Plxna1 (NM_008881) Mouse Tagged ORF Clone

CAT#: MR226796

Plxna1 (Myc-DDK-tagged) - Mouse plexin A1 (Plxna1)

Product Images

Circular map for MR226796

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plxna1
• Synonyms: 2600013D04Rik; mKIAA4053; NOV; PlexA1; Plxn1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226796 representing NM_008881
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACTGCCACCTCTGAGCTCTCGGACACTGCTGCTGCTGCTCTTGCTGCTGCTGAGGGGTGTGTGGA
  TAGCCATCAGCTCGCCCCCAGCTGGTTTGGGTCCACAACCTGCCTTCCGCACCTTTGTGGCCAGTGACTG
  GGGCCTCACTCACCTGGTGGTTCACGAACAGACAGGAGAGGTATATGTTGGCGCTGTGAACCGTATTTAC
  AAGCTGTCGGGGAACCTAACCCTGCTTCGGGCCCATGTGACAGGCCCTGTAGAAGACAATGAGAAATGCT
  ACCCACCCCCCAGTGTACAGTCCTGCCCACATGGTCTGGGCAGCACAGACAACGTCAACAAGCTTCTACT
  GCTGGACTATGCAGCCAACCGCCTGTTGGCCTGTGGCAGTGCCTCACAGGGCATCTGCCAGTTCCTGAGG
  TTGGACGATCTCTTCAAGCTGGGCGAGCCGCACCACCGCAAGGAACACTACCTGTCCAGTGTTCGAGAGG
  CTGGCAGCATGGCGGGAGTGCTCATTGCTGGGCCACCCGGCCAGGGCCAGGCCAAGCTCTTCGTGGGCAC
  ACCCATCGATGGCAAATCAGAATACTTCCCCACTCTGTCCAGTCGCCGGCTCATGGCCAACGAGGAAGAT
  GCTGACATGTTTGGCTTTGTGTACCAGGATGAGTTTGTGTCATCTCAGCTCAAGATCCCTTCAGACACAT
  TGTCCAAGTTCCCAGCCTTTGACATCTACTATGTGTACAGTTTCCGCAGCGAGCAGTTCGTATACTACCT
  CACCCTGCAGCTAGACACGCAGCTTACCTCGCCCGACGCCGCTGGCGAGCACTTCTTCACCTCCAAGATT
  GTGCGGCTGTGCGTGAATGACCCCAAGTTCTACTCTTACGTGGAGTTCCCCATTGGCTGCGAGCAGGCTG
  GCGTGGAGTATCGGCTGGTGCAGGACGCCTACCTGAGCCGGCCGGGACAGGCCCTGGCCAAACAGCTGGG
  CCTGGCTGAGGATGAGGAGGTGTTGTTCACTGTGTTCGCACAGGGCCAGAAGAACCGCGTGAAGCCACCC
  AAGGAGTCGGCGCTGTGCCTGTTCACACTGAGGGCCATCAAGGAGAAAATCAAGGAGCGCATCCAATCCT
  GCTACCGTGGAGAAGGAAAGCTCTCCCTGCCTTGGCTGCTCAACAAGGAGCTGGGCTGCATCAACTCGCC
  CCTGCAGATTGATGACGACTTCTGCGGGCAGGATTTCAACCAGCCACTGGGTGGCACGGTCACCATCGAG
  GGCACACCCCTTTTTGTCGACAAGGAAGATGGCCTGACTGCTGTGGCTGCCTATGACTACCAAGGCCGCA
  CCGTGGTTTTTGCTGGCACTCGCAGCGGCCGCATCCGCAAGATTCTGGTGGACCTTGCAAACCCCAGCGG
  CAGGCCGGCCCTGGCTTATGAAAGTGTGGTGGCTCAGGAGGGCAACCCTATCCTGCGTGACCTGGTCCTC
  AGCCCCAACCGCCAGTACCTCTATGCTATGACGGAGAAGCAGGTGACGCAGGTGCCTGTGGAAAGCTGTG
  TCCAGTATACTTCCTGTGAGCTGTGCCTGGGGTCTCGGGACCCCCACTGTGGCTGGTGTGTCCTGCACAG
  CATCTGCTCTAGGCAGGATGCCTGTGAGCGGGCAGAAGAGCCCCAGCGTTTTGCCTCTGACCTGCTGCAG
  TGTGTGCAGTTAACTGTCCAGCCGCGCAATGTGTCAGTCACCATGTCCCAGGTGCCGCTCGTGCTGCAGG
