Tec (NM_001113464) Mouse Tagged ORF Clone

CAT#: MR227097

Tec (Myc-DDK-tagged) - Mouse tec protein tyrosine kinase (Tec), transcript variant 2

Product Images

Circular map for MR227097

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tec
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227097 representing NM_001113464
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTCAACACTATCCTAGAAGAGATTCTTATTAAAAGGTCCCAGCAGAAAAAGAAGACATCACCCT
  TAAACTACAAAGAGAGACTTTTTGTACTTACAAAATCCGTGTTGAGCTACTATGAGGGTCGAGCGGAGAA
  GAAATACAGAAAGGGCGTCATTGATATTTCCAAAATCAAGTGTGTGGAGATAGTGAAGAACGATGATGGT
  GTCATTCCCTGTCAAAATAAATTTCCATTCCAGGTTGTTCATGATGCTAATACACTTTATATTTTTGCAC
  CTAGTCCACAAAGCAGGGACCGATGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAATATCAT
  GATTAAATACCATCCTAAATTCTGGGCAGATGGGAGTTACCAGTGTTGTAGACAAACAGAAAAACTAGCA
  CCCGGATGTGAGAAGTACAATCTTTTTGAGAGTAGTATAAGAAAGACCCTGCCTCCCGCGCCAGAAATAA
  AGAAGAGAAGGCCTCCTCCACCAATTCCCCCAGAGGAAGAAAATACTGAAGAAATCGTTGTAGCGATGTA
  TGACTTCCAAGCGACGGAAGCACATGACCTCAGGTTAGAGAGAGGCCAAGAGTATATCATCCTGGAAAAG
  AATGACCTCCATTGGTGGAGAGCGAGAGATAAGTATGGGAGTGAAGGATATATCCCAAGTAATTACGTCA
  CAGGGAAGAAATCCAACAACTTAGATCAATATGAGTGGTACTGCAGAAATACCAACAGAAGCAAAGCAGA
  ACAGCTCCTCAGAACGGAAGATAAAGAAGGTGGTTTTATGGTGAGAGACTCCAGTCAACCAGGCTTGTAC
  ACTGTCTCCCTTTACACAAAGTTTGGGGGAGAAGGCTCATCAGGTTTCAGGCATTATCACATAAAGGAAA
  CAGCAACATCCCCAAAGAAGTATTACCTGGCAGAGAAGCATGCTTTCGGGTCCATTCCTGAGATCATTGA
  ATATCACAAGCACAATGCGGCAGGGCTTGTCACCAGGCTGCGGTACCCGGTCAGTACAAAGGGGAAGAAC
  GCTCCCACTACTGCCGGCTTCAGCTATGATAAGTGGGAGATTAACCCATCAGAGCTGACCTTTATGAGAG
  AGTTGGGGAGCGGACTGTTTGGAGTGGTGAGGCTTGGCAAGTGGCGGGCCCAGTACAAAGTGGCCATCAA
  AGCTATCCGGGAAGGCGCCATGTGTGAAGAGGATTTCATAGAGGAAGCTAAAGTCATGATGAAGCTGACA
  CACCCCAAGCTGGTACAGCTCTATGGTGTATGCACCCAGCAGAAGCCCATCTACATCGTTACCGAGTTCA
  TGGAACGGGGCTGCCTTCTGAATTTCCTCCGGCAGAGACAAGGCCATTTCAGCAGAGACATGCTGCTAAG
  CATGTGTCAAGATGTCTGTGAAGGGATGGAGTACCTGGAGAGAAACAGCTTCATCCACAGAGACCTGGCT
  GCCAGAAATTGTCTAGTGAATGAAGCAGGAGTTGTCAAAGTATCTGATTTTGGAATGGCCAGGTACGTTC
  TGGATGATCAGTACACAAGTTCTTCTGGCGCCAAGTTCCCTGTGAAGTGGTGTCCCCCAGAAGTGTTTAA
  TTACAGCCGCTTTAGCAGCAAGTCAGACGTCTGGTCGTTTGGTGTGCTAATGTGGGAAATATTCACAGAA
  GGCAGGATGCCCTTTGAGAAGAACACCAATTACGAAGTGGTAACCATGGTGACTCGTGGCCACCGCCTCC
  ACCGGCCAAAGCTGGCTTCCAAATATTTGTATGAGGTGATGCTGAGATGCTGGCAAGAGAGACCAGAGGG
  AAGGCCTTCCTTTGAAGACTTGCTGCGTACGATAGATGAACTAGTTGAATGTGAAGAAACTTTTGGAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227097 representing NM_001113464
  Red=Cloning site Green=Tags(s)
  
