGGTTCCTGAGGCCTGACCGC
  ACCTGCACCCTCTGTAAAAACTCCCTAATGAGAGACGCCACACAGCGCCTACCTGTGGCAATCACCCACT
  GGCGGCCTCACAGCTCACACTGCCAGCGTAATGTGGCCGCAGCCAAGGTCCACTCCACGGAGGGTGTGGC
  CCGGGAGGCAGCTGCTAATATCAATAACGACTGGCGTGTGGGGCTGGATGTGAACCCTAGGCCAGAGGCA
  AACATGCGCGCCTCCGTGGCTGGCTCCCACTCCAAGGTAGCCAATTTTGCAGCTGAGAAGACCTATCAGG
  ACCAGTACAACTTTAATAGCGACACAGTAGAGTGTCGCATGTACAGTTTTCGCCTGGTACAAAAACCTCC
  ACTCCACCTTGACTTCAAAAAGGCGCTCAGAGCCCTCCCCCGCAACTTTAACAGCTCCACAGAGCATGCT
  TACCACAGGCTCATCTCCTCCTATGGCACGCACTTTATCACGGCTGTGGACCTCGGTGGCCGCATCTCGG
  TCCTTACAGCCCTGCGTACCTGTCAGCTGACCCTGAATGGGCTCACAGCTGATGAGGTAGGAGACTGCCT
  GAACGTGGAGGCCCAGGTCAGCATCGGTGCCCAAGCCAGCGTCTCCAGTGAATACAAAGCTTGTGAGGAG
  AAGAAGAAACAGCACAAAATGGCCACCTCTTTCCACCAGACCTACCGTGAGCGTCACGTCGAAGTACTTG
  GTGGCCCTCTGGACTCCACGCATGATCTGCTCTTCGGGAACCAAGCTACACCAGAGCAGTTCTCAACCTG
  GACAGCCTCACTGCCCAGCAACCCTGGTCTGGTGGACTACAGCCTGGAGCCCCTGCACACATTACTGGAA
  GAACAGAACCCGAAGCGGGAGGCTCTGAGACAGGCTATCAGCCATTATATAATGAGCAGAGCCCGGTGGC
  AGAACTGTAGCAGGCCCTGCAGGTCAGGCCAGCATAAGAGTAGCCATGATTCATGCCAGTGTGAGTGCCA
  GGATTCAAAGGTCACCAACCAGGACTGCTGCCCACGACAGAGGGGCTTGGCCCATTTGGTGGTAAGCAAT
  TTCCGGGCAGAACATCTGTGGGGAGACTACACCACAGCTACTGATGCCTACCTAAAGGTCTTCTTTGGTG
  GCCAGGAGTTCAGGACCGGTGTCGTGTGGAACAATAACAATCCCCGGTGGACTGACAAGATGGACTTTGA
  GAATGTGCTCCTGTCCACAGGGGGACCCCTCAGGGTGCAGGTCTGGGATGCCGACTACGGCTGGGATGAT
  GACCTTCTTGGTTCTTGTGACAGGTCTCCCCACTCTGGTTTCCATGAGGTGACATGTGAGCTAAACCACG
  GCAGGGTGAAATTCTCCTACCATGCCAAGTGTCTGCCCCATCTCACTGGAGGGACCTGCCTGGAGTATGC
  CCCCCAGGGGCTTCTGGGAGATCCTCCAGGAAACCGCAGTGGGGCTGTGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227213 representing NM_011073
  Red=Cloning site Green=Tags(s)
  
  MATCLFLLGLFLLLPRPVPAPCYTATRSECKQKHKFVPGVWMAGEGMDVTTLRRSGSFPVNTQRFLRPDR
  TCTLCKNSLMRDATQRLPVAITHWRPHSSHCQRNVAAAKVHSTEGVAREAAANINNDWRVGLDVNPRPEA
  NMRASVAGSHSKVANFAAEKTYQDQYNFNSDTVECRMYSFRLVQKPPLHLDFKKALRALPRNFNSSTEHA
  YHRLISSYGTHFITAVDLGGRISVLTALRTCQLTLNGLTADEVGDCLNVEAQVSIGAQASVSSEYKACEE
  KKKQHKMATSFHQTYRERHVEVLGGPLDSTHDLLFGNQATPEQFSTWTASLPSNPGLVDYSLEPLHTLLE
  EQNPKREALRQAISHYIMSRARWQNCSRPCRSGQHKSSHDSCQCECQDSKVTNQDCCPRQRGLAHLVVSN
  FRAEHLWGDYTTATDAYLKVFFGGQEFRTGVVWNNNNPRWTDKMDFENVLLSTGGPLRVQVWDADYGWDD
  DLLGSCDRSPHSGFHEVTCELNHGRVKFSYHAKCLPHLTGGTCLEYAPQGLLGDPPGNRSGAVW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011073
• ORF Size: 1662 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011073.3, NP_035203.3
• RefSeq Size: 2054 bp
• RefSeq ORF: 1665 bp
• Locus ID: 18646
• UniProt ID: P10820
• Cytogenetics: 10 32.18 cM
• MW: 62.5 kDa
• Gene Summary: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.[UniProtKB/Swiss-Prot Function]