Ntrk1 (NM_001033124) Mouse Tagged ORF Clone

CAT#: MR226042

Ntrk1 (Myc-DDK-tagged) - Mouse neurotrophic tyrosine kinase, receptor, type 1 (Ntrk1)

Product Images

Circular map for MR226042

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ntrk1
• Synonyms: C80751; Tkr; trk; TrkA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226042 representing NM_001033124
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGAGGCCAGCGGCTCGGGCAGCTGGGCTGGCATCGCCCGGCCGCGGGGCTAGGCAGTCTGATGA
  CTTCGTTGATGCTGGCCTGTGCCTCCGCCGCATCCTGTCGCGAGGTCTGCTGTCCCGTGGGCCCCTCGGG
  GCTGCGCTGCACCAGGGCAGGGTCCCTGGATACCCTCCGCGGCCTGCGGGGCGCCGGGAACCTGACGGAG
  CTCTACGTGGAAAACCAGCAGCACCTGCAACGCTTGGAGTTTGAGGACCTGCAGGGCCTGGGGGAGTTGA
  GAAGCCTAACCATCGTGAAGAGTGGCCTCCGCTTTGTGGCCCCAGATGCCTTCCGTTTCACCCCTCGGCT
  CAGTCACCTGAATCTGTCCTCCAATGCGTTGGAGTCCCTCTCCTGGAAAACTGTGCAGGGCCTCTCCCTA
  CAGGACCTGACCCTGTCAGGGAACCCACTGCATTGTTCCTGTGCCCTGTTCTGGCTCCAGCGTTGGGAGC
  AGGAAGGGCTGTGTGGTGTGCATACACAGACGCTTCATGACTCTGGGCCTGGAGACCAGTTCCTCCCACT
  GGGACACAACACCAGTTGTGGTGTACCCACAGTGAAGATCCAGATGCCCAATGACTCTGTGGAAGTGGGC
  GATGACGTGTTTCTGCAGTGCCAGGTGGAGGGGCTGGCCCTACAGCAGGCTGACTGGATCCTCACAGAGC
  TGGAAGGGGCAGCCACCGTGAAGAAATTTGGAGATCTGCCATCCCTGGGGCTGATTCTGGTCAATGTCAC
  CAGTGATCTCAACAAGAAGAATGTGACGTGCTGGGCAGAGAATGATGTGGGCCGGGCCGAGGTCTCTGTC
  CAAGTCAGCGTCTCCTTCCCAGCCAGTGTGCACCTGGGCCTAGCGGTGGAGCAGCATCATTGGTGCATCC
  CCTTCTCGGTGGACGGGCAGCCAGCACCGTCTCTGCGCTGGTTGTTCAACGGCTCTGTGCTCAACGAGAC
  CAGTTTCATCTTCACTCAGTTCTTGGAGTCTGCGCTGACTAATGAGACCATGCGGCACGGCTGCCTGCGC
  CTCAATCAGCCCACGCATGTCAACAACGGGAACTACACCCTGCTGGCGGCCAACCCCTACGGCCAGGCTG
  CCGCCTCCGTCATGGCTGCTTTTATGGACAACCCTTTTGAGTTCAACCCTGAGGACCCCATCCCTGTCTC
  CTTCTCGCCAGTGGACGGTAACAGCACATCAAGAGACCCAGTGGAGAAGAAAGATGAAACCCCTTTTGGG
  GTCTCTGTGGCTGTGGGCCTAGCCGTCTCTGCCGCCCTCTTCCTTTCTGCCCTCCTTCTTGTGCTCAACA
  AATGTGGACAGAGGAGCAAATTTGGGATCAACCGCCCTGCTGTATTAGCTCCAGAGGATGGGCTGGCCAT
  GTCCCTACACTTCATGACACTGGGTGGCAGTTCTCTTTCCCCTACTGAGGGCAAAGGCTCCGGACTCCAG
  GGCCACATCATGGAGAACCCACAGTACTTCAGTGATACCTGTGTCCATCACATCAAGCGCCAGGACATCA
  TTCTCAAGTGGGAGCTAGGGGAGGGAGCCTTTGGAAAGGTCTTTCTCGCTGAGTGCTACAACCTTCTGAA
  TGATCAGGACAAGATGCTCGTGGCTGTCAAGGCACTGAAGGAGGCATCCGAGAATGCTCGGCAGGACTTT
  CAGCGTGAGGCCGAGCTGCTCACCATGCTACAGCACCAGCACATCGTGCGCTTCTTTGGAGTCTGCACCG
  AGGGTGGCCCGTTGCTCATGGTCTTTGAGTACATGCGCCACGGGGACCTCAACCGTTTCCTCCGGTCCCA
  CGGACCTGATGCAAAGCTGCTGGCTGGTGGTGAGGATGTGGCTCCTGGTCCTCTGGGCCTTGGGCAGCTT
  CTGGCTGTGGCTAGCCAGGTGGCTGCTGGTATGGTATATCTAGCCAGCCTGCACTTTGTGCACCGGGATC
  TGGCCACACGCAACTGTCTGGTGGGTCAGGGACTAGTGGTGAAGATTGGAGACTTTGGCATGAGCAGAGA
  CATCTATAGCACAGACTATTACCGAGTGGGAGGTCGGACCATGCTGCCCATCCGCTGGATGCCACCCGAG
  AGCATCCTCTACCGCAAGTTCAGCACCGAGAGCGATGTGTGGAGCTTTGGGGTGGTGCTCTGGGAGATCT
  TCACCTATGGAAAGCAGCCCTGGTACCAGCTCTCCAACACTGAGGCGATCGAGTGTATCACGCAGGGCCG
  GGAGCTGGAGCGGCCGCGCGCCTGCCCTCCTGATGTCTACGCCATCATGCGAGGCTGCTGGCAGCGAGAA
  CCGCAGCAACGCCTCAGCATGAAGGATGTGCACGCGCGTCTGCAGGCCCTGGCACAGGCGCCACCCAGTT
  ACCTGGACGTTCTGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226042 representing NM_001033124
  Red=Cloning site Green=Tags(s)
  
