Fzd5 (NM_022721) Mouse Tagged ORF Clone

CAT#: MR226069

Fzd5 (Myc-DDK-tagged) - Mouse frizzled homolog 5 (Drosophila) (Fzd5), transcript variant 1

Product Images

Circular map for MR226069

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fzd5
• Synonyms: 5330434N09Rik; AI427138; Fz-5; Fz5; mFz5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226069 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGACCCGACCCGTCTGCGCCTCCCTCTCTTCTGCTGCTGTTGCTGGCGCAGCTGGTGGGCCGGG
  CAGCGGCCGCCTCCAAGGCCCCGGTGTGCCAGGAAATCACGGTGCCCATGTGCCGAGGCATCGGCTACAA
  CCTGACGCACATGCCCAACCAGTTCAACCATGACACGCAGGACGAAGCAGGCCTGGAGGTGCACCAATTC
  TGGCCGCTTGTGGAGATCCACTGCTCACCGGACCTGCGCTTCTTCCTGTGCTCTATGTACACGCCCATCT
  GTTTGCCTGACTACCACAAGCCGCTACCACCGTGCCGTTCCGTGTGCGAGCGCGCCAAGGCCGGCTGCTC
  GCCGCTCATGCGCCAGTACGGCTTCGCCTGGCCCGAGCGCATGAGCTGCGACCGCCTCCCTGTGCTGGGC
  GGCGACGCCGAGGTTCTGTGTATGGATTATAACCGAAGCGAAGCCACCACCGCGTCCCCTAAGTCCTTCC
  CGGCCAAACCTACACTCCCAGGACCACCAGGGGCGCCATCTTCCGGGGGCGAGTGCCCCTCGGGAGGCCC
  ATCCGTGTGCACGTGCCGCGAGCCCTTCGTGCCCATCCTGAAGGAGTCACACCCACTCTACAACAAGGTG
  CGCACCGGCCAAGTGCCCAACTGCGCGGTGCCCTGCTACCAGCCGTCCTCCAGCCCGGACGAGCGCACAT
  TCGCCACCTTCTGGATTGGCCTGTGGTCTGTGCTGTGCTTCATCTCCACGTCCACCACCGTTGCCACCTT
  CCTCATTGACATGGAACGATTCCGCTACCCTGAGCGCCCCATCATCTTCTTGTCTGCGTGCTACCTGTGT
  GTGTCACTGGGATTCTTGGTGCGCCTGGTAGTGGGCCATGCCAGCGTCGCTTGCAGCCGTGAGCACAGCC
  ACATTCACTATGAGACTACCGGCCCTGCGCTGTGCACGGTTGTCTTCCTCTTAGTCTATTTCTTTGGCAT
  GGCCAGCTCCATCTGGTGGGTCATCCTGTCGCTCACCTGGTTCTTGGCGGCTGGCATGAAGTGGGGCAAT
  GAAGCCATCGCAGGTTATGCACAGTACTTCCACCTTGCTGCCTGGCTCATCCCCAGTGTCAAGTCCATTA
  CGGCGCTGGCACTGAGCTCGGTGGACGGGGACCCAGTGGCTGGCATCTGCTATGTGGGCAACCAAAACCT
  GAACTCACTACGAGGCTTTGTCTTGGGCCCACTGGTGCTGTACCTGTTGGTGGGCACGCTCTTCCTTCTG
  GCAGGCTTCGTGTCACTCTTCCGCATCCGGAGCGTCATCAAGCAGGGTGGCACTAAGACGGACAAGCTAG
  AGAAGCTCATGATCCGCATCGGCATCTTCACCCTGCTCTACACGGTGCCAGCCAGCATCGTGGTGGCCTG
  CTACCTGTATGAGCAGCACTACCGGGAGAGCTGGGAGGCAGCCCTCACCTGCGCGTGTCCGGGACCGGAC
  GCTGGCCAGCCACGCGCCAAACCCGAGTACTGGGTGCTCATGCTCAAGTACTTCATGTGCCTGGTGGTGG
  GCATCACGTCGGGAGTCTGGATCTGGTCCGGCAAGACTCTGGAGTCTTGGCGGCGGTTCACCAGCCGCTG
  CTGCTGCAGCTCTCGGCGGGGCCACAAGAGCGGTGGCGCTATGGCCGCAGGAGACTATGCGGAGGCCAGC
  GCCGCGCTCACCGGCAGGACCGGGCCGCCTGGCCCCACCGCCGCATACCACAAGCAAGTGTCCCTGTCGC
  ACGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226069 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARPDPSAPPSLLLLLLAQLVGRAAAASKAPVCQEITVPMCRGIGYNLTHMPNQFNHDTQDEAGLEVHQF
  WPLVEIHCSPDLRFFLCSMYTPICLPDYHKPLPPCRSVCERAKAGCSPLMRQYGFAWPERMSCDRLPVLG
  GDAEVLCMDYNRSEATTASPKSFPAKPTLPGPPGAPSSGGECPSGGPSVCTCREPFVPILKESHPLYNKV
  RTGQVPNCAVPCYQPSSSPDERTFATFWIGLWSVLCFISTSTTVATFLIDMERFRYPERPIIFLSACYLC
  VSLGFLVRLVVGHASVACSREHSHIHYETTGPALCTVVFLLVYFFGMASSIWWVILSLTWFLAAGMKWGN
  EAIAGYAQYFHLAAWLIPSVKSITALALSSVDGDPVAGICYVGNQNLNSLRGFVLGPLVLYLLVGTLFLL
  AGFVSLFRIRSVIKQGGTKTDKLEKLMIRIGIFTLLYTVPASIVVACYLYEQHYRESWEAALTCACPGPD
  AGQPRAKPEYWVLMLKYFMCLVVGITSGVWIWSGKTLESWRRFTSRCCCSSRRGHKSGGAMAAGDYAEAS
  AALTGRTGPPGPTAAYHKQVSLSHV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022721
• ORF Size: 1758 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022721.2, NM_022721.3, NP_073558.2
• RefSeq Size: 6930 bp
• RefSeq ORF: 1758 bp
• Locus ID: 14367
• UniProt ID: Q9EQD0
• Cytogenetics: 1 32.74 cM
• MW: 64.1 kDa
• Gene Summary: Receptor for Wnt proteins (PubMed:11092808, PubMed:18230341). Can activate WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 (in vitro); the in vivo situation may be different since not all of these are known to be coexpressed (PubMed:11092808). In neurons, activation of WNT7A promotes formation of synapses (By similarity). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:18230341). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (PubMed:18230341). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization (PubMed:11092808).[UniProtKB/Swiss-Prot Function]