Gli3 (NM_008130) Mouse Tagged ORF Clone

CAT#: MR226591

Gli3 (Myc-DDK-tagged) - Mouse GLI-Kruppel family member GLI3 (Gli3)

Product Images

Circular map for MR226591

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gli3
• Synonyms: add; AI854843; AU023367; Bph; GLI3-190; GLI3FL; Pdn; Xt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226591 representing NM_008130
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCCAGGCCCACAGCTCTACGGCGACTGAGAGGAAGAAAGCTGAAAATTCCATTGGGAAATGTC
  CCACGAGAACAGATGTCAGCGAGAAGGCCGTGGCCTCTAGTACCACTTCCAATGAGGATGAAAGTCCTGG
  ACAGATCTATCACCGAGAGAGAAGAAACGCAATCACTATGCAGCCTCAGAGTGTGCAGGGTCTCAACAAA
  ATCAGTGAGGAGCCCTCGACGTCTAGTGATGAGAGGGCCTCGCTGATCAAGAAAGAGATCCATGGCTCTC
  TACCACATCTGGCGGAGCCCTCTCTCCCTTACCGTGGGACTGTGTTTGCCATGGATCCCCGGAATGGCTA
  CATGGAGCCTCACTACCACCCTCCTCATCTTTTCCCTGCCTTCCATCCTCCTGTACCAATTGATGCCAGA
  CATCATGAGGGCCGTTACCATTATGATCCATCTCCTATTCCTCCATTACATGTGCCTTCTGCCTTATCTA
  GTAGCCCGACGTATCCAGACTTGCCCTTCATTAGGATCTCCCCACACCGTAATCCCACTGCAGCCTCAGA
  GTCCCCCTTCAGCCCCCCACACCCCTACATCAACCCATATATGGACTACATCCGCTCATTGCACAGCAGC
  CCATCCCTCTCCATGATCTCTGCTGCCCGAGGGCTGAGCCCTACAGATGCTCCCCATGCTGGAGTCAGCC
  CTGCGGAATACTATCACCAGATGGCTCTGCTGACAGGCCAGCGCAGCCCTTATGCAGACATCCTTCCCTC
  AGCTGCCACTGCTGGTGCAGGGGCCATCCATATGGAGTACCTTCATGCCATGGACAGCACCAGATTTCCC
  AGCCCTAGGCTGTCAGCTAGGCCCAGCCGAAAGCGCACACTGTCAATATCGCCACTGTCAGATCATAGCT
  TCGACCTTCAGACCATGATAAGAACATCCCCTAACTCCTTGGTTACAATCCTCAATAATTCCCGTAGCAG
  CTCTTCAGCAAGTGGTTCCTATGGGCACTTATCGGCAAGTGCAATCAGCCCTGCATTGAGCTTCACCTAC
  CCCTCCGCTCCTGTGTCTCTTCACATGCATCAACAGATCCTAAGCCGACAGCAAAGCTTAGGCTCCGCAT
  TCGGACACAGCCCTCCTCTCATCCACCCTGCTCCAACATTTCCAACACAGAGACCTATCCCTGGGATTCC
  GACAGTTCTGAACCCCGTCCAGGTCAGCTCTGGCCCTTCTGAGTCCTCACAGAGCAAGCCCACAAGCGAG
  TCTGCAGTGAGCAGCACTGGTGACCCTATGCATAATAAGCGGTCCAAGATCAAGCCTGATGAAGACCTCC
  CCAGCCCAGGGTCACGGGGCCAGCAGGAACAGCCGGAAGGAACAACCCTAGTCAAGGAGGAAGCGGACAA
  AGATGAAAGCAAGCAGGAGCCTGAAGTCATCTACGAGACAAACTGCCACTGGGAAGGCTGCACCAGAGAG
  TTCGACACCCAAGATCAGCTTGTGCATCATATCAATAATGACCACATTCATGGAGAAAAGAAGGAGTTCG
  TGTGCCGCTGGCTTGATTGTTCACGAGAGCAGAAACCGTTCAAAGCCCAGTACATGTTGGTAGTGCATAT
  GAGAAGACACACTGGGGAGAAGCCTCACAAATGTACATTTGAAGGTTGCACAAAAGCCTACTCAAGACTC
  GAAAACCTGAAAACCCACTTGAGATCTCACACTGGAGAGAAGCCATACGTCTGTGAGCATGAGGGCTGCA
  ACAAGGCTTTCTCTAATGCTTCAGATCGGGCCAAGCACCAAAACAGAACACATTCCAATGAGAAACCGTA
  TGTATGCAAAATCCCTGGCTGCACCAAGCGTTACACAGACCCCAGCTCTCTCCGGAAACACGTGAAGACT
  GTGCATGGCCCTGAGGCTCATGTTACCAAGAAGCAGCGTGGGGACATGCACCCTCGGCCTCCACCCCCGA
  GAGATTCCGGCAGCCATTCACAGTCCAGGTCCCCTGGCCGGCCAACTCAGGGAGCATTTGGTGAGCAGAA
  GGAGCTGAGCAACACTACCTCAAAGCGGGAAGAGTGCCTCCAGGTGAAGACTGTCAAGGCTGAGAAGCCC
  ATGACATCTCAGCCAAGCCCTGGTGGTCAGTCTTCATGCAGCAGCCAACAGTCCCCCATCAGCAACTATT
  CCAACAGTGGGCTCGAGCTTCCTCTGACTGATGGAGGTAGTGTAGCAGACCTCAGTGCCATCGATGAAAC
