Epha3 (NM_010140) Mouse Tagged ORF Clone

CAT#: MR226138

Epha3 (Myc-DDK-tagged) - Mouse Eph receptor A3 (Epha3)

Product Images

Circular map for MR226138

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Epha3
• Synonyms: AW492086; Cek4; End3; ETK1; Hek; Hek4; Mek4; Tyro4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226138 representing NM_010140
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTGTCACCTCTCCATCCTCGTCCTGCTCGGCTGCTGTGTCCTCAGCTGCTCCGGAGAACTGAGCC
  CACAGCCTTCCAACGAAGTTAATCTACTAGATTCGAAAACAATTCAAGGAGAGCTGGGCTGGATCTCCTA
  CCCATCCCATGGGTGGGAAGAGATCAGTGGTGTTGATGAACATTACACACCAATCAGGACTTACCAGGTG
  TGCAATGTCATGGATCACAGCCAAAATAATTGGCTGAGGACAAACTGGGTACCCAGAAACTCAGCTCAGA
  AGATCTATGTGGAGCTAAAGTTCACACTGCGGGACTGTAACAGCATTCCATTGGTTTTGGGGACTTGCAA
  GGAGACCTTTAACCTGTACTACATGGAGTCTGATGATGATCATGGCGTCAAATTCCGAGAGCATCAGTTC
  ACGAAGATTGACACCATTGCCGCTGATGAAAGTTTCACTCAGATGGATCTCGGGGATCGCATTCTGAAAC
  TCAACACTGAGATTAGAGAAGTGGGACCAGTCAACAAAAAGGGGTTTTATTTGGCCTTTCAAGATGTTGG
  TGCTTGTGTTGCCTTGGTGTCTGTGAGAGTGTACTTCAAAAAGTGCCCGTTTACAGTGAAGAATCTGGCT
  ATGTTTCCAGACACAGTGCCCATGGACTCCCAGTCTTTGGTGGAGGTTAGGGGCTCTTGTGTCAATAATT
  CCAAAGAGGAGGACCCTCCCAGGATGTACTGCAGCACAGAAGGGGAATGGCTGGTCCCCATTGGCAAATG
  CACTTGCAATGCTGGGTATGAAGAACGAGGTTTCATATGCCAAGCTTGTCGACCAGGCTTCTACAAGGCT
  TCCGATGGTGCTGCTAAGTGTGCTAAGTGCCCACCACACAGCTCAACACAAGAAGATGGTTCAATGAACT
  GCAGGTGTGAGAATAATTATTTCCGGGCAGAAAAAGACCCTCCATCCATGGCTTGTACCCGACCTCCATC
  CGCACCAAGAAATGTTATCTCAAACATAAACGAGACCTCGGTTATCCTGGACTGGAGTTGGCCCCTGGAC
  ACAGGAGGCCGGAAGGATATTACCTTCAACATCATATGTAAAAAGTGTGGGTGGAATGTCAGGCAGTGTG
  AGCCGTGCAGCCCCAACGTCCGCTTCCTTCCTCGGCAGCTTGGACTCACCAACACCACGGTGACAGTGAC
  AGACCTCCTAGCTCACACCAACTACACCTTTGAGATCGATGCTGTTAATGGGGTGTCAGAGCTGAGCTCG
  CCACCGAGACAGTATGCCGCAGTCAGCATCACAACTAATCAGGCTGCTCCATCACCAGTTATGACAATTA
  AGAAAGATCGGACTTCCAGAAACAGTATCTCTTTATCTTGGCAAGAACCGGAACATCCAAATGGAATTAT
  CCTGGACTATGAGGTCAAATACTATGAAAAGCAGGAGCAAGAGACGAGTTATACCATTTTGAGAGCAAGA
  GGCACAAATGTTACTATCAGTAGCCTCAAGCCAGATACTACTTATGTCTTCCAAATCCGAGCTCGGACAG
  CAGCGGGATATGGAACCAACAGCCGCAAATTTGAATTTGAAACTAGCCCAGACTCTTTCTCCATCTCCGG
  TGAAAACAGTCATGTGGTGATGATTGCTATTTCCGCAGCTGTGGCCATCATTGTCCTCACGGTGGTCACC
  TATGTTCTGGTCGGGAGGTTTTGTGGCTATCACAAGTCAAAACACAGTGCAGAGGAGAAGCGCCTTCACT
  TTGGGAATGGCCACTTAAAACTTCCAGGTCTCAGGACCTATGTTGATCCACATACATATGAAGACCCTAC
  TCAAGCAGTTCATGAGTTCGCGAAGGAGTTGGATGCCACCAATATATCCATCGACAAAGTTGTTGGAGCA
  GGTGAATTTGGAGAAGTTTGCAGCGGCCGCTTAAAACTTCCTTCAAAAAAAGAGATTTCGGTGGCCATAA
  AGACCCTGAAAGTAGGGTATACCGAGAAACAGAGGAGAGACTTCCTCGGTGAAGCGAGCATCATGGGACA
  GTTTGACCATCCCAACATCATCCGACTGGAAGGCGTTGTCACTAAGAGTAAGCCAGTGATGATTGTCACG
  GAATACATGGAGAATGGCTCCTTGGACAGTTTCTTACGTAAGCACGATGCCCAGTTCACAGTCATCCAGC
  TTGTGGGAATGCTCCGTGGGATAGCATCAGGTATGAAATACCTCTCAGATATGGGCTACGTCCACCGGGA
  CCTTGCTGCTCGCAACATCTTGATCAACAGTAACTTAGTGTGTAAAGTTTCTGACTTTGGACTCTCTCGG
  GTACTTGAAGATGACCCAGAAGCTGCTTACACAACCAGGGGAGGAAAGATACCAATAAGGTGGACATCAC
  CAGAAGCAATTGCCTACCGCAAGTTCACCTCAGCCAGCGATGTATGGAGTTACGGGATTGTTCTCTGGGA
  AGTGATGTCTTACGGAGAAAGGCCATACTGGGAGATGTCCAATCAGGATGTAATTAAGGCTGTGGATGAG
  GGCTATCGCCTGCCACCTCCCATGGATTGCCCAGCTGCCTTGTATCAGTTGATGTTGGACTGCTGGCAGA
  AAGACAGGAACAACAGACCCAAGTTCGAGCAGATCGTCAGCATTCTGGACAAGCTCATCCGGAATCCAGG
  CAGTCTGAAGATCATCACCAGCGCGGCTGCAAGGCCATCAAACCTTCTTCTGGACCAAAGCAATGTCGAT
  ATCGCTACCTTCCACACAACTGGTGATTGGCTTAACGGCATGAGGACAGCACACTGTAAGGAAATCTTCA
  CAGGCGTCGAATACAGCTCCTGTGACACCATTGCCAAGATCTCCACAGATGACATGAAAAAGGTTGGTGT
  CACTGTGGTTGGGCCACAGAAGAAGATCATCAGCACCATTAAAGCTCTAGAAACACAATCTAAGAATGGT
  CCAGTTCCAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226138 representing NM_010140
  Red=Cloning site Green=Tags(s)
  
