Cacna1h (NM_001163691) Mouse Tagged ORF Clone

CAT#: MR226266

Cacna1h (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant 2

Product Images

Circular map for MR226266

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cacna1h
• Synonyms: alpha13.2; Cav3; Cav3.2; MNCb-1209
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226266 representing NM_001163691
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAGGGCACGCTGGCAGCGGACGAGGTCCGGGTGCCCCTGGGCGCTTCGCCTTCGGCTCCTGCAG
  CGCCGGTGAGGGCTTCCCCAGCGAGCCCTGGGGTGCCGGGGCGCGAGGAGCAGCGAGGATCCGGGTCGAG
  CGTGTTGGCTCCCGAGAGCCCAGGGACCGAGTGTGGTGCGGACCTGGGCGCCGACGAGGAACAGCCCGTC
  CCATACCCCGCTCTGGCTGCCACAGTCTTCTTCTGCCTCGGGCAAACCACGCGGCCCCGCAGCTGGTGCC
  TCCGACTGGTTTGTAACCCGTGGTTCGAGCACATTAGCATGCTGGTCATCATGCTGAACTGCGTGACACT
  GGGCATGTTCCGGCCCTGTGAGGATGTTGAGTGCCGTTCAGAACGTTGCAGCATCTTGGAGGCCTTTGAT
  GACTTCATCTTTGCCTTCTTTGCTGTGGAGATGGTTATCAAGATGGTGGCTTTGGGACTGTTCGGCCAGA
  AATGCTACCTGGGTGACACCTGGAACAGGCTGGACTTCTTCATTGTCATGGCTGGCATGATGGAATACTC
  TCTGGACGGACACAACGTGAGCCTCTCTGCCATCCGAACAGTGCGTGTGCTGCGGCCCCTCCGCGCCATC
  AACCGGGTCCCCAGCATGCGAATCCTGGTCACACTGCTGCTGGACACACTGCCCATGCTTGGGAACGTGC
  TTCTTCTCTGCTTCTTCGTCTTCTTCATCTTCGGCATCGTTGGGGTCCAGCTCTGGGCTGGCCTGCTGCG
  GAACCGATGCTTCCTGGACAGCGCCTTCGTCAGGAACAACAACCTGACCTTCTTGCGGCCATACTACCAG
  ACAGAGGAGGGCGAGGAGAACCCATTCATCTGCTCCTCACGCCGCGACAACGGCATGCAGAAATGCTCAC
  ACATCCCCAGCCGCCGTGAGCTTCGCGTGCAGTGCACGCTCGGCTGGGAGGCCTACGGGCAGCCACAGGC
  TGAGGATGGGGGTGCCGGCCGAAATGCCTGCATCAACTGGAACCAGTACTACAACGTGTGCCGCTCGGGG
  GAATTCAACCCTCACAATGGTGCCATCAACTTTGACAACATTGGCTATGCTTGGATCGCCATCTTCCAGG
  TCATCACACTGGAGGGCTGGGTGGACATCATGTACTACGTCATGGATGCCCACTCGTTCTACAACTTCAT
  CTACTTCATCCTCCTCATCATTGTGGGCTCCTTCTTCATGATCAACCTGTGCCTGGTGGTGATAGCCACA
  CAGTTCTCAGAGACAAAGCAGAGGGAAAACCAGCTGATGCGTGAACAGCGGGCCCGCTATCTGTCCAACG
  ACAGCACTCTGGCCAGCTTCTCGGAGCCTGGCAGCTGCTACGAGGAGCTCCTCAAGTATGTGGGCCACAT
  CTTCCGAAAGGTTAAACGCCGGAGCCTGCGCCTTTACGCCCGCTGGCAGAGCCGTTGGCGTAAGAAGGTA
  GATCCCAGCAGCACTCTGCATGGCCAAGGCCCCAGGCGGCGGCCACGGCGCGCAGGCAGGCGCACAGCTT
  CTGTGCACCATCTGGTCTACCACCACCACCACCACCATCACCACCATTACCACTTCAGCCACGGTGGCCC
  ACGCAGGCCTAGCCCAGAGCCAGGTGCTGGTGACACCAGGTTGGTCAGGGCCTGTGTGCCACCCTCGCCA
  CCATCCCCAGGCCACGGGCCACCAGACTCTGAGTCTGTGCACAGTATCTACCATGCTGACTGCCACGTGG
  AGGGGCCACAGGAAAGAGCCCGTGTGGCGCACACCATAGCCACTGCTGCCAGCCTCAAGCTGGCCTCAGG
  CTTGGGCACCATGAACTACCCCACCATCCTACCTTCAGGAGCAGTCAACAGCAAAGGCAGTACCAGCTCG