  CCCAATCATGGACTCGACCATTTCCACGGCAACCACAGCCCTTGCTTTGCAAGCCAGGAGAAACCCGGCA
  GGGACCAAATGGATGGAGCACATCAAACTTGAAAGGCTAAAACAAGTGAATGGAATGTTTCCAAGACTGA
  ACCCTATTCTACCCTCCAAAGCCCCTGCGGTATCTCCTCTCATAGGAAATGGCACACAGTCCAATAACAA
  CTATAGCTCGGGTGGGCCCGGGACCCTCCTCCCGAGCAGAAGTGACCTGTCAGGTGTAGACTTCACAGTG
  CTGAACACACTCAACAGGAGAGACAGCAATACCAGCACTATCAGCTCTGCCTACCTGAGCAGCCGCAGAT
  CCTCGGGCATCTCCCCCTGCTTTTCCAGCCGCAGGTCCAGCGAGGCGTCCCAGGCTGAAGGGCGACCCCA
  GAATGTGAGTGTGGCTGACTCCTACGATCCCATCTCCACAGATGCTTCAAGGAGGTCCAGCGAGGCCAGC
  CAGGGTGACGGGCTGCCCAGTCTGCTCAGCCTCACACCCGTGCAGCAGTACCGCCTTAAGGCCAAGTATG
  CAGCTGCCACAGGTGGCCCACCACCCACACCTCTGCCCCACATGGAGAGGCTGAGCCTGAAGACCAAGAT
  GGCCTTGCTAGGGGAAGGGAGGGACTCTGGGGTGACCCTGCCTCCAGTCCATCCTCCTCGAAGATGCAGT
  GATGGGGGAGGTCACACATACAGAGGGCGTCACCTGATGCCTCATGATGCACTAGCGAACAGTGTGAGGA
  GAGCCAGCGACCCTGTGAGGACAGTCTCGGAGAACATGTCACTTGCCAGGGTTCAACGCTTCAGCAGCCT
  CAATAGCTTTAATCCTCCAAATTTGCCTCCATCGGTGGAAAAGCGTAGCTTAGTGCTGCAAAATTATACC
  CGGCAGGAGAGCAGCCAACCCCGGTACTTCCAGGCATCCCCTTGCCCTCCCAGCATCACAGAGAATGTTG
  CCCTGGAGGCTCTGACCATGGATGCTGATGCTAACTTGAATGATGAAGACCTCCTGCCAGATGATGTGGT
  ACAGTATTTAAATTCCCAGAACCAAACAGGGTATGGGCAGCAGTTGCAGAGTGGCATCTCTGAAGACAGC
  AAAGTAGCCCATGAGCCAGAGGACTTGGACTTAGCAGGGCTGCCAGACAGTCATGTTGGCCAGGAGTACC
  CAGCTCTGGAGCAGCCCTGCTCTGAGGGCAGTAAAACTGATTTGCCCATCCAGTGGAACGAGGTCAGTTC
  TGGAACTTCTGATCTGTCATCCTCCAAGCTGAAGTGTGGTCAGCAGCGCCCTAGCGCACAGCAGCCTCGG
  GGTTTTGGGCTATACAACAATATGGTGGTACACCCACATAATCTGTGGAAGGTTGGCACTGGCCCAGCTG
  GGGGATATCAGACCCTCGGGGAGAATAGCAGTACCTACAATGGCCCAGAGCACTTTGCAATCCACAGTGG
  GGATGGACTTGGCACCAATGGAAATACTTTCCATGAACAGCCCTTTAAGACCCAGCAGTATGGGAGCCAG
  CTCAACAGGCAGCCACTGACTTCCAGTGCTCTAGATCATGCCTGTGGTACAGGGATTCAAGGGTCCAAGC
  TAAAAGGCAACAGCTTGCAAGAGAATGGGGGTTTGCTAGATTTCAGCCTGTCCGTGGCACCAAATGAGTT
  AGCTGGCAACACAGTGAATGGCATGCAAACCCAAGATCAAATGGGACAGGGATACATTGCCCATCAGCTA
  CTCAGTGGCAGCATGCAACACCAGGGGCCCAGCCGCCCTGGTCAACAGGTACTAGGGCAGGTTGGTGCTA
  CCTCACATATCAACATCTATCAAGGGACAGAGAGCTGCCTACCAGGGACTCAGGACAACAGCAGCCAGCC
  ATCAAGCATGGCAGCTATCAGGGGCTACCAGCCCTGTGCCAGCTATGGGGGTAACAGGCGTCAGGCAATG
  CCAAGGGGCAACCTCACTCTGCAACAAGGACAGCTCAGTGACATGAGTCAGAGCAGCAGGGTGAACAGCA
  TCAAAATGGAGGCACAAGGTCAGTCCCAGCAGCTCTGCTCTACCGTGCAGAATTATTCCGGTCAGTTCTA
  TGACCAAACCATGGGCTTCAGTCAGCAAGACAGGAAAGCTGGCTCGTTCTCCCTCTCAGATGCCAACTGC
  CTGCTCCAAGGGAATGGCACTGAAAACTCTGAGTTACTCTCCCCAGGTGCTAACCAGGTAACAAGCACAG
  TTGACAGCTTTGAGAGTCATGACCTAGAAGGTGTGCAGATTGATTTTGATGCCATCATAGATGATGGGGA
  CCATACCAGCCTAATGTCAGGGGCCTTGAGCCCAAGTATTATTCAGAACCTTTCCCACAGCTCCTCCCGT
  CTCACCACTCCGCGGGCATCCCTCCCATTCCCATCCCTATCCATGGGCACCACCAACATGGCTATCGGGG
  ATATGAGTTCTTTGCTGACCTCCCTTGCAGAAGAAAGCAAGTTCCTTGCAGTTATGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226591 representing NM_008130
  Red=Cloning site Green=Tags(s)
  