  CTTGGAATGTGCCTGACCTCTCAGCTGGTGTCAACTGCTCCTTTGAGGACTTCACGGAGACTGAGAGCAT
  CCTGGAAGATGGCCGCATCCATTGCCACTCACCTTCTGCCCGGGAAGTGGCGCCCATCACACAGGGTCAG
  GGAGACCAGCGGGTGGTGAAGCTCTACCTCAAGTCCAAGGAGACAGGGAAGAAGTTTGCATCTGTGGACT
  TTGTCTTCTATAACTGCAGTGTTCACCAGTCCTGCCTGGCATGTGTCAACGGCTCCTTCCCCTGCCATTG
  GTGCAAATACCGCCATGTTTGCACCAACAATGCGGCTGACTGCGCCTTTCTGGAAGGCCGAGTCAACATG
  TCTGAGGACTGCCCACAGATCCTGCCTTCTACCCACATCTATGTGCCAGTGGGTGTGGTGAAGCCCATCA
  CCCTGGCTGCACGGAACCTGCCGCAGCCACAGTCGGGTCAGCGAGGGTACGAGTGCCTTTTCCACATCCC
  AGGCAGCCCGGCCCGAGTCACTGCACTGCGCTTCAACAGCTCCAGCCTGCAGTGCCAGAACTCCTCGTAC
  TCCTATGAGGGGAATGATGTCAGCGATCTGCCTGTGAACCTGTCGGTTGTGTGGAATGGCAACTTTGTCA
  TTGACAACCCCCAGAACATCCAGGCCCACCTCTACAAGTGCCCAGCCTTGCGCCAGAGTTGTGGCCTCTG
  CCTCAAGGCAGACCCACGCTTTGAGTGCGGCTGGTGCGTAGCTGAGCGCCGCTGTTCCCTTCGTCACCAC
  TGCCCAGCTGACTCACCAGCCTCCTGGATGCATGCCCACCATGGCAGCAGCCGCTGCACAGACCCCAAGA
  TTCTCAAGCTGTCCCCAGAGACAGGCCCTCGGCAGGGAGGGACTCGACTAACCATCACCGGTGAGAATCT
  GGGCCTCCGGTTTGAGGATGTGCGGCTAGGCGTGCACGTGGGCAAGGTGCTTTGCAGCCCTGTGGAGAGC
  GAGTACATCAGCGCAGAACAGATTGTCTGCGAGATTGGAGATGCCAGCACGCTGCGGGCCCATGATGCCC
  TGGTGGAGGTGTGTGTGCGGGACTGCTCCCTCCACTACCGAGCGCTGTCCCCTAAACGCTTCACTTTTGT
  GACACCGACCTTCTACCGTGTGAGCCCCTCCCGTGGGCCTCTGTCTGGAGGTACCTGGATTGGCATCGAA
  GGGAGCCATCTGAATGCTGGCAGTGATGTAGCTGTGTCCATTGGCGGTCGGCCCTGCTCCTTCTCCTGGA
  GAAACTCACGAGAGATCCGCTGCCTGACACCGCCCGGGCACACTCCTGGCAGCGCCCCTATTGTTATCAA
  CATCAATCGTGCCCAGCTCTCCAACCCCGAGGTGAAGTACAACTATACTGAAGACCCTACTATCCTGAGG
  ATCGACCCTGAGTGGAGCATCAATAGTGGTGGGACCCTCCTAACAGTCACGGGCACCAACCTGGCCACTG
  TCCGTGAACCTCGAATCCGCGCCAAGTATGGAGGCATTGAGAGGGAAAATAGCTGCATGGTGTACAATGA
  CACCACCATGGTGTGCCGGGCGCCGTCTATAGACAACCCAAAGCGCAGCCCGCCAGAACTGGGAGAGCGG
  CCAGATGAGATTGGTTTCATCATGGACAATGTGCGCACGCTGCTGGTACTCAACAGCTCCTCCTTCCTCT
  ACTACCCGGACCCTGTACTGGAGCCACTTAGCCCTACTGGGCTCTTGGAGCTGAAGCCCAGCTCCCCGCT
  CATCCTCAAGGGGCGGAATCTCCTGCCACCTGCTCCTGGCAATTCCCGGCTCAACTACACGGTGCTCATC
  GGCTCCACACCTTGTATCCTCACCGTGTCTGAGACACAGCTGCTGTGTGAAGCACCCAACCTCACGGGGC
  AGCACAAAGTCACGGTGCGGGCCGGGGGCTTCGAGTTCTCACCTGGGATGCTGCAGGTGTATTCGGACAG
  CCTGCTGACACTGCCGGCCATTGTGGGTATCGGTGGTGGGGGTGGCCTCTTGCTGCTGGTCATCGTGGCT
  GTGCTCATCGCCTACAAACGCAAATCCCGGGATGCTGACCGTACCCTCAAGCGGCTGCAGCTCCAGATGG
