Slc12a6 (NM_133649) Mouse Tagged ORF Clone

CAT#: MR226239

Slc12a6 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 6 (Slc12a6), transcript variant 2

Product Images

Circular map for MR226239

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc12a6
• Synonyms: 9530023I19Rik; gaxp; KCC3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226239 representing NM_133649
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCCACCAGAAGCCACCACCAAGATGTCCTCAGTTCGGTTCATGGTGACACCAACTAAGATTGATG
  ACATTCCAGGTTTGTCAGACACCAGCCCGGACCTCAGCTCTCGGTCTAGTTCTCGAGTAAGATTTAGCTC
  CCGAGAAAGTGTGCCAGAAACAAGCCGTAGTGAGCCTATGAGCGAACTGTCTGGGGCTACTACTTCTCTG
  GCAACTGTTGCCCTAGATCCTTCCAGTGACCGGACTTCTAATCCCCAGGATGTTACGGAGGACCCGAGTC
  AGAACTCCATCACAGGGGAGCACAGCCAGCTGTTAGATGACGGCCATAAAAAAGCCCGAAATGCTTATCT
  CAATAATTCCAACTATGAAGAAGGAGACGAATATTTTGATAAAAATTTGGCACTCTTTGAGGAAGAAATG
  GACACCAGACCAAAGGTGTCTTCTCTCCTCAACCGCATGGCCAACTATACAAATCTGACACAAGGAGCAA
  AGGAACATGAAGAGGCAGAGAACATCACTGAAGGGAAAAAGAAGCCTACCAAGAGCCCCCAAATGGGTAC
  TTTCATGGGTGTCTACCTCCCATGTCTACAGAACATCTTTGGAGTGATCCTCTTCCTGCGTCTTACCTGG
  GTAGTGGGAACAGCTGGAATCCTTCAGGCCTTTGCAATTGTCCTCATCTGCTGCTGCTGTACAATGTTAA
  CTGCCATCTCCATGAGCGCCATCGCCACTAACGGAGTGGTGCCAGCTGGGGGCTCATACTTCATGATTTC
  CAGAGCCCTGGGCCCAGAGTTTGGCGGGGCTGTAGGCCTCTGCTTTTATCTTGGCACCACATTTGCAGCA
  GCCATGTATATTCTTGGTGCCATTGAAATCTTTCTGGTATACATTGTCCCCCGAGCTGCCATCTTTCGGA
  GTGACGATGCACTCAAGGAGTCAGCAGCTATGCTGAACAACATGCGCGTCTATGGTACAGCCTTCTTGGT
  CCTCATGGTCTTGGTGGTATTCATCGGCGTACGCTATGTGAATAAGTTTGCCTCACTCTTCCTGGCCTGT
  GTAATTGTGTCGATCTTGGCTATCTATGCTGGTGCCATCAAGTCTTCCTTTGCTCCACCACACTTCCCGG
  TCTGTATGCTGGGCAACCGTACCCTGTCATCAAGACACCTTGACATTTGCTCTAAGACCAAGGAGGTTGA
  CAACATGACAGTACCATCAAAGTTATGGGGATTCTTCTGCAACTCGAGTCAGTTCTTTAATGCCACCTGT
  GATGAGTACTTTGTTCACAATAACGTCATCTCAATCCAAGGCATTCCAGGGTTGGCTAGTGGTATCATTA
  CTGAAAATCTTTGGAGTAATTATTTACCAAAGGGTGAGATAATTGAAAAGCCATCAGCCAAGTCATCTGA
  TGTCTTAGGCAACTTAAACCATGAATATGTTCTTGCTGATATCACCACCTCCTTCACTCTGCTGGTGGGG
  ATCTTCTTTCCCTCGGTCACAGGTATCATGGCTGGGTCAAACAGATCTGGAGATCTGAAAGATGCCCAGA
  AGTCTATTCCCATTGGGACCATCCTTGCCATCCTGACCACATCCTTTGTGTATTTAAGCAATGTTGTCCT
  TTTTGGTGCATGTATTGAAGGAGTCGTTCTCAGAGACAAATTTGGGGATGCTGTAAAAGGGAATTTGGTT
  GTAGGCACCTTATCCTGGCCATCCCCGTGGGTGATCGTTATTGGCTCCTTCTTTTCAACATGTGGGGCTG
  GGCTGCAGAGCCTCACAGGTGCGCCTCGGCTGCTGCAGGCTATCGCCAAGGATAACATCATACCTTTCCT
  TAGGGTTTTTGGTCACAGCAAAGCTAATGGGGAACCTACCTGGGCTTTACTTCTAACTGCTGCCATAGCA
  GAGCTGGGAATTCTCATCGCCTCCCTGGATCTCGTGGCCCCAATTCTTTCCATGTTTTTTCTCATGTGTT
  ACCTCTTTGTGAACTTGGCTTGTGCCTTGCAAACATTGCTGCGAACCCCCAACTGGAGGCCTCGATTCCG
  CTATTATCACTGGGCCCTCTCTTTCATGGGAATGAGTATCTGTCTAGCTCTGATGTTCATTTCTTCTTGG
  TATTATGCCATTGTAGCTATGGTAATAGCTGGCATGATCTACAAGTACATTGAATATCAAGGGGCTGAGA
  AAGAATGGGGGGATGGTATCCGTGGGCTGTCGCTCAGTGCAGCCCGCTTCGCTTTGCTCCGTCTAGAGGA
  AGGACCTCCTCACACTAAAAACTGGAGGCCTCAGCTGCTCGTCCTACTGAAGCTGGATGAAGATTTACAC
