Wdr19 (NM_153391) Mouse Tagged ORF Clone

CAT#: MR226133

Wdr19 (Myc-DDK-tagged) - Mouse WD repeat domain 19 (Wdr19)

Product Images

Circular map for MR226133

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wdr19
• Synonyms: C330027H04Rik; D330023L08Rik; DYF2; Ift144; mKIAA1638; PWDMP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226133 representing NM_153391
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGTGTTTTCTCCCTGCTAGAAAAGTCTTGGCTTGGTGCTCCGATACAATTTGCCTGGCAAAAAT
  CATCAGGAAACTACCTTGCAGTAACAGGAGCTGATTATATTGTTAAAATCTTTGATCGCCATGGCCAAAA
  AAGAAGTGAAATTAGCTTGCCTGGCAACTGTGTTACCATGGATTGGGATAAAGATGGCGATATCCTGGCA
  GTGATTGCTGAGAAGTCTAGTTGCATTTATCTATGGGATGCCAACACAAATAAAACCAGCCAGCTGGACA
  ATGGCATGAGGGATCAAATGTCTTTCCTTCTTTGGTCAAAAATTGGAAGTTTCCTGGCTGTTGGGACCAT
  TAAAGGAAATTTGCTCATTTATAATCATCAGACATCTCGAAAGATTCCTGTTCTTGGAAAACATACTAAG
  AAAATCACATGTGGATGTTGGAATTCAGAGAATCTCCTTGCTTTGGGAGGTGAAGATAAAATGATTACAG
  TTAGTAACCAGGAAGGCGACACAATAAGACAGACCCCAGTGAAATCAGAGCCAAGCGACATCAAGTTCTC
  CATGAGCAAGACAGATGAGCGAATTTCTTCTGCTGAGAACACAATAAGTGCAGTGGTTGGCAAGAAAATG
  CTGTTTCTTTTTCATCTGAATGAACCAGATAACCCGGTGGATCTGGAGTTTCAGCAAGCCTATGGCAACA
  TTGTCTGCTATAGTTGGTATGGAGATGGCTACATCATGATTGGCTTTTCCCGAGGGACGTTTTTGGCTAT
  TTCTACTCACTTTCCGGAAGTTGGGCAAGAGATATTTAAGGCTCGTGACCATAAGGATAATCTAACCAGT
  GTGGCCTTGTCACAGACTCTGAACAAAGCTGCCACATGTGGCGATAACTGCATAAAAATCCATGATCTGA
  CAGAATTGAGAGACATGTATGCTATAATTAATCTGGATGATGAGAATAAAGGGCTGGGTACCTTATCCTG
  GACTGATGATGGTCAGTTGCTAGCACTGTCTACCCAAAGAGGCTCACTGCATGTCTTCCTGACCAAGTTG
  CCCATCCTCGGGGACGCCTGTCACACAAGGATTGCGTATCTCACCTCCCTCCTTGAGGTCACCGTGGCCA
  ACCTCATTGAAGGAGAGCCGCCAATCACAGTCTCTGTGGATGTGGAACCCACCTTTGTCGCAGTAGGGCT
  CTATCATCTGGCCGTGGGGATGAATAACCGGGCTTGGTTTTATGTCCTTGGTGAAAATGTTGTCAAAAAG
  TTAAAAGATGTGGAATATCTGGGAACCGTGGCCAGCATCTGCCTTCATTCTGACTACGCCGCTGCACTCT
  TTGAAGGCAAAATCCAGTTACATTTGATAGAAAATGAAATGTTGGACGCTCAGGAAGAGCGTGAGACTCG
  GCTCTTTCCAGCAGTGGATGATAAGTGCCGGATTTTATGCCACGCCCTAACTAGTGATTTCCTCATCTAC
  GGAACTGATACTGGCATCATTCACTATTTCTTCATCGAAGACTGGCAGTTCGTTAATGATTACCGGCATC
  CTGTTGGTGTGAAGAAGCTATTTCCTGATCCAAATGGAACCAGATTGGTTTTCATTGATGAGAAGAGTGA
  TGGATTTGTTTACTGTCCTGTTAATGATGCGACCTATGAGATTCCAGACTTCTCACCAACCATTAAAGGT
  GTTCTTTGGGAAAACTGGCCGATGGACAAAGGTGTCTTTATCGCCTATGATGATGACAAGGTGTATACAT
  ATGCGTTTCACAAGGACACCATCCAAGGATCCAAGGTTATTTTGGCTGGCAGCACCAAACTTCCCTTCTC
  CCATAAGCCTTTGCTGTTATACAATGGAGAACTGACCTGCCAGACACAGAGTGGGAAAATCAACTCCATC
