Hace1 (NM_172473) Mouse Tagged ORF Clone

CAT#: MR225877

Hace1 (Myc-DDK-tagged) - Mouse HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1)

Product Images

Circular map for MR225877

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hace1
• Synonyms: 1700042J16Rik; A730034A22Rik; BC025474
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225877 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGAGCGATGGAGCAGCTCAACCGTCTGACGCGCTCGCTGCGCCGCGCGCGCACCGTGGAGCTGC
  CCGAGGATAATGAGACTGCTGTTTACACACTGATGCCAATGGTGATGGCCGACCAGCACAGGTCTGTTTC
  TGAACTCCTATCAAATTCAAAATTTGATGTCAATTATGCATTCGGACGTGTGAAGAGAAGCTTGCTTCAC
  ATTGCTGCAAACTGTGGATCTGTGGAATGCTTGGTTCTTCTGTTGAAGAAAGGCGCAAACCCTAACTATC
  AAGATATTTCAGGCTGTACACCCCTTCATCTCGCTGCTAGAAATGGACAGAAGAAATGCATGAGTAAATT
  ACTGGAGTACAGTGCGGATGTCAACATTTGTAATAATGAAGGCCTTACAGCAATACACTGGCTGGCTGTG
  AATGGGAGGACAGAGCTCCTCCATGACCTCGTGCAACACGTCACAGATGTGGATGTGGAGGATGCCATGG
  GGCAGACAGCGCTACACGTAGCCTGCCAGAACGGGCACAAAACGACAGTGCAGTGCTTGCTAGACAGCGG
  TGCTGATATTAACAGGCCGAATGTATCAGGAGCCACGCCACTGTACTTTGCTTGCAGTCATGGTCAGAGA
  GATACTGCACAGATTCTTCTATTACGAGGAGCCAAATATTTACCAGATAAAAATGGAGTAACCCCTCTAG
  ATCTATGTGTACAGGGTGGTTACGGACAGACTTGTGAAGTGTTAATTCAGTACCACCCGAGGCTTTTCCA
  GACTATTGTTCAAATGACACAGAATGAAGACCTTCGGGAAAACATGTTACGGCAAGTTCTGCAGCATTTG
  TCTCAGCAAAGTGAAAGCCAGTACCTAAAGATCCTGACAGGCCTTGCTGAAGTTGCTACGACAAATGGCC
  ATAAGCTGCTTAGTTTATCTAGCAATTATGATGCGCAGATGAAGAGCCTTTTGAGGATTGTGAGGATATT
  TTGTCATGTCTTTCGAATTGGTCCCTCATCTCCCAGTAATGGAATCGATATGGGCTACAATGGAAATAAA
  ACTCCAAGAAGCCAGGTGTTCAAGCCTTTGGAATTGCTTTGGCACTCATTGGATGAATGGTTAGTTTTAA
  TAGCCACAGAATTAATGAAAAACAAAGAGGATTCAACAGATATTACTTCTATCTTGCTGAAACAAAAAGG
  CCAGGATCAAGAGGCCCCTTCTCTTTCTGCATTTGAGCCACCAGGCCCTGGGAGCTATGAAAGCCTGCCC
  CCTGGTCCAGGGGACTCCAAGCCAGAGGTGCTTGCAGGGGAGCAGGAAGCCAGTGCCGACTGTCAAGACG
  TCATCTCTGTGACAGCCAACCGGCTGAGTGCTGTCATTCAAGCCTTCTACATGTGCTGTTCCTGTCAGAT
  GCCTCCAGGAATGACTTCACCTCGTTTCATTGAGTTTGTCTGCAAGCATGATGAAGTATTGAAATGCTTT
  GTGAATAGAAACCCCAAAATTATATTTGACCATTTTCACTTTCTTCTTGAATGTCCTGAATTGATGTCAA
  GATTCATGCATATCATAAAAGCACAGCCATTTAAAGATCGATGTGAGTGGTTCTATGAACATCTGCACTC
  AGGACAGCCGGACTCAGACATGGTCCACAGACCAGTGAGTGAGAACGATATCCTCCTGGTTCACAGAGAT
  TCTATTTTTAGGAGTAGCTGTGAAATTGTATCAAAAGCAAACTGTGCAAAGCTAAAGCAGGGGATTGCTG
  TACGATTTCATGGAGAAGAAGGCATGGGTCAAGGCGTTGTGCGTGAATGGTTTGATATTCTGTCTAATGA
  GATCGTCAACCCTGACTATGCACTGTTCACCCAGTCAGCAGACGGAACAACTTTCCAGCCCAACAGCAAC
  TCCTATGTCAATCCTGATCACTTGAACTATTTCCGGTTTGCTGGACAGATCTTGGGGTTAGCTTTGAACC
  ACAGGCAGCTGGTCAATATTTACTTCACACGATCATTCTACAAGCACATTCTCGGTATTCCTGTAAATTA
  CCAAGATGTAGCATCTATTGATCCTGAATATGCCAAAAATTTGCAATGGATTTTAGATAATGATATTAGT
  GACTTGGGTCTAGAATTGACTTTTTCTGTTGAGACTGATGTATTTGGAGCTATGGAAGAGGTGCCTTTAA
  AGCCTGGGGGTGGAAGTATTCTTGTGACACAAAATAACAAAGCGGAGTATGTCCAGCTCGTTACTGAACT
  GCGTATGACAAGAGCCATTCAGCCGCAGATCAATGCGTTTTTACAGGGTTTTCATATGTTCATCCCGCCT
  TCCCTCATACAACTGTTTGACGAGTATGAATTGGAGCTACTGCTTTCTGGCATGCCGGAAATTGATGTAA
  ATGATTGGATAAAAAATACAGAATACACAAGTGGCTATGAAAGAGAAGATCCAGTTATTCAGTGGTTCTG
  GGAAGTTGTGGAAGACATTACTCAAGAAGAAAGAGTTCTTCTCTTGCAGTTCGTTACTGGCAGTTCCAGG
  GTCCCGCATGGTGGCTTTGCCAATATTATGGGTGGAAGTGGTTTGCAAAACTTTACAATTGCTGCTGTGC
  CATATACTCCAAATCTTTTACCAACTTCCAGCACATGCATCAACATGCTCAAGTTACCTGAATACCCAAG
  TAAAGAAATACTCAAGGACAGACTCCTTGTAGCACTGCATTGTGGCAGCTATGGTTATACAATGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225877 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLH
  IAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAV
  NGRTELLHDLVQHVTDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQR
  DTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGQTCEVLIQYHPRLFQTIVQMTQNEDLRENMLRQVLQHL
  SQQSESQYLKILTGLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNK
  TPRSQVFKPLELLWHSLDEWLVLIATELMKNKEDSTDITSILLKQKGQDQEAPSLSAFEPPGPGSYESLP
  PGPGDSKPEVLAGEQEASADCQDVISVTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCF
  VNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVSENDILLVHRD
  SIFRSSCEIVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSN
  SYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDIS
  DLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPP
  SLIQLFDEYELELLLSGMPEIDVNDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSR
  VPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172473
• ORF Size: 2730 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172473.3, NP_766061.2
• RefSeq Size: 3785 bp
• RefSeq ORF: 2730 bp
• Locus ID: 209462
• UniProt ID: Q3U0D9
• Cytogenetics: 10 B2
• MW: 102.1 kDa
• Gene Summary: E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens (By similarity). May also act as a transcription regulator via its interaction with RARB.[UniProtKB/Swiss-Prot Function]