Htra3 (NM_030127) Mouse Tagged ORF Clone

CAT#: MR226669

Htra3 (Myc-DDK-tagged) - Mouse HtrA serine peptidase 3 (Htra3), transcript variant 1

Product Images

Circular map for MR226669

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Htra3
• Synonyms: 2210021K23Rik; 9530081K03Rik; Prsp; Tasp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226669 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCGCGCGCGCTGCTCCCCGCCACGCTGGCCATTCTGGCCACGCTGGCTGTGTTGGCTCTGGCCC
  GGGAGCCCCCAGCGGCTCCGTGTCCTGCGCGCTGCGACGTGTCGCGCTGTCCGAGCCCTCGCTGCCCTGG
  GGGCTATGTGCCTGACCTCTGCAACTGCTGCCTGGTGTGCGCTGCCAGCGAGGGCGAGCCCTGCGGCCGC
  CCCCTGGACTCTCCGTGCGGGGACAGTCTGGAGTGCGTGCGCGGCGTGTGCCGCTGCCGTTGGACCCACA
  CTGTGTGTGGCACAGACGGGCATACTTATGCCGACGTGTGCGCGCTGCAGGCCGCCAGCCGTCGTGCGTT
  GCAGGTCTCCGGGACTCCAGTGCGCCAGCTGCAGAAGGGTGCCTGTCCCTCTGGTCTCCACCAGCTGACC
  AGTCCGCGGTACAAGTTCAACTTCATCGCCGATGTGGTGGAGAAGATTGCGCCAGCTGTGGTCCACATAG
  AGCTCTTTCTGAGACACCCCCTGTTTGGCCGGAATGTGCCGCTGTCCAGTGGCTCGGGCTTCATCATGTC
  AGAAGCCGGTTTGATCGTCACCAACGCCCACGTGGTCTCCAGCTCCAGCACTGCCTCCGGCCGGCAGCAG
  CTGAAGGTGCAGCTGCAGAATGGGGATGCCTATGAGGCCACCATCCAGGACATCGACAAGAAGTCGGACA
  TTGCCACGATTGTAATCCACCCCAAGAAAAAGCTCCCTGTGTTGCTGCTGGGTCACTCAGCAGACCTGCG
  GCCTGGCGAGTTCGTGGTGGCCATCGGCAGCCCCTTTGCCCTGCAGAACACCGTGACAACGGGCATTGTC
  AGCACTGCCCAGCGGGATGGCAAGGAGCTGGGTCTCCGGGACTCAGACATGGACTATATCCAGACCGATG
  CCATCATCAATTACGGGAACTCAGGAGGACCCCTGGTGAACCTGGATGGCGAGGTCATCGGCATCAACAC
  GCTCAAGGTTGCAGCTGGCATCTCCTTTGCCATCCCCTCAGATCGCATCACACGCTTCCTCTCTGAGTTC
  CAAAACAAGCATGTGAAAGACTGGAAGAAGCGCTTCATTGGCATCCGGATGCGGACCATCACGCCAAGTT
  TGGTGGAGGAACTGAAGGCCGCCAACCCAGACTTTCCAGCGGTCAGCAGTGGAATATATGTTCAAGAGGT
  GGTTCCCAATTCACCTTCTCAGAGAGGAGGCATCCAAGATGGCGACATCATCGTCAAAGTCAATGGCCGC
  CCCCTGGCGGATTCCAGCGAGCTGCAGGAGGCAGTCCTGAACGAGTCTTCACTCCTGCTGGAGGTGCGGC
  GAGGCAATGATGATCTCCTCTTCAGCATCATCCCTGAGGTGGTCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226669 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQARALLPATLAILATLAVLALAREPPAAPCPARCDVSRCPSPRCPGGYVPDLCNCCLVCAASEGEPCGR
  PLDSPCGDSLECVRGVCRCRWTHTVCGTDGHTYADVCALQAASRRALQVSGTPVRQLQKGACPSGLHQLT
  SPRYKFNFIADVVEKIAPAVVHIELFLRHPLFGRNVPLSSGSGFIMSEAGLIVTNAHVVSSSSTASGRQQ
  LKVQLQNGDAYEATIQDIDKKSDIATIVIHPKKKLPVLLLGHSADLRPGEFVVAIGSPFALQNTVTTGIV
  STAQRDGKELGLRDSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVAAGISFAIPSDRITRFLSEF
  QNKHVKDWKKRFIGIRMRTITPSLVEELKAANPDFPAVSSGIYVQEVVPNSPSQRGGIQDGDIIVKVNGR
  PLADSSELQEAVLNESSLLLEVRRGNDDLLFSIIPEVVM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030127
• ORF Size: 1377 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030127.3, NP_084403.2
• RefSeq Size: 2754 bp
• RefSeq ORF: 1380 bp
• Locus ID: 78558
• UniProt ID: Q9D236
• Cytogenetics: 5 B3
• MW: 49.1 kDa
• Gene Summary: Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (PubMed:15206957). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (By similarity).[UniProtKB/Swiss-Prot Function]