  TACCTCAGCACCCACAGCTTCCTTGGCAGCATGAAAGACACGGAGCCTACTGACCTGAGGCAAATGCTGA
  CGCAGACCCTGCTGCTCAAGCGGTTTTCTGATGCTTGGGATATATGCAAGATGCTAAATGACCGCACTTC
  GTGGAGTGAGCTGGCCAAAGCCTGTCTGCATCACATGGAGGTGGAGTTTGCTATCCGAGTGTCCCGGACA
  ATGGGGGATGTTGGCACAGTGATGTCGTTGGAACAAATAAAGGGAATCGAGGACTACAATCTTTTGGCAG
  GACATCTCGCCATGTTTACTAATGACTTCAACCTGGCCCAGGACCTGTACCTGGCATCCAACTGCCCTGT
  GGCAGCCCTGGAGATGCGGCGGGACCTGCAGCACTGGGACAGCGCTCTGCAGCTGGCAAAGCGCCTGGCC
  CCGGACCAGATACCCTTCATATCCAAAGAGTACGCCATCCAGCTGGAGTTCACAGGCGATTATGTAAACG
  CTCTGGCTCATTACGAGAAGGGCATCACCGGTGATAATAAGGAACACGACGAAGTGTGCCTGGCCGGAGT
  GGCTCAGATGTCCATTCGAATGGGGGACATCCGCAGAGGGGCTAACCAAGCCCTCAAGCACCCCAGCAGG
  GTCCTCAAAAGAGACTGTGGAGCCATTCTGGAGAACATGAAGCAATTTTCAGAAGCTGCCCAGCTGTACG
  AAAAGGGCCAATATTATGACAGAGCTGCCTCGGTCTACATCCGCTGCAAGAACTGGGCAAAAGTTGGCGA
  ACTTCTCCCTCATGTCTCCTCTCCTAAGATCCACTTGCAGTATGCCAAAGCCAAGGAGGCAGACGGAAGG
  TACAAGGAAGCCGTGGTGGCGTATGAAAATGCAAAGCAATGGAACAGTGTCATCCGCATCTACCTGGACC
  ACCTCAACAACCCCGAAAAGGCCGTGAGCATCGTCAGAGAGACCCAGTCTCTGGACGGAGCCAAGATGGT
  AGCCAGGTTCTTTCTGCAGCTTGGTGACTATGGGTCTGCCATCCAGTTTCTGGTTCTGTCCAAATGTAAC
  AATGAAGCCTTCACCTTGGCTCAGCAGCACAACAAAATGGAAATCTACGCAGACATCATTGGTGCTGAAG
  ACACAACTAATGAAGACTATCAAAGTATCGCCTTATATTTTGAAGGAGAAAAAAGACATTTTCAGGCTGG
  AAAATTCTTCTTACTGTGTGGCCAGTATTCACGGGCACTAAAGCACTTCCTGAAATGCCCAAGCTCAGAA
  GATAATGTGGCAATAGAAATGGCAATCGAAACTGTGGGCCAGGCCAAAGATGAACTGCTGACCAATCAGC
  TGATCGACCACCTGATGGGGGAGAGCGATGGCATGCCAAAGGACGCCAAGTACCTGTTCCGCTTGTACAT
  GGCGCTAAAGCAGTACCGTGAAGCAGCCCGGACCGCCATCATCATCGCCAGAGAAGAGCAGTCTGCAGGA
  AACTATCGGAATGCACACGATGTTCTTTTCAGTATGTACGCAGAACTTAAAGCCCAGAAGATCAAGATCC
  CCTCCGAAATGGCCACCAACCTCATGATCCTGCACAGTTACATTCTCGTGAAGATTCATGTTAAGAGTGG
  AGACCATATGAAGGGAGCGCGCATGCTCATTCGGGTGGCCAACAATATCAGCAAGTTCCCATCACACATC
  GTGCCTATCCTGACGTCTACTGTGATTGAGTGTCATAGGGCAGGCCTGAAAAACTCTGCCTTCAGCTTTG
  CAGCTATGCTGATGAGGCCTGAATACCGCAACAAAATTGATGCCAAGTACAAAAAGAAAATTGAGGCGAT
  GGTCAGGAGACCCGATACTTCAGAGACAGAAGAGGCCACCACCCCATGTCCATTCTGCCAGTTTCTTCTC
  CCAGAATGTGAGCTCCTCTGTCCTGGCTGTAAAAACAACATTCCCTATTGCATTGCAACAGGCCGACACA
  TGTTGAAAGACGACTGGACAATGTGCCCGCATTGTGGCTTCCCTGCTCTGTACTCAGAATTCAAGATCTT
  ACTAAACAGTGAAAGCACGTGTCCTATGTGTTCAGAAAGATTAAACTCCAGTCAACTGAAAAAAATTACA
  GACTGCTCGCAGTACCTACGGACAGAGATGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226133 representing NM_153391
  Red=Cloning site Green=Tags(s)
  
