Usp4 (NM_011678) Mouse Tagged ORF Clone

CAT#: MR226489

Usp4 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (Usp4)

Product Images

Circular map for MR226489

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Usp4
• Synonyms: F730026I20Rik; mKIAA4155; Unp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226489 representing NM_011678
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAGGCCGGGGCAGCCGTGAGCGACCGGATGTGGAGACCCAGAAGACGGAGCTCGGAGCCTTGA
  TGGGGACCACGCTCCAACGTGGGGCGCAGTGGTATCTTATTGACAGCCGGTGGTTCAAGCAGTGGAAGAA
  GTATGTGGGCTTTGACAGCTGGGACATGTACAATGTCGGGGAGCATAACCTGTTTCCTGGACCTATTGAC
  AACTCCGGACTCTTCTCAGATCCTGAGAGTCAGACCTTGAAGGAGCACTTAATCGATGAGCTGGACTATG
  TGCTGGTCCCAGCCGAAGCCTGGAATAAATTGCTGAATTGGTATGGCTGTGTGGAGGGCCAGCAGCCTAT
  TGTCAGAAAAGTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCAAAGTGGAAGTGTATTTGCTGGAGCTG
  AAGCTCTGTGAGAACAGTGACCCCACCAATGTGCTAAGTTGCCATTTTAGCAAAGCAGACACCATTGCAA
  CTATTGAGAAGGAGATGAGGAAGCTCTTCAACATCCCTGCAGAACGTGAAACACGGCTTTGGAACAAATA
  CATGAGCAACACCTATGAGCAGTTGAGCAAGCTAGACAACACTATCCAGGATGCTGGGCTGTACCAGGGT
  CAGGTGCTAGTAATTGAGCCCCAAAATGAAGATGGCACATGGCCCCGGCAGAGCCTGCAGTCAAAATCAA
  GCACTGCACCTAGCAGAAATTTCACTACCTCTTCAAAACCATCCGCAAGTCCCTATTGCTCAGTGTCTGC
  CTCTCTCATTGCAAATGGTGATAGCACTAACAGCTCTGGGATGCACAGCTCCGGTGTCAGCAGGGGTGGA
  TCTGGCTTCTCTGCTTCGTATAATTGCCAGGAGCCCCCATCACCTCATATACAGCCTGGCCTCTGTGGAC
  TTGGAAACCTGGGGAACACTTGCTTCATGAACTCTGCTCTGCAGTGCCTGAGCAACACTGCCCCACTGAC
  TGAGTACTTTCTCAAAGATGAGTATGAGGCCGAGATCAACCGAGACAACCCTCTGGGGATGAAAGGGGAG
  ATTGCAGAGGCCTATGCAGAGCTCATCAAGCAGATGTGGTCTGGAAGGGACACTCACGTGGCACCACGGA
  TGTTCAAGACGCAAGTGGGACGTTTTGCCCCTCAGTTTTCTGGCTACCAACAGCAAGACTCCCAGGAGCT
  GCTAGCCTTTATTCTAGACGGACTGCACGAGGACCTGAACCGCGTAAAGAAGAAGCCTTACCTGGAGCCC
  AAGGACGCCAATGGGCGACCAGATGCGGTGGTAGCAAAGGAAGCCTGGGAAAACCACAGGCTGAGGAATG
  ATTCTGTGATTGTGGATACTTTCCATGGCCTTTTCAAATCGACTTTGGTTTGCCCAGAATGTGCTAAAGT
  TTCTGTGACCTTTGACCCATTTTGCTATCTAACTCTCCCACTGCCTTTGAAGAAGGATCGGATTATGGAG
  GTCTTCCTGGTTCCTGCTGACCCTCAGTGCAGACCTATCCAGTACCGTGTGACTGTGCCATTGATGGGGG
  CCATTTCTGACCTGTGTGAAGCACTCTCCAAGCTGTCTGGCATTGCTGCAGAAAATATGGTGGTCACTGA
  TGTATATAATCACCGCTTCCACAAAATTTTCCAAATGGATGAAGGTTTAAGCCACATCACGCCTCGAGAT
  GACATTTTTGTGTATGAGGTCTGCAACACGTCCATGGATGGCTCAGAGTGTATCACTCTTCCAGTCTACT
  TCAGAGAGAAGAAGTCCAGGCCGTCGAGTGCTTCCTCGGGGGCTGTGCTCTATGGACAGCCCCTTCTTGT
  CTCTGTCCCTAAGCATAAGCTAACCCTAGAGTCTTTGTACCAGGCTGTTTGTGATCGTATCAGCCGCTAC
  ATAAAACAGCCTTTGCCTGATGAGTTTCTCAGCTCACCCTTAGAGCCTGGGGCCTGCAATGGCTCTAGGA
  GTAGCTATGAAGGAGATGAAGAGGAAGAAATGGATCATCAAGAGGAAGGAAAAGAGCAGCTTTCCGAAGT
  GGAAGGCAGTGGTGAGGACGATCAGGGAGATGACCATAGCGAGAGCGCCCAAAAGGTGAAAGGCCAGCCG
  AGGCACAAGAGGCTCTTTACCTTCAGCCTCGTGAACTCCTGTGGAACTGCTGATATCAATTCACTGGCAA
  CTGACGGGAAACTCCTCAAACTCAACTCTCGATCCACACTGGCCATTGACTGGGACAGTGAGACCCGAAG
  CCTTTACTTTGATGAGCAAGAATCTGAGGCCTGTGAGAAGCACCTGAGCATGTCACAGCCGCAGAAGAAG
  AAGAAGGCGGCAGTAGCCCTGCGAGAGTGCATCGAGCTCTTCACCACCATGGAGACCCTTGGGGAGCATG
  ACCCCTGGTACTGTCCCACCTGTAAGAAGCACCAGCAGGCAACAAAGAAGTTTGACTTGTGGTCTTTGCC
  CAAGATCCTGGTGGTTCACCTCAAGCGTTTCTCCTATAACAGATACTGGCGGGATAAACTTGACACCGTG
  GTGGAGTTCCCAGTCAGAGCTCTGAACATGTCCGAGTTTGTCTGTGACCGGTCAGCAAGGCCTTATGTTT
  ATGACCTAATTGCTGTGTCCAATCACTATGGAGCCATGGGGGTTGGTCACTACACTGCATATGCGAAGAA
  CAGACTGAACGGGAAATGGTATTACTTTGATGATAGCAGTGTGTCCCTGGCCTCTGAGGACCAGATAGTG
  ACGAAAGCCGCCTACGTGTTGTTCTATCAGCGTCGGGATGACGAATGCTCCAGCACATCTTCGCTCGGCA
  GTTTCCCCGGTTCTGATGGAGGGGTGAAGCTGAGCAGCTCACACCAGGGCATGGGGGATGAGGAGGCTTA
  CAACATGGACACCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226489 representing NM_011678
  Red=Cloning site Green=Tags(s)
  