  ACAACCTGGAGTCACGGGTGGCCCTCGAGTGCAAGGAAGCTTTTGCAGAGCTACAGACGGACATCCATGA
  GCTGACCAGTGACCTGGATGGTGCTGGCATCCCCTTCCTTGACTACCGCACATATGCCATGCGAGTGCTG
  TTTCCTGGGATTGAGGACCATCCTGTACTGAAGGAGATGGAGGTGCAGGCCAATGTGGAGAAGTCGCTGA
  CACTGTTCGGACAGCTGTTGACCAAGAAGCATTTCCTGCTAACCTTCATCCGCACACTAGAGGCCCAGCG
  TAGCTTCTCCATGCGTGACCGTGGGAACGTGGCCTCACTCATCATGACAGCCCTGCAGGGTGAGATGGAG
  TATGCCACAGGAGTGCTAAAGCAGTTGCTGTCTGACCTCATCGAGAAAAACCTAGAAAGCAAGAACCACC
  CCAAGCTCCTGTTACGGAGGACCGAATCAGTGGCAGAGAAGATGTTGACCAACTGGTTCACCTTCCTTTT
  GTATAAGTTCCTCAAGGAGTGTGCTGGGGAGCCGCTCTTTATGCTGTACTGTGCCATCAAGCAGCAGATG
  GAGAAGGGCCCTATTGACGCCATCACAGGGGAGGCCCGCTACTCTCTGAGCGAAGACAAGCTCATTCGGC
  AGCAGATTGACTACAAGACCCTGACCCTGAACTGCGTGAACCCTGAACATGAGAATGCGCCCGAGGTTCC
  TGTGAAGGGGCTGAACTGTGACACCGTGACCCAGGTCAAGGAGAAGCTCCTGGATGCTGTGTACAAGGGT
  GTGCCTTACTCACAGCGGCCGAAGGCCGGGGACATGGACCTGGAGTGGCGCCAGGGTCGCATGGCACGCA
  TCATCCTTCAGGACGAGGACGTCACCACTAAAATTGACAATGACTGGAAGAGGCTGAACACACTAGCTCA
  CTATCAGGTGACGGATGGGTCATCGGTGGCACTAGTACCCAAGCAGACATCGGCCTACAACATCTCCAAC
  TCCTCTACCTTCACTAAGTCCCTCAGCAGATACGAGAGCATGCTGCGTACAGCCAGTAGCCCCGACAGCC
  TACGCTCACGAACACCCATGATCACGCCAGACCTGGAAAGTGGAACAAAACTGTGGCACCTGGTGAAGAA
  CCACGACCACCTGGACCAACGTGAAGGCGACCGTGGCAGCAAGATGGTCTCTGAGATCTACCTAACCAGG
  CTGCTGGCCACCAAGGGAACACTACAAAAGTTCGTGGATGACCTGTTCGAGACCATCTTCAGCACGGCGC
  ACCGTGGCTCAGCCCTACCTCTGGCCATCAAGTACATGTTTGACTTCTTAGATGAGCAGGCCGACAAGCA
  TCAGATCCACGACTCAGATGTGCGCCATACCTGGAAGAGCAATTGCCTGCCTCTGCGCTTCTGGGTGAAT
  GTGATTAAGAACCCTCAGTTCGTGTTCGACATCCACAAGAACAGCATAACAGATGCCTGCCTCTCAGTGG
  TAGCGCAGACCTTCATGGACTCCTGCTCTACATCAGAGCACAAGCTGGGCAAAGACTCACCCTCCAACAA
  GTTGCTCTATGCCAAGGACATCCCCAACTACAAGAGCTGGGTTGAAAGGTACTATGCTGACATTGCCAAG
  ATGCCTGCAATCAGTGACCAAGACATGAGCGCCTACCTGGCAGAGCAGTCCCGGCTGCATCTCAGCCAGT
  TCAACAGCATGAGTGCCCTGCACGAGATCTACTCCTACATCGCCAAGTACAAGGACGAGATTCTGGTGGC
  TCTGGAGAAAGATGAACAGGCTCGAAGGCAGCGGCTTCGAAGCAAACTGGAGCAGGTGGTGGACACAATG
  GCCCTGAGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226796 representing NM_008881
  Red=Cloning site Green=Tags(s)
  
  MPLPPLSSRTLLLLLLLLLRGVWIAISSPPAGLGPQPAFRTFVASDWGLTHLVVHEQTGEVYVGAVNRIY
  KLSGNLTLLRAHVTGPVEDNEKCYPPPSVQSCPHGLGSTDNVNKLLLLDYAANRLLACGSASQGICQFLR
