Numb (NM_001136075) Mouse Tagged ORF Clone

CAT#: MR225820

Numb (Myc-DDK-tagged) - Mouse numb gene homolog (Drosophila) (Numb), transcript variant 1

Product Images

Circular map for MR225820

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Numb
• Synonyms: m-num; Nb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225820 representing NM_001136075
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAACTACGGCAAAGCTTCAGGAGAAAGAAAGACGTTTATGTCCCAGAGGCCAGCCGTCCACATC
  AGTGGCAGACAGATGAAGAAGGAGTCCGCACTGGAAAGTGTAGCTTCCCAGTTAAGTACCTCGGCCACGT
  AGAAGTTGATGAGTCAAGAGGAATGCACATCTGTGAAGATGCCGTAAAGAGATTGAAAGCTGAAAGGAAG
  TTCTTCAAAGGCTTCTTTGGAAAAACGGGAAAGAAAGCAGTGAAGGCCGTTCTGTGGGTGTCAGCGGATG
  GGCTCAGAGTTGTGGACGAGAAAACTAAGGACCTCATAGTTGACCAGACAATAGAAAAAGTTTCCTTCTG
  TGCCCCAGATAGGAACTTTGACAGAGCCTTTTCTTACATATGTCGTGATGGCACCACTCGGCGATGGATC
  TGTCATTGTTTCATGGCTGTCAAAGACACGGGGGAAAGACTGAGCCACGCCGTGGGCTGTGCTTTTGCAG
  CCTGTTTAGAGCGTAAACAGAAGCGGGAGAAGGAGTGTGGCGTCACTGCTACTTTTGATGCCAGTAGAAC
  CACTTTCACAAGAGAAGGATCATTCCGTGTCACAACTGCCACTGAGCAAGCCGAAAGAGAGGAGATCATG
  AAACAGTTGCAAGATGCCAAGAAAGCTGAGACAGACAAGACAGTTGTTGGTCCATCAGTGGCTCCTGGCA
  ACACTGCTCCATCCCCATCCTCTCCCACCTCTCCCACTCCGGATGGCACTGCATCTTCAGAGATGAACAA
  TCCCCATGCTATCCCACGCCGGCATGCACCAATTGAACAGCTTGCTCGTCAAGGCTCTTTCCGGGGATTT
  CCTGCTCTTAGCCAGAAGATGTCACCCTTTAAACGCCAGCTGTCCCTACGCATCAATGAGTTGCCTTCCA
  CTATGCAGAGGAAGACCGATTTCCCAATAAAAAACACAGTGCCCGAGGTGGAAGGAGAGGCCGAGAGCAT
  CAGCTCCTTGTGTTCCCAGATCACCAGTGCCTTCAGCACGCCCTCTGAGGACCCCTTCTCCTCCGCCCCA
  ATGACCAAACCAGTGACATTGGTGGCACCACAGTCTCCTGTGTTACAAGCTAATGGCACTGACTCAGCCT
  CCCATGTGCTTACTGCTAAGCCAGCCAATACTGCTCTAGCACACGTAGCAATGCCTGTCCGTGAAACCAA
  CCCCTGGGCCCATGTCCCTGATGCTGCTAACAAGGAAATTGCAGCCATACATCCGGGGACTGAGTGGGGT
  CAGTCTTCTGGTGCTGCCTCTCCAGGTCTCTTCCAGGCTGGTCACAGACGCACTCCCTCTGAAGCTGACC
  GCTGGTTAGAAGAAGTGTCAAAGAGTGTGCGGGCCCAGCAGCCTCAGGTCTCAGCTGCCCCTCTGCAGCC
  AGTTCTCCAGCCGCCTCCGCCCGCCGCCATTGCCCCTCCAGCACCTCCTTTCCAAGGACATGCCTTCCTC
  ACGTCCCAGCCTGTGCCCGTGGGTGTGGTCCCACCCCTACAACCAGCCTTTGTCCCTACCCAGTCCTACC
  CTGTGGCCAACGGGATGCCCTACCCAGCCTCTAATGTGCCTGTAGTGGGCATCACCCCATCCCAGATGGT
  AGCCAATGTGTTTGGCACTGCAGGCCACCCTCAGACAACTCATCCACATCAGTCGCCAAGCCTGGCCAAG
  CAGCAGACATTCCCTCAATATGAGACAAGTAGTGCTACCACCAGTCCCTTCTTTAAGCCTCCTGCTCAGC
  ACCTCAATGGTTCTGCAGCTTTCAATGGTGTAGACAATGGTGGGCTAGCCTCAGGAAACAGGCATGCAGA
  AGTCCCTCCAGGCACCTGCCCAGTGGATCCTTTCGAAGCTCAGTGGGCCGCACTAGAAAGCAAGTCCAAG
  CAGCGTACAAATCCTTCTCCTACCAACCCTTTCTCCAGTGACTTACAGAAAACATTTGAAATAGAACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225820 representing NM_001136075
  Red=Cloning site Green=Tags(s)
  
  MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYLGHVEVDESRGMHICEDAVKRLKAERK
  FFKGFFGKTGKKAVKAVLWVSADGLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRRWI
  CHCFMAVKDTGERLSHAVGCAFAACLERKQKREKECGVTATFDASRTTFTREGSFRVTTATEQAEREEIM
  KQLQDAKKAETDKTVVGPSVAPGNTAPSPSSPTSPTPDGTASSEMNNPHAIPRRHAPIEQLARQGSFRGF
  PALSQKMSPFKRQLSLRINELPSTMQRKTDFPIKNTVPEVEGEAESISSLCSQITSAFSTPSEDPFSSAP
  MTKPVTLVAPQSPVLQANGTDSASHVLTAKPANTALAHVAMPVRETNPWAHVPDAANKEIAAIHPGTEWG
  QSSGAASPGLFQAGHRRTPSEADRWLEEVSKSVRAQQPQVSAAPLQPVLQPPPPAAIAPPAPPFQGHAFL
  TSQPVPVGVVPPLQPAFVPTQSYPVANGMPYPASNVPVVGITPSQMVANVFGTAGHPQTTHPHQSPSLAK
  QQTFPQYETSSATTSPFFKPPAQHLNGSAAFNGVDNGGLASGNRHAEVPPGTCPVDPFEAQWAALESKSK
  QRTNPSPTNPFSSDLQKTFEIEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136075
• ORF Size: 1959 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136075.2, NP_001129547.1
• RefSeq Size: 1962 bp
• RefSeq ORF: 1962 bp
• Locus ID: 18222
• UniProt ID: Q9QZS3
• Cytogenetics: 12 D1
• MW: 71.3 kDa
• Gene Summary: This gene encodes a conserved protein that is distributed asymmetrically during cell division in the developing embryo. The encoded protein participates in cell fate decisions by interacting with the Notch receptor. Loss of function of this gene results in severe defects in neural development and loss of viability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]