  CGACCCAAGGGGCTTCGGAGTGCTGGCACCCCAGGGGCCACAGCACACAGCCCTCTGAGCCTGGGGAGCC
  CCAGCCCCTATGAGAAGATCCAGCACGTGGTTGGAGAACAAGGACTAGGCCGAGCCTCTAGTCACCTGTC
  AGGCCTGAGTGTGCCTTGCCCCCTGCCCAGCCCCCAGGCTGGCACACTGACCTGTGAGCTGAAGAGCTGC
  CCATATTGTGCCAGCGCCCTGGAGGACCCCGAGTTTGAGTTCAGTGGCTCAGAGAGTGGGGACTCGGATG
  CCCATGGAGTCTATGAGTTTACACAGGACGTACGGCATGGGGATTGCCGAGATCCTGTGCAGCAGCCCCA
  TGAAGGGGGCACGCCGGGCCATGGCAACGAACGGTGGCGGCCACCACTGCGGACAGCCTCACAGCCAGGA
  GGGTTAGGCCGCCTCTGGGCTTCCTTTAGTAGCAAGCTGCGTCGCATTGTAGACAGCAAGTACTTCAACC
  GAGGCATCATGGCAGCCATCCTCGTCAATACTCTGAGCATGGGTGTTGAGTATCATGAGCAGCCTGATGA
  GCTGACTAACGCGCTGGAGATAAGCAACATCGTGTTCACCAGCATGTTTGCCTTGGAGATGCTACTGAAG
  TTGCTGGCCTGTGGCCCGCTGGGCTACATTCGGAACCCCTACAACATCTTTGATGGCATTGTTGTCATCA
  TAAGCGTCTGGGAGATTGTGGGGCAGGCAGATGGTGGCCTGTCAGTGCTCCGTACGTTCCGGCTGCTGCG
  GGTGCTGAAGCTGGTGCGCTTCCTGCCAGCCCTGCGGCGGCAGCTGGTGGTGCTCATGAGGACCATGGAC
  AATGTGGCCACCTTCTGCATGCTGCTAATGCTCTTCATCTTCATCTTCAGCATCCTGGGCATGCACCTGT
  TTGGCTGTAAGTTCAGCCTGAAGACAGACTCTGGAGACACCGTCCCTGACAGGAAGAACTTCGACTCTCT
  ACTGTGGGCCATCGTCACTGTGTTTCAGATCTTGACACAGGAAGACTGGAATGTGGTTCTTTACAACGGC
  ATGGCTTCCACCTCGTCCTGGGCTGCCCTTTACTTTGTGGCCCTCATGACCTTTGGGAACTATGTGCTCT
  TCAACCTGCTGGTAGCCATCCTGGTGGAAGGCTTCCAGGCAGAGGGTGATGCCACCAGATCTGACACCGA
  TGAGGATAAGACATCTACCCACCTAGAGGAAGATTTCGATAAGCTCAGAGACGTTCAAGCCACAGAGATG
  AAGATGTACTCACTGGCTGTGACCCCTAATGGGCACCTAGAGGGCCGAGGCAGTCTGCCGCCGCCTCTCA
  TCACGCACACAGCGGCTACGCCTATGCCTACCCCCAAGAGCTCCCCTCATCTGGACATGGCCCACACTCT
  TTTGGACTCACGGCGCAGCAGCAGTGGCTCTGTGGACCCCCAACTCGGGGACCAGAAGTCTCTGGCTAGC
  CTGCGCAGCTCCCCTTGTGCCCCATGGGGCCCCAACAGTGCAGGAAGCAGCCGGCGCTCCAGCTGGAACA
  GCCTGGGCCGCGCACCCAGCCTCAAACGCCGCAGCCAGTGTGGGGAGCGCGAGTCCCTGCTCTCTGGTGA
  GGGGAAGGGCAGCACGGATGATGAGGCCGAGGACAGCAGACCAAACTCAGGGACCCACCCAGGAGCTTCA
  CCCGGGCCCCGGGCCACACCCCTGCGGCGGGCTGAGTCGTTGGGCCACCGCAGCACAATGGACCTGTGCC
  CCCCACGGCCTGCCACCCTCCTGCCCACCAAGTTCCGTGACTGCAACGGGCAGATGGTGGCCCTGCCCAG
  CGAGTTCTTCCTGCGCATCGACAGCCACAAGGAGGACGCAGCAGAGTTTGATGATGACATAGAAGATAGC
  TGCTGCTTCCGCCTGCACAAAGTGCTGGAGCCCTATGCACCCCAGTGGTGCAGCAGCCGGGAGTCCTGGG
  CCCTGTATCTCTTCCCGCCGCAGAACAGGCTACGCGTCTCCTGCCAGAAAGTCATCGCACACAAGATGTT
  TGACCACGTGGTCCTTGTCTTCATCTTCCTCAACTGTATCACCATTGCCCTGGAGAGGCCAGACATCGAT
  CCGGGCAGCACTGAACGGGCCTTTCTCAGCGTCTCCAACTACATCTTCACAGCCATCTTCGTGGTGGAGA
  TGATGGTGAAGGTGGTAGCCCTGGGGCTGCTGTGGGGTGAGCATGCCTACCTGCAGAGCAGCTGGAATGT
