Pik3cd (NM_001029837) Mouse Tagged ORF Clone

CAT#: MR225831

Pik3cd (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase catalytic delta polypeptide (Pik3cd), transcript variant 2

Product Images

Circular map for MR225831

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pik3cd
• Synonyms: 2410099E07Rik; 2610208K16Rik; AW545373; p110delta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225831 representing NM_001029837
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCCTGGGGTGGACTGCCCCATGGAGTTCTGGACCAAAGAGGAGAGCCAGAGCGTGGTTGTTGACT
  TCTTGCTGCCCACAGGGGTCTACTTGAACTTCCCCGTGTCCCGCAATGCCAACCTCAGCACCATCAAGCA
  GGTGCTGTGGCACCGTGCACAGTATGAGCCACTCTTCCACATGCTCAGTGACCCCGAGGCCTATGTGTTC
  ACCTGTGTGAACCAGACGGCGGAGCAGCAGGAGTTGGAGGATGAGCAGCGGAGGCTGTGCGACATCCAGC
  CCTTCCTGCCCGTGCTGCGCCTCGTGGCCCGAGAGGGGGACCGCGTGAAGAAGCTCATTAACTCCCAGAT
  CAGCCTCCTCATTGGCAAAGGTCTCCATGAGTTTGATTCCCTGCGGGACCCGGAAGTAAACGACTTCCGC
  ACTAAGATGCGCCAGTTTTGTGAAGAGGCTGCTGCTCACCGCCAGCAGCTGGGCTGGGTGGAATGGCTGC
  AGTACAGCTTCCCCCTGCAGCTGGAGCCCTCAGCAAGGGGTTGGCGGGCCGGCTTATTGCGTGTCAGCAA
  CCGAGCCCTGCTGGTCAACGTGAAGTTCGAGGGCAGTGAGGAGAGCTTCACCTTCCAGGTATCCACCAAG
  GACATGCCCCTGGCACTGATGGCCTGTGCCCTCCGAAAAAAGGCCACAGTGTTCCGGCAGCCTCTGGTGG
  AGCAGCCTGAGGAATATGCCCTGCAGGTGAACGGGAGGCACGAATACCTCTACGGCAACTACCCGCTCTG
  CCACTTTCAGTACATCTGCAGCTGCCTACACAGCGGGCTGACCCCTCATCTGACCATGGTCCACTCCTCC
  TCCATCCTTGCTATGCGGGATGAGCAGAGCAATCCTGCCCCCCAAGTACAGAAACCACGTGCCAAACCTC
  CCCCGATCCCTGCCAAGAAGCCCTCCTCTGTGTCCCTGTGGTCCCTGGAACAGCCATTCTCCATTGAGCT
  GATCGAGGGCCGAAAAGTGAATGCTGACGAGCGGATGAAGCTGGTTGTTCAGGCCGGGCTCTTCCATGGC
  AATGAGATGCTGTGCAAGACTGTGTCAAGCTCGGAGGTGAATGTATGCTCAGAGCCCGTGTGGAAGCAGC
  GACTGGAGTTCGATATCAGCGTCTGTGACCTCCCGCGCATGGCTCGACTCTGTTTTGCTCTCTATGCCGT
  CGTGGAGAAGGCTAAGAAGGCACGCTCCACAAAGAAGAAGTCTAAGAAGGCGGACTGCCCCATCGCTTGG
  GCCAACCTCATGCTATTCGACTACAAAGATCAGCTCAAGACGGGGGAGCGCTGCCTCTACATGTGGCCCT
  CTGTCCCAGATGAGAAGGGAGAGCTGCTGAATCCTGCGGGTACAGTGCGCGGGAACCCCAACACGGAGAG