  MLRGQRLGQLGWHRPAAGLGSLMTSLMLACASAASCREVCCPVGPSGLRCTRAGSLDTLRGLRGAGNLTE
  LYVENQQHLQRLEFEDLQGLGELRSLTIVKSGLRFVAPDAFRFTPRLSHLNLSSNALESLSWKTVQGLSL
  QDLTLSGNPLHCSCALFWLQRWEQEGLCGVHTQTLHDSGPGDQFLPLGHNTSCGVPTVKIQMPNDSVEVG
  DDVFLQCQVEGLALQQADWILTELEGAATVKKFGDLPSLGLILVNVTSDLNKKNVTCWAENDVGRAEVSV
  QVSVSFPASVHLGLAVEQHHWCIPFSVDGQPAPSLRWLFNGSVLNETSFIFTQFLESALTNETMRHGCLR
  LNQPTHVNNGNYTLLAANPYGQAAASVMAAFMDNPFEFNPEDPIPVSFSPVDGNSTSRDPVEKKDETPFG
  VSVAVGLAVSAALFLSALLLVLNKCGQRSKFGINRPAVLAPEDGLAMSLHFMTLGGSSLSPTEGKGSGLQ
  GHIMENPQYFSDTCVHHIKRQDIILKWELGEGAFGKVFLAECYNLLNDQDKMLVAVKALKEASENARQDF
  QREAELLTMLQHQHIVRFFGVCTEGGPLLMVFEYMRHGDLNRFLRSHGPDAKLLAGGEDVAPGPLGLGQL
  LAVASQVAAGMVYLASLHFVHRDLATRNCLVGQGLVVKIGDFGMSRDIYSTDYYRVGGRTMLPIRWMPPE
  SILYRKFSTESDVWSFGVVLWEIFTYGKQPWYQLSNTEAIECITQGRELERPRACPPDVYAIMRGCWQRE
  PQQRLSMKDVHARLQALAQAPPSYLDVLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033124
• ORF Size: 2397 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033124.1, NP_001028296.1
• RefSeq Size: 2606 bp
• RefSeq ORF: 2400 bp
• Locus ID: 18211
• UniProt ID: Q3UFB7
• Cytogenetics: 3 38.62 cM
• MW: 88.2 kDa
• Gene Summary: Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.[UniProtKB/Swiss-Prot Function]