Srsf11 (NM_001093752) Mouse Tagged ORF Clone

CAT#: MR226399

Srsf11 (Myc-DDK-tagged) - Mouse serine/arginine-rich splicing factor 11 (Srsf11), transcript variant 2

Product Images

Circular map for MR226399

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Srsf11
• Synonyms: 0610009J05Rik; 2610019N13Rik; BF642805; Sfr; Sfrs11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226399 representing NM_001093752
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCACCGCCAGCTCCCGGGACCGCAGGACCGACGAGCCCTTCCGTTGCCCTCACCAACCTCGCCC
  TCAGGAGAAACTTCTTTCGGGAGGCAGCCCGCGCCATGAGCAGCACGGCAGTGGTCCCCAGCGCGCCAGG
  CCCGGGCCCAGGCCCCAGTGGCGGGCCAGGCGGCGGCACCGAGGTGATCCAGGTGACCAACGTGTCTCCG
  AGCGCCAGCTCAGAGCAGATGCGTACGCTCTTCGGCTTCCTGGGCAAGATCGACGAGCTGCGCCTCTTCC
  CGCCGGATGACTCACCTTTGCCAGTCTCCTCTCGTGTCTGCTTTGTTAAGTTCCATGATCCAGACTCAGC
  AGTTGTGGCACAGCATCTGACAAACACTGTGTTCGTTGACAGAGCTTTGATAGTCGTACCATATGCTGAA
  GGAGTTATTCCTGATGAGACTAAGGCTTTGTCTCTATTGGCACCAGCTAATGCAGTGGCAGGTCTTCTGC
  CTGGTGGTGGACTCCTGCCTACCCCCAACCCACTTACCCAGATTGGCGCTGTTCCCCTGGCTGCGTTGGG
  AGCTCCAGCTCTTGATCCTGCCCTTGCTGCTCTTGGGCTTCCAGGAACAAACTTGAACTCTCAGTCCCTT
  GCTGCAGACCAGCTATTGAAACTTATGAGTACTGTTGATCCCAAATTGAATCATGTAGCAGCTGGTCTTG
  TTTCACCAAGTCTGAAGTCAGACACCTCTAGTAAAGAAATAGAGGAAGCCATGAAGAGAGTGCGTGAAGC
  CCAGTCCCTGATTTCTGCCGCTATTGAGCCAGACAAGAAGGAAGAAAAGAGAAGACATTCGAGATCAAGA
  TCTCGCTCGAGGAGGAGGAGGACCCCCTCATCCTCCAGACACAGGCGGTCACGAAGCCGGTCAAGGAGGC
  GGTCACACTCCAAATCCAGGAGTAGAAGGAGATCCAAAAGTCCAAGACGGAGACGATCTCATTCCCGAGA
  GAGGGGTAGAAGGTCAAGGAGCACATCCAAAGCCAGAGACAAAAAGAAAGAAGATAAAGAAAAGAAACGT
  TCCAAAACTCCACCAAAAAGCTACAGCACAGCCAGACGCTCTCGGAGTGCAAGCAGAGAGAGAAGACGAC
  GGCGAAGTAGGAGTGGTACAAGATCCCCTAAAAAGCCCAGGTCTCCTAAAAGAAAGCTGTCTCGCTCACC
  ATCCCCAAGGAGACATAAAAAAGAGAAGAAGAAAGATAAGGACAAAGAAAGAAGCAGAGATGAAAGGGAA
  CGATCAACAAGTAAAAAGAAGAGGAGCAAAGACAAGGAGAAGGAGCGAGAAAGGAAGTCTGAGAGTGACA
  AAGACGTCAAACAGGTGACTCGGGATTATGATGAAGAGGAGCAAGGCTATGACAGTGAGAAGGAAAAGAA
  AGAGGAGAAGAGACCAACAGAAGCAGTGTCCCCTAAAACAAAGGAGTGCTCTGTGGAGAAGGGGGTGGGC
  GACCTTCGAGAGTCCAAAGTGAACGGGGATGATCACCACGAAGAAGACATGGATATGAGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226399 representing NM_001093752
  Red=Cloning site Green=Tags(s)
  
  MSSPPAPGTAGPTSPSVALTNLALRRNFFREAARAMSSTAVVPSAPGPGPGPSGGPGGGTEVIQVTNVSP
  SASSEQMRTLFGFLGKIDELRLFPPDDSPLPVSSRVCFVKFHDPDSAVVAQHLTNTVFVDRALIVVPYAE
  GVIPDETKALSLLAPANAVAGLLPGGGLLPTPNPLTQIGAVPLAALGAPALDPALAALGLPGTNLNSQSL
  AADQLLKLMSTVDPKLNHVAAGLVSPSLKSDTSSKEIEEAMKRVREAQSLISAAIEPDKKEEKRRHSRSR
  SRSRRRRTPSSSRHRRSRSRSRRRSHSKSRSRRRSKSPRRRRSHSRERGRRSRSTSKARDKKKEDKEKKR
  SKTPPKSYSTARRSRSASRERRRRRSRSGTRSPKKPRSPKRKLSRSPSPRRHKKEKKKDKDKERSRDERE
  RSTSKKKRSKDKEKERERKSESDKDVKQVTRDYDEEEQGYDSEKEKKEEKRPTEAVSPKTKECSVEKGVG
  DLRESKVNGDDHHEEDMDMSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001093752
• ORF Size: 1533 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001093752.3
• RefSeq Size: 3086 bp
• RefSeq ORF: 1536 bp
• Locus ID: 69207
• Cytogenetics: 3 H4
• MW: 57.1 kDa
• Gene Summary: The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]