  TGCCGCTGCCCTGGTCATCTACCTGCCTGAGGTGGCCCCCCACCCTGTGTACTTCCCCGCTCTGGAGAAG
  ATCCTGGAGCTGGGGCGTCACGGGGAGCGTGGGCGCATCACGGAGGAGGAGCAGCTGCAGCTGCGGGAGA
  TCCTGGAACGGCGGGGATCCGGGGAACTGTACGAACATGAGAAGGACCTGGTGTGGAAGATGCGCCACGA
  AGTCCAGGAGCATTTCCCAGAGGCGCTGGCCCGCCTGCTGCTGGTCACCAAGTGGAATAAACACGAGGAT
  GTGGCCCAGCTGTCCCAGATGCTCTATTTGCTGTGCTCCTGGCCCGAGCTGCCTGTGCTGAGCGCCCTGG
  AACTTCTGGACTTTAGCTTTCCCGACTGCTACGTGGGCTCCTTCGCCATCAAGTCCCTTCGGAAGCTGAC
  GGACGATGAGCTCTTCCAGTACCTTCTGCAGCTGGTGCAAGTGCTCAAATATGAGTCCTACCTGGACTGC
  GAGCTGACCAAATTCTTGCTGGGCCGAGCCCTGGCTAACCGCAAGATCGGACACTTCCTGTTCTGGCACC
  TCCGCTCTGAGATGCACGTACCATCAGTGGCTCTGCGGTTTGGTCTCATCATGGAAGCCTACTGCAGAGG
  CAGCACCCACCACATGAAGGTGCTGATGAAGCAGGGGGAAGCACTGAGCAAGCTTAAGGCACTGAATGAC
  TTTGTGAAGGTGAGTTCCCAGAAGACCACCAAGCCCCAAACCAAGGAGATGATGCATATGTGCATGCGCC
  AGGAGACCTACATGGAGGCCCTGTCCCACCTGCAGTCTCCACTCGACCCCAGCACCCTGCTGGAGGAAGT
  CTGTGTGGAGCAGTGCACCTTCATGGACTCCAAAATGAAGCCCCTGTGGATCATGTACAGCAGCGAGGAG
  GCGGGCAGTGCTGGCAACGTGGGCATCATCTTTAAGAACGGGGATGACCTCCGCCAGGACATGCTGACTC
  TGCAGATGATCCAGCTCATGGACGTCCTGTGGAAGCAGGAGGGCCTGGACCTGAGGATGACGCCCTACGG
  CTGCCTCCCCACCGGGGACCGCACAGGTCTCATCGAGGTGGTCCTCCACTCGGACACCATCGCCAACATC
  CAGCTGAACAAAAGCAACATGGCGGCCACAGCTGCCTTCAACAAGGACGCCCTGCTCAACTGGCTCAAGT
  CCAAGAACCCTGGGGAGGCCCTGGATCGGGCCATTGAGGAATTCACCCTCTCCTGTGCTGGCTACTGTGT
  GGCCACATATGTTCTGGGCATCGGTGACCGGCACAGCGACAACATCATGATCAGAGAGAGTGGGCAGCTC
  TTCCACATTGATTTTGGCCACTTTCTGGGGAACTTCAAGACCAAGTTTGGAATCAACCGAGAGCGCGTCC
  CCTTCATTCTCACCTACGACTTTGTCCACGTGATCCAGCAGGGGAAGACTAACAACAGTGAGAAGTTTGA
  AAGGTTCCGCGGCTACTGTGAACGAGCCTATACCATCCTGCGGCGCCACGGGCTGCTTTTCCTCCATCTC
  TTCGCCCTGATGCGGGCCGCAGGTCTGCCTGAGCTTAGCTGCTCCAAAGATATCCAGTATCTCAAGGACT
  CTCTGGCACTGGGGAAGACGGAGGAAGAGGCGCTAAAGCACTTCCGGGTGAAGTTCAACGAAGCTCTCCG
  AGAAAGCTGGAAAACCAAAGTCAACTGGCTGGCGCACAATGTGTCCAAGGATAACCGACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225831 representing NM_001029837
  Red=Cloning site Green=Tags(s)
  
