Kl (NM_013823) Mouse Tagged ORF Clone

CAT#: MR227101

Kl (Myc-DDK-tagged) - Mouse klotho (Kl)

Product Images

Circular map for MR227101

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kl
• Synonyms: alpha-kl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227101 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAGCCCGCGCCCCTCCTCGCCGCCCGCCGCGGCTGGTGCTGCTCCGTTTGCTGTTGCTGCATCTGC
  TGCTGCTCGCCCTGCGCGCCCGCTGCCTGAGCGCTGAGCCGGGTCAGGGCGCGCAGACCTGGGCTCGCTT
  CGCGCGCGCTCCTGCCCCAGAGGCCGCTGGCCTCCTCCACGACACCTTCCCCGACGGTTTCCTCTGGGCG
  GTAGGCAGCGCCGCCTATCAGACCGAGGGCGGCTGGCGACAGCACGGCAAAGGCGCGTCCATCTGGGACA
  CTTTCACCCATCACTCTGGGGCGGCCCCGTCCGACTCCCCGATCGTCGTGGCGCCGTCGGGTGCCCCGTC
  GCCTCCCCTGTCCTCCACTGGAGATGTGGCCAGCGATAGTTACAACAACGTCTACCGCGACACAGAGGGG
  CTGCGCGAACTGGGGGTCACCCACTACCGCTTCTCCATATCGTGGGCGCGGGTGCTCCCCAATGGCACCG
  CGGGCACTCCCAACCGCGAGGGGCTGCGCTACTACCGGCGGCTGCTGGAGCGGCTGCGGGAGCTGGGCGT
  GCAGCCGGTGGTTACCCTGTACCATTGGGACCTGCCACAGCGCCTGCAGGACACCTATGGCGGATGGGCC
  AATCGCGCCCTGGCCGACCATTTCAGGGATTATGCCGAGCTCTGCTTCCGCCACTTCGGTGGTCAGGTCA
  AGTACTGGATCACCATTGACAACCCCTACGTGGTGGCCTGGCACGGGTATGCCACCGGGCGCCTGGCCCC
  GGGCGTGAGGGGCAGCTCCAGGCTCGGGTACCTGGTTGCCCACAACCTACTTTTGGCTCATGCCAAAGTC
  TGGCATCTCTACAACACCTCTTTCCGCCCCACACAGGGAGGCCGGGTGTCTATCGCCTTAAGCTCCCATT
  GGATCAATCCTCGAAGAATGACTGACTATAATATCAGAGAATGCCAGAAGTCTCTTGACTTTGTGCTAGG
  CTGGTTTGCCAAACCCATATTTATTGATGGCGACTACCCAGAGAGTATGAAGAACAACCTCTCGTCTCTT
  CTGCCTGATTTTACTGAATCTGAGAAGAGGCTCATCAGAGGAACTGCTGACTTTTTTGCTCTCTCCTTCG
  GACCAACCTTGAGCTTTCAGCTATTGGACCCTAACATGAAGTTCCGCCAATTGGAGTCTCCCAACCTGAG
  GCAGCTTCTGTCTTGGATAGATCTGGAATATAACCACCCTCCAATATTTATTGTGGAAAATGGCTGGTTT
  GTCTCGGGAACCACCAAAAGGGATGATGCCAAATATATGTATTATCTCAAGAAGTTCATAATGGAAACCT
  TAAAAGCAATCAGACTGGATGGGGTCGACGTCATTGGGTACACCGCGTGGTCGCTCATGGACGGTTTCGA
  GTGGCATAGGGGCTACAGCATCCGGCGAGGACTCTTCTACGTTGACTTTCTGAGTCAGGACAAGGAGCTG
  TTGCCAAAGTCTTCGGCCTTGTTCTACCAAAAGCTGATAGAGGACAATGGCTTTCCTCCTTTACCTGAAA
  ACCAGCCCCTTGAAGGGACATTTCCCTGTGACTTTGCTTGGGGAGTTGTTGACAACTACGTTCAAGTGGA
  CACTACTCTCTCTCAGTTTACTGACCCGAATGTCTATCTGTGGGATGTGCATCACAGTAAGAGGCTTATT
  AAAGTAGACGGGGTTGTAGCCAAGAAGAGAAAACCTTACTGTGTTGATTTCTCTGCCATCCGGCCTCAGA
  TAACCTTACTTCGAGAAATGCGGGTCACCCACTTTCGCTTCTCCCTGGACTGGGCCCTGATCTTGCCTCT
  GGGTAACCAGACCCAAGTGAACCACACGGTTCTGCACTTCTACCGCTGCATGATCAGCGAGCTGGTGCAC
  GCCAACATCACTCCAGTGGTGGCCCTGTGGCAGCCAGCAGCCCCGCACCAAGGCCTGCCACATGCCCTTG
  CAAAACATGGGGCCTGGGAGAACCCGCACACTGCTCTGGCGTTTGCAGACTACGCAAACCTGTGTTTTAA
  AGAGTTGGGTCACTGGGTCAATCTCTGGATCACCATGAACGAGCCAAACACACGGAACATGACCTATCGT