  GCTGGACGGGCTGCTTGTCCTGGTGTCCCTGGTTGACATCATTGTGGCCGTGGCCTCTGCCGGTGGTGCC
  AAGATCCTAGGCGTCCTGCGCGTGCTGCGCCTGCTGCGGACCCTGAGGCCTCTGAGGGTCATCAGCCGAG
  CTCCAGGCCTCAAGCTGGTCGTGGAGACTCTGATATCATCGCTCAGGCCCATCGGGAACATCGTCCTCAT
  CTGCTGCGCCTTCTTCATTATCTTTGGCATCCTCGGGGTGCAGCTTTTCAAGGGCAAATTCTACTACTGC
  GAGGGTACAGATACCAGGAATATCACCACCAAGGCCGAGTGCCATGCCGCCCACTACCGCTGGGTGAGGC
  GCAAATACAACTTTGACAACCTGGGTCAGGCGCTGATGTCTCTGTTCGTGCTGTCATCCAAGGATGGCTG
  GGTAAACATCATGTACGACGGGCTGGACGCCGTGGGCATCGACCAGCAGCCTGTGCAGAACCACAACCCC
  TGGATGCTGCTCTACTTCATCTCCTTCCTGCTCATCGTCAGCTTCTTCGTGCTCAACATGTTTGTGGGCG
  TGGTGGTGGAGAACTTCCACAAGTGCCGGCAGCACCAGGAGGCGGAGGAAGCGCGGCGGCGCGAGGAGAA
  ACGGCTGCGGCGCCTGGAGAGGAGGCGCAGGAAGGCCCAGCGCCGGCCCTACTACGCAGACTATTCACAC
  ACGCGCCGCTCCATCCATTCGCTGTGCACCAGCCACTACCTGGACCTCTTCATCACCTTCATCATCTGCC
  TCAATGTCATCACCATGTCCATGGAGCACTACAACCAGCCCAAGTCTCTGGACGAGGCCCTTAAGTACTG
  CAACTACGTCTTTACCATCGTCTTCGTCTTTGAGGCCGCACTGAAGCTGGTGGCCTTTGGATTCCGGAGG
  TTCTTCAAGGACAGGTGGAACCAGCTGGACTTGGCCATCGTCCTCCTGTCCATCATGGGCATTGCGCTGG
  AGGAGATTGAGATGAATGCTGCCCTCCCCATCAACCCCACCATCATCCGCATCATGCGCGTGCTTCGCAT
  CGCCCGTGTGCTGAAGCTACTGAAGATGGCCACAGGCATGCGCGCCCTGCTGGACACTGTGGTTCAAGCT
  CTGCCTCAGGTAGGGAACCTTGGTCTTCTTTTCATGCTCCTGTTTTTTATCTATGCTGCCCTGGGAGTGG
  AGCTGTTTGGGAGGCTAGAATGCAGCGAGGATAATCCCTGTGAGGGCCTGAGCAGACACGCCACCTTCAC
  CAACTTTGGCATGGCCTTCCTCACGTTGTTCCGAGTGTCCACTGGGGACAACTGGAATGGGATCATGAAG
  GATACTCTCCGTGAATGTACCCGTGAAGACAAGCACTGCCTCAGCTACCTGCCCGCGCTCTCCCCCGTCT
  ACTTCGTCACCTTCGTGCTGGTCGCCCAGTTCGTGCTGGTCAACGTGGTGGTGGCCGTGCTCATGAAGCA
  CCTGGAGGAGAGCAACAAGGAGGCCCGGGAGGATGCGGAGATGGATGCCGAGATCGAGCTGGAGATCGCA
  CAGGGGTCCACAGCCCAGCCCCCGTCCACAGCACAGGAAAGCCAAGGTACCGAGCCAGACACCCCGAACC
  TCCTGGTCGTGCGCAAAGTATCTGTGTCCAGGATGCTCTCGCTACCCAATGACAGCTACATGTTCCGGCC
  GGTGGCTCCTGCGGCTGCCCCGCATTCCCACCCACTGCAGGAAGTGGAGATGGAGACCTACACAGGTCCC
  ATCAGCTGCGTCCTCCCCAGCCAGGGTCAGCGACCCCCTTTGTGCCCTCTCACCCCGGGATACGCCCCGC
  TCTCTGAGCCTCTCACGGATACTCTGCAGACAGGAGGCCATGCACGCAGAGTCCCTGGAAGGGCAGATAG
  A
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226266 representing NM_001163691
  Red=Cloning site Green=Tags(s)
  
  MTEGTLAADEVRVPLGASPSAPAAPVRASPASPGVPGREEQRGSGSSVLAPESPGTECGADLGADEEQPV
  PYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFD
  DFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAI
  NRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRPYYQ
  TEEGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQAEDGGAGRNACINWNQYYNVCRSG
  EFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIAT
  QFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKV
  DPSSTLHGQGPRRRPRRAGRRTASVHHLVYHHHHHHHHHYHFSHGGPRRPSPEPGAGDTRLVRACVPPSP
  PSPGHGPPDSESVHSIYHADCHVEGPQERARVAHTIATAASLKLASGLGTMNYPTILPSGAVNSKGSTSS
  RPKGLRSAGTPGATAHSPLSLGSPSPYEKIQHVVGEQGLGRASSHLSGLSVPCPLPSPQAGTLTCELKSC
  PYCASALEDPEFEFSGSESGDSDAHGVYEFTQDVRHGDCRDPVQQPHEGGTPGHGNERWRPPLRTASQPG
  GLGRLWASFSSKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLK
  LLACGPLGYIRNPYNIFDGIVVIISVWEIVGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMD
  NVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNG
  MASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTHLEEDFDKLRDVQATEM
  KMYSLAVTPNGHLEGRGSLPPPLITHTAATPMPTPKSSPHLDMAHTLLDSRRSSSGSVDPQLGDQKSLAS
  LRSSPCAPWGPNSAGSSRRSSWNSLGRAPSLKRRSQCGERESLLSGEGKGSTDDEAEDSRPNSGTHPGAS
  PGPRATPLRRAESLGHRSTMDLCPPRPATLLPTKFRDCNGQMVALPSEFFLRIDSHKEDAAEFDDDIEDS
  CCFRLHKVLEPYAPQWCSSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDID
  PGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGA
  KILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYC
  EGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNP
  WMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRKAQRRPYYADYSH
  TRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRR
  FFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQA
  LPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMK
  DTLRECTREDKHCLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAEMDAEIELEIA
  QGSTAQPPSTAQESQGTEPDTPNLLVVRKVSVSRMLSLPNDSYMFRPVAPAAAPHSHPLQEVEMETYTGP
  ISCVLPSQGQRPPLCPLTPGYAPLSEPLTDTLQTGGHARRVPGRADR
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163691
• ORF Size: 6021 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163691.1, NP_001157163.1
• RefSeq Size: 8187 bp
• RefSeq ORF: 6024 bp
• Locus ID: 58226
• Cytogenetics: 17 12.53 cM
• MW: 224.7 kDa
• Gene Summary: Voltage-dependent Ca(2+) channels mediate the entry of Ca(2+) ions into excitable cells and are involved in a variety of Ca(2+)-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. The protein encoded by this gene is an integral membrane protein that belongs to the calcium channel alpha-1 subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]