Usp17la (NM_007887) Mouse Tagged ORF Clone

CAT#: MR226473

Usp17la (Myc-DDK-tagged) - Mouse deubiquitinating enzyme 1 (Dub1)

Product Images

Circular map for MR226473

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Usp17la
• Synonyms: Dub1; USP17-A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226473 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGTTGCTCTTTCCTTCCCAGAAGCAGATCCAGCCCTATCATCTCCTGATGCCCCAGAGCTGCATC
  AGGATGAAGCTCAGGTGGTGGAGGAGCTAACTGTCAATGGAAAGCACAGTCTGAGTTGGGAGAGTCCCCA
  AGGACCAGGATGCGGGCTCCAGAACACAGGCAACAGCTGCTACCTGAATGCAGCCCTGCAGTGCTTGACA
  CACACACCACCTCTAGCTGACTACATGCTGTCCCAGGAGCACAGTCAAACCTGTTGTTCCCCAGAAGGCT
  GTAAGATGTGTGCTATGGAAGCCCATGTGACCCAGAGTCTCCTGCACTCTCACTCGGGGGATGTCATGAA
  GCCCTCCCATATTTTGACCTCTGCCTTCCACAAGCACCAGCAGGAAGATGCCCACGAGTTTCTCATGTTC
  ACCTTGGAAACAATGCATGAATCCTGCCTTCAAGTGCACAGACAATCAAAACCCACCTCTGAGGACAGCT
  CACCCATTCATGACATATTTGGAGGCTGGTGGAGGTCTCAGATCAAGTGTCTCCTTTGCCAGGGTACCTC
  AGATACCTATGATTGCTTCCTGGACATCCCCCTGGATATCAGCTCAGCTCAGAGTGTAAAGCAAGCCTTG
  TGGGATACAGAGAAGTCAGAAGAGCTATGTGGAGATAATGCCTACTACTGTGGTAAGTGTAGAGAGAAGA
  TGCCAGCTTCTAAGACCCTGCATGTTCATATTGCTCCAAAGGTACTCATGGTAGTGTTAAATCGTTTCTC
  AGCCTTCACGGGTAACAAGTTAGACAGAAAAGTAAGCTACCCGGAGTTCCTTGACCTGAAGCCATACCTG
  TCTGAGCCTACTGGAGGACCTTTGCCTTATGCCCTCTATGCCGTCCTGGTCCATGATGGTGCGACTTCTC
  ACAGTGGACATTACTTCTGTTGTGTCAAAGCTGGTCATGGGAAGTGGTACAAGATGGATGATACTAAAGT
  CACCAGGTGTGATGTGACTTCTGTCCTGAATGAGAATGCCTATGTGCTCTTCTATGTGCAGCAGGCCAAC
  CTCAAACAGGTCAGTATTGACATGCCAGAGGGAAGAATAAATGAGGTTCTTGACCCTGAATACCAGCTGA
  AGAAATCACGGAGAAAAAAGCATAAGAAGAAAAGCCCTTTCACAGAAGATTTAGGAGAGCCCTGCGAAAA
  CAGGGATAAGAGAGCAATTAAAGAAACCTCCTTAGGAAAGGGGAAAGTGCTTCAGGAAGTGAACCACAAG
  AAAGCTGGGCAGAAACACGGGAATACCAAACTCATGCCTCAGAAACAGAACCACCAGAAAGCTGGGCAGA
  ACCTCAGGAATACTGAAGTTGAACTTGATCTGCCTGCTGATGCAATTGTGATTCACCAGCCCAGATCCAC
  TGCAAACTGGGGCAGGGATTCTCCAGACAAGGAGAATCAACCCTTGCACAATGCTGACAGGCTCCTCACC
  TCTCAGGGCCCTGTGAACACTTGGCAGCTCTGTAGACAGGAAGGGAGACGAAGATCGAAGAAGGGGCAGA
  ACAAGAACAAGCAAGGGCAGAGGCTTCTGCTTGTTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226473 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVVALSFPEADPALSSPDAPELHQDEAQVVEELTVNGKHSLSWESPQGPGCGLQNTGNSCYLNAALQCLT
  HTPPLADYMLSQEHSQTCCSPEGCKMCAMEAHVTQSLLHSHSGDVMKPSHILTSAFHKHQQEDAHEFLMF
  TLETMHESCLQVHRQSKPTSEDSSPIHDIFGGWWRSQIKCLLCQGTSDTYDCFLDIPLDISSAQSVKQAL
  WDTEKSEELCGDNAYYCGKCREKMPASKTLHVHIAPKVLMVVLNRFSAFTGNKLDRKVSYPEFLDLKPYL
  SEPTGGPLPYALYAVLVHDGATSHSGHYFCCVKAGHGKWYKMDDTKVTRCDVTSVLNENAYVLFYVQQAN
  LKQVSIDMPEGRINEVLDPEYQLKKSRRKKHKKKSPFTEDLGEPCENRDKRAIKETSLGKGKVLQEVNHK
  KAGQKHGNTKLMPQKQNHQKAGQNLRNTEVELDLPADAIVIHQPRSTANWGRDSPDKENQPLHNADRLLT
  SQGPVNTWQLCRQEGRRRSKKGQNKNKQGQRLLLVC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007887
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007887.1, NM_007887.2, NP_031913.1
• RefSeq Size: 2661 bp
• RefSeq ORF: 1581 bp
• Locus ID: 13531
• UniProt ID: Q61068
• Cytogenetics: 7 55.69 cM
• MW: 59.1 kDa
• Gene Summary: Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes. Has deubiquitinating enzyme activity for DNAH5, suggesting a role in the regulation of DNAH5 degradation by the ubiquitin-proteasome pathway. Has growth-suppressing activity; induces arrest in G1 phase upon controlled expression.[UniProtKB/Swiss-Prot Function]