  MPPGVDCPMEFWTKEESQSVVVDFLLPTGVYLNFPVSRNANLSTIKQVLWHRAQYEPLFHMLSDPEAYVF
  TCVNQTAEQQELEDEQRRLCDIQPFLPVLRLVAREGDRVKKLINSQISLLIGKGLHEFDSLRDPEVNDFR
  TKMRQFCEEAAAHRQQLGWVEWLQYSFPLQLEPSARGWRAGLLRVSNRALLVNVKFEGSEESFTFQVSTK
  DMPLALMACALRKKATVFRQPLVEQPEEYALQVNGRHEYLYGNYPLCHFQYICSCLHSGLTPHLTMVHSS
  SILAMRDEQSNPAPQVQKPRAKPPPIPAKKPSSVSLWSLEQPFSIELIEGRKVNADERMKLVVQAGLFHG
  NEMLCKTVSSSEVNVCSEPVWKQRLEFDISVCDLPRMARLCFALYAVVEKAKKARSTKKKSKKADCPIAW
  ANLMLFDYKDQLKTGERCLYMWPSVPDEKGELLNPAGTVRGNPNTESAAALVIYLPEVAPHPVYFPALEK
  ILELGRHGERGRITEEEQLQLREILERRGSGELYEHEKDLVWKMRHEVQEHFPEALARLLLVTKWNKHED
  VAQLSQMLYLLCSWPELPVLSALELLDFSFPDCYVGSFAIKSLRKLTDDELFQYLLQLVQVLKYESYLDC
  ELTKFLLGRALANRKIGHFLFWHLRSEMHVPSVALRFGLIMEAYCRGSTHHMKVLMKQGEALSKLKALND
  FVKVSSQKTTKPQTKEMMHMCMRQETYMEALSHLQSPLDPSTLLEEVCVEQCTFMDSKMKPLWIMYSSEE
  AGSAGNVGIIFKNGDDLRQDMLTLQMIQLMDVLWKQEGLDLRMTPYGCLPTGDRTGLIEVVLHSDTIANI
  QLNKSNMAATAAFNKDALLNWLKSKNPGEALDRAIEEFTLSCAGYCVATYVLGIGDRHSDNIMIRESGQL
  FHIDFGHFLGNFKTKFGINRERVPFILTYDFVHVIQQGKTNNSEKFERFRGYCERAYTILRRHGLLFLHL
  FALMRAAGLPELSCSKDIQYLKDSLALGKTEEEALKHFRVKFNEALRESWKTKVNWLAHNVSKDNRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001029837
• ORF Size: 3141 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001029837.2, NP_001025008.2
• RefSeq Size: 4909 bp
• RefSeq ORF: 3144 bp
• Locus ID: 18707
• Cytogenetics: 4 E2
• MW: 120.6 kDa
• Gene Summary: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Mediates immune responses. Plays a role in B-cell development, proliferation, migration, and function. Required for B-cell receptor (BCR) signaling. Mediates B-cell proliferation response to anti-IgM, anti-CD40 and IL4 stimulation. Promotes cytokine production in response to TLR4 and TLR9. Required for antibody class switch mediated by TLR9. Involved in the antigen presentation function of B-cells. Involved in B-cell chemotaxis in response to CXCL13 and sphingosine 1-phosphate (S1P). Required for proliferation, signaling and cytokine production of naive, effector and memory T-cells. Required for T-cell receptor (TCR) signaling. Mediates TCR signaling events at the immune synapse. Activation by TCR leads to antigen-dependent memory T-cell migration and retention to antigenic tissues. Together with PIK3CG participates in T-cell development. Contributes to T-helper cell expansion and differentiation. Required for T-cell migration mediated by homing receptors SELL/CD62L, CCR7 and S1PR1 and antigen dependent recruitment of T-cells. Together with PIK3CG is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in NK cell receptor activation. Have a role in NK cell maturation and cytokine production. Together with PIK3CG is involved in neutrophil chemotaxis and extravasation. Together with PIK3CG participates in neutrophil respiratory burst. Have important roles in mast-cell development and mast cell mediated allergic response. Involved in stem cell factor (SCF)-mediated proliferation, adhesion and migration. Required for allergen-IgE-induced degranulation and cytokine release. The lipid kinase activity is required for its biological function.[UniProtKB/Swiss-Prot Function]