  MAEGRGSRERPDVETQKTELGALMGTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPID
  NSGLFSDPESQTLKEHLIDELDYVLVPAEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLEL
  KLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTIQDAGLYQG
  QVLVIEPQNEDGTWPRQSLQSKSSTAPSRNFTTSSKPSASPYCSVSASLIANGDSTNSSGMHSSGVSRGG
  SGFSASYNCQEPPSPHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLTEYFLKDEYEAEINRDNPLGMKGE
  IAEAYAELIKQMWSGRDTHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFILDGLHEDLNRVKKKPYLEP
  KDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRIME
  VFLVPADPQCRPIQYRVTVPLMGAISDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLSHITPRD
  DIFVYEVCNTSMDGSECITLPVYFREKKSRPSSASSGAVLYGQPLLVSVPKHKLTLESLYQAVCDRISRY
  IKQPLPDEFLSSPLEPGACNGSRSSYEGDEEEEMDHQEEGKEQLSEVEGSGEDDQGDDHSESAQKVKGQP
  RHKRLFTFSLVNSCGTADINSLATDGKLLKLNSRSTLAIDWDSETRSLYFDEQESEACEKHLSMSQPQKK
  KKAAVALRECIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTV
  VEFPVRALNMSEFVCDRSARPYVYDLIAVSNHYGAMGVGHYTAYAKNRLNGKWYYFDDSSVSLASEDQIV
  TKAAYVLFYQRRDDECSSTSSLGSFPGSDGGVKLSSSHQGMGDEEAYNMDTN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011678
• ORF Size: 2886 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011678.2, NP_035808.2
• RefSeq Size: 3686 bp
• RefSeq ORF: 2889 bp
• Locus ID: 22258
• UniProt ID: P35123
• Cytogenetics: 9 59.25 cM
• MW: 108.8 kDa
• Gene Summary: Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins. Deubiquitinates PDPK1. Deubiquitinates TRIM21. Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER.[UniProtKB/Swiss-Prot Function]