Dgat1 (NM_010046) Mouse Tagged ORF Clone

CAT#: MR225809

Dgat1 (Myc-DDK-tagged) - Mouse diacylglycerol O-acyltransferase 1 (Dgat1)

Product Images

Circular map for MR225809

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dgat1
• Synonyms: ARAT; C75990; D15Ertd23e; Dgat
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225809 representing NM_010046
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACCGCGGAGGCGCGGGAAGCTCTCGGCGTCGGAGGACCGGCTCGCGGGTTTCCGTCCAGGGTG
  GTAGTGGGCCCAAGGTAGAAGAGGACGAGGTGCGAGACGCGGCTGTGAGCCCCGACTTGGGCGCCGGGGG
  TGACGCGCCGGCTCCGGCTCCGGCTCCAGCCCATACCCGGGACAAAGACGGGCGGACCAGCGTGGGCGAC
  GGCTACTGGGATCTGAGGTGCCATCGTCTGCAAGATTCTTTGTTCAGCTCAGACAGTGGTTTCAGCAATT
  ATCGTGGTATCCTGAATTGGTGTGTGGTGATGCTGATCCTGAGTAATGCAAGGTTATTTTTAGAGAACCT
  TATCAAGTATGGCATCCTGGTGGATCCTATCCAGGTGGTGTCTCTGTTTCTGAAGGACCCCTACAGCTGG
  CCTGCCCCATGCGTGATTATTGCATCCAATATTTTTGTTGTGGCTGCATTTCAGATTGAGAAGCGCCTGG
  CAGTGGGTGCCCTGACAGAGCAGATGGGGCTGCTGCTACATGTGGTTAACCTGGCCACAATCATCTGCTT
  CCCAGCAGCTGTGGCCTTACTGGTTGAGTCTATCACTCCAGTGGGTTCCGTGTTTGCTCTGGCATCATAC
  TCCATCATGTTCCTCAAGCTTTATTCCTACCGGGATGTCAACCTGTGGTGCCGCCAGCGAAGGGTCAAGG
  CCAAAGCTGTCTCTACAGGGAAGAAGGTCAGTGGGGCTGCTGCCCAGCAAGCTGTGAGCTATCCAGACAA
  CCTGACCTACCGAGATCTCTATTACTTCATCTTTGCTCCTACTTTGTGTTATGAACTCAACTTTCCTCGG
  TCCCCCCGAATACGAAAGCGCTTTCTGCTACGACGAGTTCTTGAGATGCTCTTTTTTACCCAGCTTCAAG
  TGGGGCTGATCCAACAGTGGATGGTCCCTACTATCCAGAACTCCATGAAGCCCTTCAAGGATATGGACTA
  TTCACGGATCATTGAGCGTCTCTTAAAGCTGGCGGTCCCCAACCATCTGATCTGGCTTATCTTCTTCTAT
  TGGTTTTTCCACTCCTGTCTCAATGCTGTGGCAGAGCTTCTGCAGTTTGGAGACCGCGAGTTCTACAGAG
  ATTGGTGGAATGCTGAGTCTGTCACCTACTTTTGGCAGAACTGGAATATCCCCGTGCACAAGTGGTGCAT
  CAGACACTTCTACAAGCCTATGCTCAGACATGGCAGCAGCAAATGGGTGGCCAGGACAGGAGTATTTTTG
  ACCTCAGCCTTCTTCCATGAGTACCTAGTGAGCGTTCCCCTGCGGATGTTCCGCCTCTGGGCATTCACAG
  CCATGATGGCTCAGGTCCCACTGGCCTGGATTGTGGGCCGATTCTTCCAAGGGAACTATGGCAATGCAGC
  TGTGTGGGTGACACTCATCATTGGGCAACCGGTGGCTGTGCTCATGTATGTCCACGACTACTACGTGCTC
  AACTACGATGCCCCAGTGGGGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225809 representing NM_010046
  Red=Cloning site Green=Tags(s)
  
  MGDRGGAGSSRRRRTGSRVSVQGGSGPKVEEDEVRDAAVSPDLGAGGDAPAPAPAPAHTRDKDGRTSVGD
  GYWDLRCHRLQDSLFSSDSGFSNYRGILNWCVVMLILSNARLFLENLIKYGILVDPIQVVSLFLKDPYSW
  PAPCVIIASNIFVVAAFQIEKRLAVGALTEQMGLLLHVVNLATIICFPAAVALLVESITPVGSVFALASY
  SIMFLKLYSYRDVNLWCRQRRVKAKAVSTGKKVSGAAAQQAVSYPDNLTYRDLYYFIFAPTLCYELNFPR
  SPRIRKRFLLRRVLEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIWLIFFY
  WFFHSCLNAVAELLQFGDREFYRDWWNAESVTYFWQNWNIPVHKWCIRHFYKPMLRHGSSKWVARTGVFL
  TSAFFHEYLVSVPLRMFRLWAFTAMMAQVPLAWIVGRFFQGNYGNAAVWVTLIIGQPVAVLMYVHDYYVL
  NYDAPVGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010046
• ORF Size: 1494 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010046.3
• RefSeq Size: 1776 bp
• RefSeq ORF: 1497 bp
• Locus ID: 13350
• UniProt ID: Q9Z2A7
• Cytogenetics: 15 35.99 cM
• MW: 57.2 kDa
• Gene Summary: Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.[UniProtKB/Swiss-Prot Function]