  LDDLFKLGEPHHRKEHYLSSVREAGSMAGVLIAGPPGQGQAKLFVGTPIDGKSEYFPTLSSRRLMANEED
  ADMFGFVYQDEFVSSQLKIPSDTLSKFPAFDIYYVYSFRSEQFVYYLTLQLDTQLTSPDAAGEHFFTSKI
  VRLCVNDPKFYSYVEFPIGCEQAGVEYRLVQDAYLSRPGQALAKQLGLAEDEEVLFTVFAQGQKNRVKPP
  KESALCLFTLRAIKEKIKERIQSCYRGEGKLSLPWLLNKELGCINSPLQIDDDFCGQDFNQPLGGTVTIE
  GTPLFVDKEDGLTAVAAYDYQGRTVVFAGTRSGRIRKILVDLANPSGRPALAYESVVAQEGNPILRDLVL
  SPNRQYLYAMTEKQVTQVPVESCVQYTSCELCLGSRDPHCGWCVLHSICSRQDACERAEEPQRFASDLLQ
  CVQLTVQPRNVSVTMSQVPLVLQAWNVPDLSAGVNCSFEDFTETESILEDGRIHCHSPSAREVAPITQGQ
  GDQRVVKLYLKSKETGKKFASVDFVFYNCSVHQSCLACVNGSFPCHWCKYRHVCTNNAADCAFLEGRVNM
  SEDCPQILPSTHIYVPVGVVKPITLAARNLPQPQSGQRGYECLFHIPGSPARVTALRFNSSSLQCQNSSY
  SYEGNDVSDLPVNLSVVWNGNFVIDNPQNIQAHLYKCPALRQSCGLCLKADPRFECGWCVAERRCSLRHH
  CPADSPASWMHAHHGSSRCTDPKILKLSPETGPRQGGTRLTITGENLGLRFEDVRLGVHVGKVLCSPVES
  EYISAEQIVCEIGDASTLRAHDALVEVCVRDCSLHYRALSPKRFTFVTPTFYRVSPSRGPLSGGTWIGIE
  GSHLNAGSDVAVSIGGRPCSFSWRNSREIRCLTPPGHTPGSAPIVININRAQLSNPEVKYNYTEDPTILR
  IDPEWSINSGGTLLTVTGTNLATVREPRIRAKYGGIERENSCMVYNDTTMVCRAPSIDNPKRSPPELGER
  PDEIGFIMDNVRTLLVLNSSSFLYYPDPVLEPLSPTGLLELKPSSPLILKGRNLLPPAPGNSRLNYTVLI
  GSTPCILTVSETQLLCEAPNLTGQHKVTVRAGGFEFSPGMLQVYSDSLLTLPAIVGIGGGGGLLLLVIVA
  VLIAYKRKSRDADRTLKRLQLQMDNLESRVALECKEAFAELQTDIHELTSDLDGAGIPFLDYRTYAMRVL
  FPGIEDHPVLKEMEVQANVEKSLTLFGQLLTKKHFLLTFIRTLEAQRSFSMRDRGNVASLIMTALQGEME
  YATGVLKQLLSDLIEKNLESKNHPKLLLRRTESVAEKMLTNWFTFLLYKFLKECAGEPLFMLYCAIKQQM
  EKGPIDAITGEARYSLSEDKLIRQQIDYKTLTLNCVNPEHENAPEVPVKGLNCDTVTQVKEKLLDAVYKG
  VPYSQRPKAGDMDLEWRQGRMARIILQDEDVTTKIDNDWKRLNTLAHYQVTDGSSVALVPKQTSAYNISN
  SSTFTKSLSRYESMLRTASSPDSLRSRTPMITPDLESGTKLWHLVKNHDHLDQREGDRGSKMVSEIYLTR
  LLATKGTLQKFVDDLFETIFSTAHRGSALPLAIKYMFDFLDEQADKHQIHDSDVRHTWKSNCLPLRFWVN
  VIKNPQFVFDIHKNSITDACLSVVAQTFMDSCSTSEHKLGKDSPSNKLLYAKDIPNYKSWVERYYADIAK
  MPAISDQDMSAYLAEQSRLHLSQFNSMSALHEIYSYIAKYKDEILVALEKDEQARRQRLRSKLEQVVDTM
  ALSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008881
• ORF Size: 5682 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008881.2, NP_032907.1
• RefSeq Size: 9045 bp
• RefSeq ORF: 5685 bp
• Locus ID: 18844
• UniProt ID: P70206
• Cytogenetics: 6 D1
• MW: 211.5 kDa
• Gene Summary: Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.[UniProtKB/Swiss-Prot Function]