  GCCGGGCACCACCTCCTGAGAGCCCATGCCTTGGCTTGGCATCTGTACGATGACAAGTTTAGGGCGGCTC
  AGAAAGGCAAAATATCCATCGCCTTGCAGGCTGACTGGATAGAACCGGCCTGCCCTTTCTCTCAAAATGA
  CAAAGAAGTGGCCGAGAGAGTTTTGGAATTTGATATAGGCTGGCTGGCAGAGCCTATTTTTGGTTCCGGA
  GATTATCCACGTGTGATGAGGGACTGGCTGAACCAAAAAAACAATTTTCTTTTGCCCTATTTCACCGAAG
  ATGAAAAAAAGCTAGTCCGGGGTTCCTTTGACTTCCTGGCGGTGAGTCATTACACCACCATTCTGGTAGA
  CTGGGAAAAGGAGGATCCGATGAAATACAACGATTACTTGGAGGTACAGGAGATGACTGACATCACATGG
  CTCAACTCTCCCAGTCAGGTGGCAGTGGTGCCTTGGGGGCTGCGCAAAGTGCTCAACTGGCTAAGGTTCA
  AGTACGGAGACCTCCCGATGTATGTGACAGCCAATGGAATCGATGATGACCCCCACGCCGAGCAAGACTC
  ACTGAGGATCTATTATATTAAGAATTATGTGAATGAGGCTCTGAAAGCCTACGTGTTGGACGACATCAAC
  CTTTGTGGCTACTTTGCGTATTCACTTAGTGATCGCTCAGCTCCCAAGTCTGGCTTTTATCGATATGCTG
  CGAATCAGTTTGAGCCCAAACCATCTATGAAACATTACAGGAAAATTATTGACAGCAATGGCTTCCTGGG
  TTCTGGAACACTGGGAAGGTTTTGTCCAGAAGAATACACTGTGTGCACCGAATGTGGATTTTTTCAAACC
  CGGAAGTCTTTGCTGGTCTTCATCTCGTTTCTTGTTTTTACTTTTATTATTTCTCTTGCTCTCATTTTTC
  ACTACTCCAAGAAAGGCCAGAGAAGTTATAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227101 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLARAPPRRPPRLVLLRLLLLHLLLLALRARCLSAEPGQGAQTWARFARAPAPEAAGLLHDTFPDGFLWA
  VGSAAYQTEGGWRQHGKGASIWDTFTHHSGAAPSDSPIVVAPSGAPSPPLSSTGDVASDSYNNVYRDTEG
  LRELGVTHYRFSISWARVLPNGTAGTPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDTYGGWA
  NRALADHFRDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGVRGSSRLGYLVAHNLLLAHAKV
  WHLYNTSFRPTQGGRVSIALSSHWINPRRMTDYNIRECQKSLDFVLGWFAKPIFIDGDYPESMKNNLSSL
  LPDFTESEKRLIRGTADFFALSFGPTLSFQLLDPNMKFRQLESPNLRQLLSWIDLEYNHPPIFIVENGWF
  VSGTTKRDDAKYMYYLKKFIMETLKAIRLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKEL
  LPKSSALFYQKLIEDNGFPPLPENQPLEGTFPCDFAWGVVDNYVQVDTTLSQFTDPNVYLWDVHHSKRLI
  KVDGVVAKKRKPYCVDFSAIRPQITLLREMRVTHFRFSLDWALILPLGNQTQVNHTVLHFYRCMISELVH
  ANITPVVALWQPAAPHQGLPHALAKHGAWENPHTALAFADYANLCFKELGHWVNLWITMNEPNTRNMTYR
  AGHHLLRAHALAWHLYDDKFRAAQKGKISIALQADWIEPACPFSQNDKEVAERVLEFDIGWLAEPIFGSG
  DYPRVMRDWLNQKNNFLLPYFTEDEKKLVRGSFDFLAVSHYTTILVDWEKEDPMKYNDYLEVQEMTDITW
  LNSPSQVAVVPWGLRKVLNWLRFKYGDLPMYVTANGIDDDPHAEQDSLRIYYIKNYVNEALKAYVLDDIN
  LCGYFAYSLSDRSAPKSGFYRYAANQFEPKPSMKHYRKIIDSNGFLGSGTLGRFCPEEYTVCTECGFFQT
  RKSLLVFISFLVFTFIISLALIFHYSKKGQRSYK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013823
• ORF Size: 3045 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013823.2, NP_038851.2
• RefSeq Size: 5124 bp
• RefSeq ORF: 3045 bp
• Locus ID: 16591
• UniProt ID: O35082
• Cytogenetics: 5 G3
• MW: 116.4 kDa
• Gene Summary: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.[UniProtKB/Swiss-Prot Function]