  MEAQAHSSTATERKKAENSIGKCPTRTDVSEKAVASSTTSNEDESPGQIYHRERRNAITMQPQSVQGLNK
  ISEEPSTSSDERASLIKKEIHGSLPHLAEPSLPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDAR
  HHEGRYHYDPSPIPPLHVPSALSSSPTYPDLPFIRISPHRNPTAASESPFSPPHPYINPYMDYIRSLHSS
  PSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADILPSAATAGAGAIHMEYLHAMDSTRFP
  SPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTY
  PSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQSKPTSE
  SAVSSTGDPMHNKRSKIKPDEDLPSPGSRGQQEQPEGTTLVKEEADKDESKQEPEVIYETNCHWEGCTRE
  FDTQDQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRL
  ENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKT
  VHGPEAHVTKKQRGDMHPRPPPPRDSGSHSQSRSPGRPTQGAFGEQKELSNTTSKREECLQVKTVKAEKP
  MTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSVADLSAIDETPIMDSTISTATTALALQARRNPA
  GTKWMEHIKLERLKQVNGMFPRLNPILPSKAPAVSPLIGNGTQSNNNYSSGGPGTLLPSRSDLSGVDFTV
  LNTLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEAS
  QGDGLPSLLSLTPVQQYRLKAKYAAATGGPPPTPLPHMERLSLKTKMALLGEGRDSGVTLPPVHPPRRCS
  DGGGHTYRGRHLMPHDALANSVRRASDPVRTVSENMSLARVQRFSSLNSFNPPNLPPSVEKRSLVLQNYT
  RQESSQPRYFQASPCPPSITENVALEALTMDADANLNDEDLLPDDVVQYLNSQNQTGYGQQLQSGISEDS
  KVAHEPEDLDLAGLPDSHVGQEYPALEQPCSEGSKTDLPIQWNEVSSGTSDLSSSKLKCGQQRPSAQQPR
  GFGLYNNMVVHPHNLWKVGTGPAGGYQTLGENSSTYNGPEHFAIHSGDGLGTNGNTFHEQPFKTQQYGSQ
  LNRQPLTSSALDHACGTGIQGSKLKGNSLQENGGLLDFSLSVAPNELAGNTVNGMQTQDQMGQGYIAHQL
  LSGSMQHQGPSRPGQQVLGQVGATSHINIYQGTESCLPGTQDNSSQPSSMAAIRGYQPCASYGGNRRQAM
  PRGNLTLQQGQLSDMSQSSRVNSIKMEAQGQSQQLCSTVQNYSGQFYDQTMGFSQQDRKAGSFSLSDANC
  LLQGNGTENSELLSPGANQVTSTVDSFESHDLEGVQIDFDAIIDDGDHTSLMSGALSPSIIQNLSHSSSR
  LTTPRASLPFPSLSMGTTNMAIGDMSSLLTSLAEESKFLAVMQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008130
• ORF Size: 4749 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008130.3
• RefSeq Size: 8428 bp
• RefSeq ORF: 4752 bp
• Locus ID: 14634
• UniProt ID: Q61602
• Cytogenetics: 13 5.43 cM
• MW: 172.1 kDa
• Gene Summary: Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.[UniProtKB/Swiss-Prot Function]