  MKRVFSLLEKSWLGAPIQFAWQKSSGNYLAVTGADYIVKIFDRHGQKRSEISLPGNCVTMDWDKDGDILA
  VIAEKSSCIYLWDANTNKTSQLDNGMRDQMSFLLWSKIGSFLAVGTIKGNLLIYNHQTSRKIPVLGKHTK
  KITCGCWNSENLLALGGEDKMITVSNQEGDTIRQTPVKSEPSDIKFSMSKTDERISSAENTISAVVGKKM
  LFLFHLNEPDNPVDLEFQQAYGNIVCYSWYGDGYIMIGFSRGTFLAISTHFPEVGQEIFKARDHKDNLTS
  VALSQTLNKAATCGDNCIKIHDLTELRDMYAIINLDDENKGLGTLSWTDDGQLLALSTQRGSLHVFLTKL
  PILGDACHTRIAYLTSLLEVTVANLIEGEPPITVSVDVEPTFVAVGLYHLAVGMNNRAWFYVLGENVVKK
  LKDVEYLGTVASICLHSDYAAALFEGKIQLHLIENEMLDAQEERETRLFPAVDDKCRILCHALTSDFLIY
  GTDTGIIHYFFIEDWQFVNDYRHPVGVKKLFPDPNGTRLVFIDEKSDGFVYCPVNDATYEIPDFSPTIKG
  VLWENWPMDKGVFIAYDDDKVYTYAFHKDTIQGSKVILAGSTKLPFSHKPLLLYNGELTCQTQSGKINSI
  YLSTHSFLGSMKDTEPTDLRQMLTQTLLLKRFSDAWDICKMLNDRTSWSELAKACLHHMEVEFAIRVSRT
  MGDVGTVMSLEQIKGIEDYNLLAGHLAMFTNDFNLAQDLYLASNCPVAALEMRRDLQHWDSALQLAKRLA
  PDQIPFISKEYAIQLEFTGDYVNALAHYEKGITGDNKEHDEVCLAGVAQMSIRMGDIRRGANQALKHPSR
  VLKRDCGAILENMKQFSEAAQLYEKGQYYDRAASVYIRCKNWAKVGELLPHVSSPKIHLQYAKAKEADGR
  YKEAVVAYENAKQWNSVIRIYLDHLNNPEKAVSIVRETQSLDGAKMVARFFLQLGDYGSAIQFLVLSKCN
  NEAFTLAQQHNKMEIYADIIGAEDTTNEDYQSIALYFEGEKRHFQAGKFFLLCGQYSRALKHFLKCPSSE
  DNVAIEMAIETVGQAKDELLTNQLIDHLMGESDGMPKDAKYLFRLYMALKQYREAARTAIIIAREEQSAG
  NYRNAHDVLFSMYAELKAQKIKIPSEMATNLMILHSYILVKIHVKSGDHMKGARMLIRVANNISKFPSHI
  VPILTSTVIECHRAGLKNSAFSFAAMLMRPEYRNKIDAKYKKKIEAMVRRPDTSETEEATTPCPFCQFLL
  PECELLCPGCKNNIPYCIATGRHMLKDDWTMCPHCGFPALYSEFKILLNSESTCPMCSERLNSSQLKKIT
  DCSQYLRTEME
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153391
• ORF Size: 4023 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153391.2, NP_700440.2
• RefSeq Size: 4403 bp
• RefSeq ORF: 4026 bp
• Locus ID: 213081
• UniProt ID: Q3UGF1
• Cytogenetics: 5 C3.1
• MW: 151.5 kDa
• Gene Summary: As component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is involved in cilia function and/or assembly (Probable). Essential for functional IFT-A assembly and ciliary entry of GPCRs (By similarity). Associates with the BBSome complex to mediate ciliary transport (PubMed:22922713).[UniProtKB/Swiss-Prot Function]