  MDCHLSILVLLGCCVLSCSGELSPQPSNEVNLLDSKTIQGELGWISYPSHGWEEISGVDEHYTPIRTYQV
  CNVMDHSQNNWLRTNWVPRNSAQKIYVELKFTLRDCNSIPLVLGTCKETFNLYYMESDDDHGVKFREHQF
  TKIDTIAADESFTQMDLGDRILKLNTEIREVGPVNKKGFYLAFQDVGACVALVSVRVYFKKCPFTVKNLA
  MFPDTVPMDSQSLVEVRGSCVNNSKEEDPPRMYCSTEGEWLVPIGKCTCNAGYEERGFICQACRPGFYKA
  SDGAAKCAKCPPHSSTQEDGSMNCRCENNYFRAEKDPPSMACTRPPSAPRNVISNINETSVILDWSWPLD
  TGGRKDITFNIICKKCGWNVRQCEPCSPNVRFLPRQLGLTNTTVTVTDLLAHTNYTFEIDAVNGVSELSS
  PPRQYAAVSITTNQAAPSPVMTIKKDRTSRNSISLSWQEPEHPNGIILDYEVKYYEKQEQETSYTILRAR
  GTNVTISSLKPDTTYVFQIRARTAAGYGTNSRKFEFETSPDSFSISGENSHVVMIAISAAVAIIVLTVVT
  YVLVGRFCGYHKSKHSAEEKRLHFGNGHLKLPGLRTYVDPHTYEDPTQAVHEFAKELDATNISIDKVVGA
  GEFGEVCSGRLKLPSKKEISVAIKTLKVGYTEKQRRDFLGEASIMGQFDHPNIIRLEGVVTKSKPVMIVT
  EYMENGSLDSFLRKHDAQFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILINSNLVCKVSDFGLSR
  VLEDDPEAAYTTRGGKIPIRWTSPEAIAYRKFTSASDVWSYGIVLWEVMSYGERPYWEMSNQDVIKAVDE
  GYRLPPPMDCPAALYQLMLDCWQKDRNNRPKFEQIVSILDKLIRNPGSLKIITSAAARPSNLLLDQSNVD
  IATFHTTGDWLNGMRTAHCKEIFTGVEYSSCDTIAKISTDDMKKVGVTVVGPQKKIISTIKALETQSKNG
  PVPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010140
• ORF Size: 2952 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010140.1, NM_010140.2, NM_010140.3, NP_034270.1
• RefSeq Size: 3978 bp
• RefSeq ORF: 2955 bp
• Locus ID: 13837
• Cytogenetics: 16 C1.3
• MW: 110.5 kDa