Pum2 (NM_001160221) Mouse Tagged ORF Clone

CAT#: MR226200

Pum2 (Myc-DDK-tagged) - Mouse pumilio 2 (Drosophila) (Pum2), transcript variant 4

Product Images

Circular map for MR226200

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pum2
• Synonyms: 5730503J23Rik; Pumm2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226200 representing NM_001160221
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCATGATTTTCAAGCTCTTGCATTAGAATCTCGGGGAATGGGAGAGCTTTTGCCTACCAAAAAGT
  TTTGGGAACCTGATGATTCAACAAAAGATGGACAAAAAGGCATATTTCTTGGGGATGATGAATGGAGAGA
  GACTGCATGGGGAACTTCTCATCATTCAATGTCCCAGCCTATTATGGTGCAGAGAAGATCTGGACAGAGT
  TTTCATGGAAACAGTGAAGTAAATGCAATTCTTTCTCCACGCTCAGAAAGTGGAGGCCTTGGTGTGAGCA
  TGGTAGAATATGTATTAAGTTCCTCTCCTGCTGATAAATTGGATTCTCGATTTAGGAAGGGAACTTTTGG
  TACTAGAGATGCTGAAACAGATGGACCTGAGAAAGGAGATCAAAAAGGCAAGGCTTCTCCATTTGAGGAG
  GACCAAAACAGAGATCTTAAACAAGATGATGAGGACTCTAAAATAAATGGCAGAGGTTTGCCAAATGGAA
  TGGATGCCGATTGCAAAGATTTTAATCGCACTCCTGGAAGTCGCCAAGCCTCTCCAACTGAAGTAGTTGA
  GCGCCTTGGCCCTAGTACTAATCCCCCAGAAGGATTGGGCCCTCTTCCTAATCCGACAGCGAATAAACCA
  CTTGTTGAAGAATTTTCAAACCCTGAAACTCAGAATCTGGATGCAATGGACCAAGTTGGTCTGGATTCCT
  TACAGTTTGACTATCCTGGTAATCAGGTACCCATGGATTCTTCAGGAGCTACTGTAGGCCTTTTTGACTA
  CAATTCCCAACAGCAGCTCTTTCAGAGGACTAGTGCACTAACAGTTCAGCAGTTAACTGCAGCTCAGCAG
  CAGCAGTATGCATTAGCAGCAGCTCAGCAGCCACATATAGCTGGTGTATTCTCAGCAGGCCTTGCTCCAG
  CTGCATTTGTGCCAAATCCATATATTATTAGTGCTGCTCCTCCAGGGACTGACCCGTATACTGCAGCAGG
  ATTGGCTGCAGCAGCAACATTAGCAGGTCCAGCAGTGGTTCCACCTCAGTATTACGGTGTTCCATGGGGA
  GTGTATCCAGCCAATTTATTTCAGCAACAAGCTGCAGCTGCGGCAAGCAACACAGCCAACCAGCAAGCAG
  CATCACAAGCTCAGCCTGGACAGCAGCAGGTTCTTCGTCCTGGAGCAGGTCAGCGTCCTATTACTCCAAG
  TCAGGGCCAACAAGGGCAGCAAGCAGAGTCACTTGCAGCAGCTGCAAACCCAACTTTGGCTTTTGGTCAG
  AGTCTTGCTGCAGGCATGCCAGGCTATCAAGTACTAGCTCCAACTGCCTATTATGATCAGACTGGTGCCT
  TAGTGGTTGGCCCCGGAGCAAGAACTGGCCTTGGAGCTCCAGTACGATTAATGGCTCCAACACCTGTCTT
  AATAAGTTCAACAGCAGCACAAGCTGCAGCAGCAGCAGCAGCAGCTGGAGGAACTGCAAATAGTCTTACA
  GGCAGCACAAATGGTCTGTTTCGGCCAATTGGCACTCAGCCACCACAGCAGCAGCAGCAGCAGCAGCAAC
  CAAGCACTAACCTGCAGTCTAATTCATTTTATGGGAGCAGCTCTTTGACTAACAGCTCCCAGAGCAGCTC
  TTTATTCTCTCATGGACCTGGCCAACCTGGAAGTGCGTCTCTTGGCTTTGGAAGTGGTAGCTCTTTAGGA
  GCTGCTATAGGCTCAGCTCTCAGTGGATTTGGCTCATCAGTTGGCAGTTCTGCAAGTAGTAGTGCCACAA
  GGAGAGAGTCTCTATCTACTAGCTCTGACTTGTACAAAAGATCTAGTAGCAGCCTAGCACCCATAGGGCA
  ACCATTTTACAATAGTCTGGGATTTTCCTCCTCTCCAAGTCCAATAGGCATGCCTCTGCCAAGCCAAACT
  CCAGGACATTCACTTACGCCACCGCCATCACTTTCATCACATGGATCCTCATCCAGTTTGCATTTAGGAG
  GACTGACAAACGGTAGTGGTCGGTATATCTCTGCAGCACCTGGAGCAGAAGCAAAGTACCGAAGTGCTTC
  AAGCACTTCCAGTCTATTTAGCTCCAGCAGCCAGCTCTTTCCTCCTTCTCGGCTCCGCTATAATAGATCT
  GACATCATGCCCTCCGGCCGCAGTAGGTTATTGGAAGATTTCAGGAACAACCGCTTCCCCAACCTTCAGC
  TCAGAGACTTAATTGGACATATAGTCGAGTTTTCTCAAGACCAGCATGGTTCCAGATTCATACAGCAAAA
  GCTAGAGAGAGCTACTCCAGCTGAGCGACAAATAGTATTTAATGAAATTCTACAGGCAGCCTATCAATTA
  ATGACAGATGTTTTTGGAAACTATGTTATACAGAAGTTTTTTGAGTTTGGAAGTTTGGATCAGAAATTAG
  CCCTGGCTACTCGTATTCGTGGTCATGTTCTACCATTAGCCTTGCAGATGTATGGCTGCCGTGTTATTCA
  AAAGGCGTTAGAATCTATTTCTTCTGATCAGCAGAGTGAAATGGTTAAGGAACTAGATGGCCATGTACTT
  AAATGTGTGAAAGATCAAAATGGAAACCATGTTGTACAGAAATGCATTGAATGTGTTCAGCCACAGTCAC
  TGCAGTTCATCATCGATGCTTTCAAAGGACAAGTATTTGTGCTTTCAACCCATCCTTATGGCTGCAGAGT
  CATTCAGCGTATCTTAGAGCACTGCACGGCAGAGCAGACCTTACCCATCTTAGAAGAACTTCACCAACAC
  ACAGAACAGTTGGTACAGGATCAGTATGGCAATTATGTTATTCAGCATGTACTGGAACATGGTCGACCTG
  AAGACAAGAGCAAAATTGTTTCCGAAATCAGAGGAAAGGTCTTAGCCCTGAGTCAACACAAATTTGCCAG
  CAATGTAGTAGAAAAGTGTGTTACTCATGCCTCCCGTGCTGAGAGAGCTTTACTGATTGATGAGGTCTGC
  TGTCAGAATGATGGTCCTCACAGTGCCTTATACACCATGATGAAGGATCAGTATGCCAACTATGTGGTTC
  AGAAGATGATTGATATGGCTGAGCCTGCGCAGAGAAAGATAATCATGCACAAGATTCGACCTCATATTAC
  TACTCTTCGCAAATACACATATGGGAAGCATATACTGGCCAAGTTGGAAAAATACTATCTGAAAAACAGC
  CCAGATCTAGGGCCAATTGGAGGACCACCAAATGGGATGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226200 representing NM_001160221
  Red=Cloning site Green=Tags(s)
  
