Egfr (NM_207655) Mouse Tagged ORF Clone
Lenti ORF clone of Egfr (Myc-DDK-tagged) - Mouse epidermal growth factor receptor (Egfr), transcript variant 1
|Type||Mouse Tagged ORF Clone|
|Synonyms||9030024J15Rik; AI552599; Erbb; Errb1; Errp; wa-2; wa2; Wa5|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||3630 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||5983 bp|
|RefSeq ORF||3633 bp|
|Cytogenetics||11 9.41 cM|
|Gene Summary||Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:8404850). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (By similarity). Plays a role in enhancing learning and memory performance (PubMed:20639532).[UniProtKB/Swiss-Prot Function]|
|cDNA Clone Resources|
|MG226160||Egfr (GFP-tagged) - Mouse epidermal growth factor receptor (Egfr) transcript variant 1, (10ug)||
|MR226160||Egfr (Myc-DDK-tagged) - Mouse epidermal growth factor receptor (Egfr), transcript variant 1||
|MR226160L1||Lenti ORF clone of Egfr (Myc-DDK-tagged) - Mouse epidermal growth factor receptor (Egfr), transcript variant 1||
|MR226160L2||Lenti ORF clone of Egfr (mGFP-tagged) - Mouse epidermal growth factor receptor (Egfr), transcript variant 1||
|MR226160L4||Lenti ORF clone of Egfr (mGFP-tagged) - Mouse epidermal growth factor receptor (Egfr), transcript variant 1||