Pard6a (NM_001047436) Mouse Tagged ORF Clone

CAT#: MR225774

Pard6a (Myc-DDK-tagged) - Mouse par-6 (partitioning defective 6,) homolog alpha (C. elegans) (Pard6a), transcript variant 3

Product Images

Circular map for MR225774

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pard6a
• Synonyms: 0710008C04Rik; 2610010A15Rik; Par-6; PAR-6A; Par6; PAR6alpha; Par6c; TAX40; Tip-40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225774 representing NM_001047436
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGCGATCCAGTTTGACGCCGAGTTCCGACGCTTTGCACTACCCCGCACTTCGGTGAGAGGCTTTC
  AGGAGTTCTCGCGATTGCTGTGTGTGGTACACCAGATCCCTGGCCTGGACGTCCTGCTTGGCTATACGGA
  TGCTCACGGTGACTTGCTGCCCCTCACCAACGATGACAGTTTGCACCGGGCCCTGGCCAGCGGGCCCCCA
  CCTCTGCGCCTCTTGGTTCAGAAACGGGCAGAAGGTGACTCGAGTGGCCTGGCTTTTGCCTCCAACTCTC
  TACAAAGGCGCAAGAAAGGGCTCCTGCTACGACCAGTGGCACCTCTGCGCACCAGGCCACCCTTGTTAAT
  CAGCTTGCCCCAAGATTTCCGCCAGGTGTCTTCAGTTATAGATGTGGACCTACTACCTGAGACCCACCGA
  CGAGTGAGGCTGCACAAACATGGTTCAGACCGTCCCCTGGGCTTCTACATTCGAGATGGCATGAGTGTTC
  GCGTGGCTCCCCAGGGCCTGGAGCGGGTTCCAGGTATCTTCATCTCCCGCCTGGTACGTGGGGGCCTGGC
  TGAGAGTACAGGGCTGCTGGCGGTCAGTGATGAGATCCTTGAGGTCAACGGCATTGAGGTGGCCGGGAAG
  ACCTTGGACCAAGTGACGGACATGATGGTCGCCAACAGCCACAACCTCATCGTCACTGTCAAGCCTGCCA
  ACCAGCGTAATAATGTGGTACGGGGGGCATCTGGGCGTCTGACAGGGCCTTCCTCTGTAGGGCCTGGGCC
  TACTGATCCTGACAGTGACGATGACAGCAGTGACCTGGTCATTGAGAATCGCCACCCTCCCTGTTCTAAT
  GGGCTGTCTCAGGGGCCCCTGTGCTGGGACCTGCAACCTGGCTGCCTACTTCCTGGTGCTGGCAGCTCTC
  TGCCCTCCTTGGATAGCAGAGAGCAAGCCAATTCTGGCTGGGGGAATGGCATGCGAGGTGATGTTAGCGG
  ATTCAGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225774 representing NM_001047436
  Red=Cloning site Green=Tags(s)
  
  MRAIQFDAEFRRFALPRTSVRGFQEFSRLLCVVHQIPGLDVLLGYTDAHGDLLPLTNDDSLHRALASGPP
  PLRLLVQKRAEGDSSGLAFASNSLQRRKKGLLLRPVAPLRTRPPLLISLPQDFRQVSSVIDVDLLPETHR
  RVRLHKHGSDRPLGFYIRDGMSVRVAPQGLERVPGIFISRLVRGGLAESTGLLAVSDEILEVNGIEVAGK
  TLDQVTDMMVANSHNLIVTVKPANQRNNVVRGASGRLTGPSSVGPGPTDPDSDDDSSDLVIENRHPPCSN
  GLSQGPLCWDLQPGCLLPGAGSSLPSLDSREQANSGWGNGMRGDVSGFSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001047436
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001047436.2, NP_001040901.1
• RefSeq Size: 1291 bp
• RefSeq ORF: 993 bp
• Locus ID: 56513
• Cytogenetics: 8 D3
• MW: 36 kDa
• Gene Summary: Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (PubMed:15761148). Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization (By similarity).[UniProtKB/Swiss-Prot Function]