  MNHDFQALALESRGMGELLPTKKFWEPDDSTKDGQKGIFLGDDEWRETAWGTSHHSMSQPIMVQRRSGQS
  FHGNSEVNAILSPRSESGGLGVSMVEYVLSSSPADKLDSRFRKGTFGTRDAETDGPEKGDQKGKASPFEE
  DQNRDLKQDDEDSKINGRGLPNGMDADCKDFNRTPGSRQASPTEVVERLGPSTNPPEGLGPLPNPTANKP
  LVEEFSNPETQNLDAMDQVGLDSLQFDYPGNQVPMDSSGATVGLFDYNSQQQLFQRTSALTVQQLTAAQQ
  QQYALAAAQQPHIAGVFSAGLAPAAFVPNPYIISAAPPGTDPYTAAGLAAAATLAGPAVVPPQYYGVPWG
  VYPANLFQQQAAAAASNTANQQAASQAQPGQQQVLRPGAGQRPITPSQGQQGQQAESLAAAANPTLAFGQ
  SLAAGMPGYQVLAPTAYYDQTGALVVGPGARTGLGAPVRLMAPTPVLISSTAAQAAAAAAAAGGTANSLT
  GSTNGLFRPIGTQPPQQQQQQQQPSTNLQSNSFYGSSSLTNSSQSSSLFSHGPGQPGSASLGFGSGSSLG
  AAIGSALSGFGSSVGSSASSSATRRESLSTSSDLYKRSSSSLAPIGQPFYNSLGFSSSPSPIGMPLPSQT
  PGHSLTPPPSLSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSASSTSSLFSSSSQLFPPSRLRYNRS
  DIMPSGRSRLLEDFRNNRFPNLQLRDLIGHIVEFSQDQHGSRFIQQKLERATPAERQIVFNEILQAAYQL
  MTDVFGNYVIQKFFEFGSLDQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQSEMVKELDGHVL
  KCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQH
  TEQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDEVC
  CQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEKYYLKNS
  PDLGPIGGPPNGML
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001160221
• ORF Size: 3192 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001160221.1, NP_001153693.1
• RefSeq Size: 6120 bp
• RefSeq ORF: 3195 bp
• Locus ID: 80913
• Cytogenetics: 12 A1.1
• MW: 114.6 kDa
• Gene Summary: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3' UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3' UTR and facilitating miRNA regulation. Plays a role in cytoplasmic sensing of viral infection. Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm. May regulate DCUN1D3 mRNA levels. May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level (By similarity).[UniProtKB/Swiss-Prot Function]