  GTCAAGCACCCTCGCCTCCTCACCTTTGCCTCCCAGCTCAAGGCAGGAAAGGGACTCACGATTGTGGGCT
  CTGTCATCGTGGGGAACTTCTTGGAGAACTATGGTGACGCGCTCGCGGCAGAGCAGACCATTAAGCACCT
  AATGGAGGCAGAAAAGGTAAAAGGATTCTGCCAATTGGTGGTGGCTGCCAAGCTGAAAGAGGGCATTTCA
  CACCTCATCCAGTCCTGTGGCCTCGGAGGCATGAAACACAACACAGTGGTGATGGGCTGGCCCAATGGCT
  GGCGTCAGAGTGAAGATGCTCGCGCTTGGAAGACTTTCATTGGCACAGTACGAGTGACAACTGCTGCCCA
  TCTAGCCCTGCTGGTGGCTAAAAATGTCTCCTTCTTTCCCAGCAATGTGGAGCAGTTTTCTGAGGGCAAC
  ATTGATGTGTGGTGGATTGTGCATGATGGGGGCATGCTCATGCTATTACCGTTCCTGCTGAAACAGCACA
  AGGTTTGGCGGAAATGCAGCATACGGATCTTCACAGTAGCCCAACTAGAAGACAACAGTATCCAGATGAA
  GAAGGATCTGGCCACCTTTCTGTACCACCTGCGCATTGAGGCAGAAGTGGAAGTGGTGGAGATGCACGAC
  AGTGACATATCTGCCTATACATATGAGCGCACCCTGATGATGGAGCAGAGGTCCCAGATGCTTCGGCATA
  TGCGGCTGTCCAAAACAGAGCGAGACAGGGAGGCACAGCTGGTGAAAGATCGAAACTCAATGCTACGCTT
  GACCAGCATTGGCTCTGATGAGGACGAAGAGACAGAAACGTACCAGGAGAAGGTGCACATGACTTGGACC
  AAGGATAAATACATGGCATCCCGGGGGCAAAAGGTCAAGTCAATGGAAGGATTCCAGGACCTACTTAATA
  TGCGTCCGGACCAGTCCAACGTGAGACGGATGCATACAGCAGTGAAGCTCAATGAAGTTATAGTCAACAA
  GTCTCATGAAGCAAAGCTGGTTTTGTTGAATATGCCAGGACCACCCCGGAACCCTGAAGGTGATGAAAAC
  TACATGGAATTTCTAGAAGTGCTCACTGAGGGATTAGAACGAGTCCTTCTTGTCCGGGGTGGTGGCAGTG
  AGGTCATCACCATTTACTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226239 representing NM_133649
  Red=Cloning site Green=Tags(s)
  
  MHPPEATTKMSSVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSELSGATTSL
  ATVALDPSSDRTSNPQDVTEDPSQNSITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEM
  DTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTW
  VVGTAGILQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAA
  AMYILGAIEIFLVYIVPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLAC
  VIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHLDICSKTKEVDNMTVPSKLWGFFCNSSQFFNATC
  DEYFVHNNVISIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVG
  IFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLV
  VGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIA
  ELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSW
  YYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLH
  VKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGIS
  HLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGN
  IDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHD
  SDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWT
  KDKYMASRGQKVKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDEN
  YMEFLEVLTEGLERVLLVRGGGSEVITIYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133649
• ORF Size: 3450 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133649.2, NP_598410.2
• RefSeq Size: 6631 bp
• RefSeq ORF: 3453 bp
• Locus ID: 107723
• UniProt ID: Q924N4
• Cytogenetics: 2 E3
• MW: 128 kDa
• Gene Summary: Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.[UniProtKB/Swiss-Prot Function]