Men1 (NM_008583) Mouse Tagged ORF Clone

CAT#: MR226666

Men1 (Myc-DDK-tagged) - Mouse multiple endocrine neoplasia 1 (Men1), transcript variant 2

Product Images

Circular map for MR226666

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Men1
• Synonyms: AW045611
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226666 representing NM_008583
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGAAGGCCGCCCAGAAGACGCTGTTCCCTCTGCGCTCTATCGACGACGTGGTGCGCCTGTTTG
  CTGCAGAGCTGGGCCGAGAGGAGCCTGACCTGGTGCTCCTGTCCTTGGTCCTGGGCTTCGTGGAGCATTT
  CCTGGCTGTCAACCGTGTCATCCCCACCAACGTGCCGGAGCTCACCTTCCAGCCCAGCCCCGCACCCGAC
  CCTCCTGGTGGCCTCACCTACTTCCCGGTGGCCGACCTATCCATCATTGCTGCCCTCTATGCCCGATTCA
  CCGCTCAGATCCGCGGCGCTGTGGACCTCTCCCTCTATCCTCGAGAGGGAGGTGTTTCTAGTCGCGAACT
  GGTAAAAAAGGTCTCGGATGTCATATGGAACAGCCTCAGCCGCTCCTACTTCAAGGACCGGGCCCACATC
  CAGTCCCTCTTCAGCTTCATCACAGGCACCAAACTGGACAGCTCGGGCGTGGCCTTTGCAGTGGTAGGGG
  CCTGCCAGGCTCTGGGTCTCAGAGATGTCCATCTGGCCCTGTCTGAAGATCATGCTTGGGTGGTGTTTGG
  GCCCAACGGGGAGCAGACAGCTGAGGTGACGTGGCACGGCAAAGGCAACGAGGACCGCAGAGGCCAGACA
  GTCAATGCCGGTGTGGCTGAGCGGAGCTGGCTGTACCTGAAGGGGTCGTACATGCGCTGCGACCGTAAGA
  TGGAGGTGGCGTTCATGGTGTGTGCCATCAACCCTTCCATCGATCTTCACACTGACTCTTTGGAACTGTT
  GCAACTACAGCAGAAGCTGCTCTGGCTGCTGTATGACCTCGGACATCTGGAAAGATACCCCATGGCGCTA
  GGGAACTTGGCAGACCTGGAGGAGCTGGAGCCTACCCCGGGCCGGCCAGACCCACTCACCCTTTATCACA
  AGGGAATTGCCTCAGCTAAGACCTACTACCAGGATGAACACATCTACCCCTACATGTACCTGGCTGGCTA
  CCACTGTCGCAACCGAAATGTGCGCGAAGCCCTGCAGGCCTGGGCCGACACTGCCACTGTTATCCAAGAC
  TACAACTACTGCCGGGAGGATGAGGAGATCTACAAGGAATTCTTTGAAGTGGCCAATGACGTCATCCCCA
  ACCTGCTGAAGGAGGCGGCCAGCCTGCTAGAAACAGGCGAAGAGCGGACTGGGGAGCAAGCCCAGGGCAC
  CCAGGGCCAAGGTTCTGCTCTCCAGGACCCAGAATGCTTTGCCCACCTGCTGCGATTTTATGATGGCATC
  TGCAAATGGGAGGAGGGTAGCCCCACACCAGTACTGCACGTAGGCTGGGCAACTTTCCTTGTGCAGTCCC
  TAGGCCGCTTCGAGGGACAGGTGCGGCAGAAGGTGCACATAGTTAGCCGGGAAGCAGAGGCGGCCGAGGC
  TGAAGAACCATGGGGGGATGAAGCCCGAGAAGGCCGTCGGCGTGGTCCCCGAAGAGAGTCCAAGCCTGAG
  GAGCCACCACCACCCAAGAAGCCTGCATTGGACAAGGGCCCGGGCTCAGGACAAAGTGCAGGGTCGGGAC
  CACCTAGGAAAACGTCAGGGACTGTCCCAGGTACTACCCGCGGTGGCCAGGAAGTCGGCAATGCTGCTCA
  GGCTCCAGCACCTGCAGCATCGCCACCCCCAGAGGGCCCAGTGCTCACTTTCCAGAGTGAGAAGATGAAA
  GGCATGAAGGAGCTACTGGTGGCCACCAAGATCAACTCGAGCGCCATCAAGCTGCAACTCACGGCACAGT
  CGCAAGTGCAGATGAAGAAACAGAAAGTGTCCACACCCAGCGACTACACACTCTCTTTCCTAAAGCGGCA
  GCGCAAGGGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226666 representing NM_008583
  Red=Cloning site Green=Tags(s)
  
  MGLKAAQKTLFPLRSIDDVVRLFAAELGREEPDLVLLSLVLGFVEHFLAVNRVIPTNVPELTFQPSPAPD
  PPGGLTYFPVADLSIIAALYARFTAQIRGAVDLSLYPREGGVSSRELVKKVSDVIWNSLSRSYFKDRAHI
  QSLFSFITGTKLDSSGVAFAVVGACQALGLRDVHLALSEDHAWVVFGPNGEQTAEVTWHGKGNEDRRGQT
  VNAGVAERSWLYLKGSYMRCDRKMEVAFMVCAINPSIDLHTDSLELLQLQQKLLWLLYDLGHLERYPMAL
  GNLADLEELEPTPGRPDPLTLYHKGIASAKTYYQDEHIYPYMYLAGYHCRNRNVREALQAWADTATVIQD
  YNYCREDEEIYKEFFEVANDVIPNLLKEAASLLETGEERTGEQAQGTQGQGSALQDPECFAHLLRFYDGI
  CKWEEGSPTPVLHVGWATFLVQSLGRFEGQVRQKVHIVSREAEAAEAEEPWGDEAREGRRRGPRRESKPE
  EPPPPKKPALDKGPGSGQSAGSGPPRKTSGTVPGTTRGGQEVGNAAQAPAPAASPPPEGPVLTFQSEKMK
  GMKELLVATKINSSAIKLQLTAQSQVQMKKQKVSTPSDYTLSFLKRQRKGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008583
• ORF Size: 1833 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008583.2, NP_032609.1
• RefSeq Size: 2980 bp
• RefSeq ORF: 1836 bp
• Locus ID: 17283
• UniProt ID: O88559
• Cytogenetics: 19 A
• MW: 68 kDa
• Gene Summary: Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression (By similarity). May be involved in normal hematopoiesis through the activation of HOXA9 expression. May be involved in DNA repair.[UniProtKB/Swiss-Prot Function]