Shtn1 (NM_175172) Mouse Tagged ORF Clone

CAT#: MR225987

4930506M07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4930506M07 gene (4930506M07Rik), transcript variant 2

Product Images

Circular map for MR225987

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Shtn1
• Synonyms: 4930506M07Rik; Kiaa1598; mKIAA1598; Shootin1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225987 representing NM_175172
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGCTCGGACGAGGAGAAGCAGCTGCAGCTCATCACCAGCTTGAAGGAGCAAGCCATTGGCGAAT
  ATGAAGACCTTCGAGCAGAGAACCAGAAAACAAAGGAGAAGTGTGACAAAATTAGGCAAGAACGAGATGA
  AGCTGTTAAAAAACTGGAAGAGTTTCAGAAAATTTCACATATGGTTATAGAGGAGGTGAATTTCATGCAG
  AACCATCTTGAAATAGAGAAGACATGTCGAGAGAGCGCTGAAGCCTTGGCGACAAAGCTAAATAAAGAAA
  ATAAAACACTGAAAAGAATCAGCATGCTATACATGGCCAAACTGGGGCCAGACGTAATTACAGAGGAGAT
  AAACATTGATGATGATGACCCAGCCACCGACACAGATGCTGCGGCTGAGACCTGTGTGTCTGTTCAGTGT
  CAGAAGCAAATCAAAGAACTTCGAGATCAAATTGTGTCTGTTCAGGAAGAAAAGAAGGTGTTAGCCATCG
  AGCTGGAAAACCTCAAGAGCAAACTCGGGGAGGTGATGGAGGAGGTAAATAAAGTTAAGCAAGAAAAAGC
  TGTTTTAAATTCAGAAGTCCTTGAGCAGAGGAAAGTCTTAGAAAAATGCAACAGAGTGTCCATGTTGGCT
  GTTGAAGAGTATGAGGAACTGCAAGTGAACCTGGAACTGGAGAAGGACCTTCGCAAGAAAGCAGAGTCTT
  TTGCACAAGAGATGTTCATTGAACAAAACAAACTGAAGAGACAAAGCCACCTTCTGCTGCAGAGCTCCCT
  TCCTGACCAGCAGCTTTTGAAAGCTTTAGACGAAAACGCAAAACTTATCCAGCAGCTTGAAGAAGAGAGG
  ATCCAGCATCAGAAAAAGGTCAAAGAGCTGGAGGAGCGGCTGGAGAATGAAGCACTTCACAAAGAGATCC
  ATAACCTCAGACAACAGCTGGAGCTTCTGGAAGACGACAAGAGGGAGCTAGAGCAGAAATACCAGAGCTC
  GGAGGAGAAGGCCCGGAACCTGAAGCATTCAGTGGATGAACTTCAGAAGCGAGTGAACCAGTCTGAGAAT
  TCGGTACCTCCCCCGCCTCCTCCTCCTCCACCTCTCCCCCCTCCACCTCCCAATCCAATCCGGTCCCTCA
  TGTCTATGATCCGGAAGCGATCTCACCCCAGTGGCAATAGTGCTAAGAAAGAAAAGACAACTCAGCCAGA
  GACAGCTGAGGAAGTCACAGACCTGAAGAGGCAAGCAGTGGAAGAGATGATGGACAGAATTAAGAAGGGA
  GTTCATCTTAGACCGGTTAACCAGACAGCCAGACCCAAGGCAAAGCCAGACTCTCTCAAGGGCTCAGAAA
  GTGCGGTGGATGAGCTGAAGGGAATCCTGGCCTCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225987 representing NM_175172
  Red=Cloning site Green=Tags(s)
  
  MNSSDEEKQLQLITSLKEQAIGEYEDLRAENQKTKEKCDKIRQERDEAVKKLEEFQKISHMVIEEVNFMQ
  NHLEIEKTCRESAEALATKLNKENKTLKRISMLYMAKLGPDVITEEINIDDDDPATDTDAAAETCVSVQC
  QKQIKELRDQIVSVQEEKKVLAIELENLKSKLGEVMEEVNKVKQEKAVLNSEVLEQRKVLEKCNRVSMLA
  VEEYEELQVNLELEKDLRKKAESFAQEMFIEQNKLKRQSHLLLQSSLPDQQLLKALDENAKLIQQLEEER
  IQHQKKVKELEERLENEALHKEIHNLRQQLELLEDDKRELEQKYQSSEEKARNLKHSVDELQKRVNQSEN
  SVPPPPPPPPPLPPPPPNPIRSLMSMIRKRSHPSGNSAKKEKTTQPETAEEVTDLKRQAVEEMMDRIKKG
  VHLRPVNQTARPKAKPDSLKGSESAVDELKGILASQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175172
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175172.4, NP_780381.1
• RefSeq Size: 3767 bp
• RefSeq ORF: 1371 bp
• Locus ID: 71653
• UniProt ID: Q8K2Q9
• Cytogenetics: 19 D2- D3
• MW: 53 kDa
• Gene Summary: Involved in the generation of internal asymmetric signals required for neuronal polarization and neurite outgrowth (PubMed:23864681). Mediates netrin-1-induced F-actin-substrate coupling or 'clutch engagement' within the axon growth cone through activation of CDC42, RAC1 and PAK1-dependent signaling pathway, thereby converting the F-actin retrograde flow into traction forces, concomitantly with filopodium extension and axon outgrowth. Plays a role in cytoskeletal organization by regulating the subcellular localization of phosphoinositide 3-kinase (PI3K) activity at the axonal growth cone. Plays also a role in regenerative neurite outgrowth (By similarity). In the developing cortex, cooperates with KIF20B to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex (PubMed:23864681). Involved in the accumulation of phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the growth cone of primary hippocampal neurons (PubMed:23864681).[UniProtKB/Swiss-Prot Function]