  MNFNTILEEILIKRSQQKKKTSPLNYKERLFVLTKSVLSYYEGRAEKKYRKGVIDISKIKCVEIVKNDDG
  VIPCQNKFPFQVVHDANTLYIFAPSPQSRDRWVKKLKEEIKNNNNIMIKYHPKFWADGSYQCCRQTEKLA
  PGCEKYNLFESSIRKTLPPAPEIKKRRPPPPIPPEEENTEEIVVAMYDFQATEAHDLRLERGQEYIILEK
  NDLHWWRARDKYGSEGYIPSNYVTGKKSNNLDQYEWYCRNTNRSKAEQLLRTEDKEGGFMVRDSSQPGLY
  TVSLYTKFGGEGSSGFRHYHIKETATSPKKYYLAEKHAFGSIPEIIEYHKHNAAGLVTRLRYPVSTKGKN
  APTTAGFSYDKWEINPSELTFMRELGSGLFGVVRLGKWRAQYKVAIKAIREGAMCEEDFIEEAKVMMKLT
  HPKLVQLYGVCTQQKPIYIVTEFMERGCLLNFLRQRQGHFSRDMLLSMCQDVCEGMEYLERNSFIHRDLA
  ARNCLVNEAGVVKVSDFGMARYVLDDQYTSSSGAKFPVKWCPPEVFNYSRFSSKSDVWSFGVLMWEIFTE
  GRMPFEKNTNYEVVTMVTRGHRLHRPKLASKYLYEVMLRCWQERPEGRPSFEDLLRTIDELVECEETFGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113464
• ORF Size: 1890 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113464.2, NP_001106935.1
• RefSeq Size: 2574 bp
• RefSeq ORF: 1893 bp
• Locus ID: 21682
• UniProt ID: P24604
• Cytogenetics: 5 38.44 cM
• MW: 73.5 kDa
• Gene Summary: Non-receptor tyrosine kinase that contributes to signaling from many receptors and participates as a signal transducer in multiple downstream pathways, including regulation of the actin cytoskeleton. Plays a redundant role to ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Required for TCR-dependent IL2 gene induction. Phosphorylates DOK1, one CD28-specific substrate, and contributes to CD28-signaling. Mediates signals that negatively regulate IL2RA expression induced by TCR cross-linking. Plays a redundant role to BTK in BCR-signaling for B-cell development and activation, especially by phosphorylating STAP1, a BCR-signaling protein. Required in mast cells for efficient cytokine production. Involved in both growth and differentiation mechanisms of myeloid cells through activation by the granulocyte colony-stimulating factor CSF3, a critical cytokine to promoting the growth, differentiation, and functional activation of myeloid cells. Participates in platelet signaling downstream of integrin activation. Cooperates with JAK2 through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. GRB10, a negative modifier of the FOS activation pathway, is another substrate of TEC. TEC is involved in G protein-coupled receptor- and integrin-mediated signalings in blood platelets. Plays a role in hepatocyte proliferation and liver regeneration and is involved in HGF-induced ERK signaling pathway. TEC regulates also FGF2 unconventional secretion (endoplasmic reticulum (ER)/Golgi-independent mechanism) under various physiological conditions through phosphorylation of FGF2 'Tyr-82'. May also be involved in the regulation of osteoclast differentiation.[UniProtKB/Swiss-Prot Function]