Prom1 (NM_001163582) Mouse Tagged ORF Clone

CAT#: MR225620

Prom1 (Myc-DDK-tagged) - Mouse prominin 1 (Prom1), transcript variant 5

Product Images

Circular map for MR225620

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prom1
• Synonyms: 4932416E19Rik; AC133; CD133; Prom; Prom-1; Proml1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225620 representing NM_001163582
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTCGTCTTCAGTGCCCTGCTGTTACTGGGGCTGTGTGGAAAGATCTCTTCAGAAGGTCAGCCTG
  CATTCCATAACACTCCTGGGGCTATGAATTATGAATTGCCTACCACCAAATATGAGACCCAAGATACCTT
  CAATGCTGGGATTGTTGGCCCTCTCTACAAAATGGTGCACATCTTCCTCAACGTGGTCCAGCCGAATGAC
  TTCCCTCTAGATTTGATCAAAAAACTCATACAGAACAAGAACTTTGACATCTCAGTTGATTCCAAGGAGA
  TTGCCCTCTATGAGATCGGAGTCCTTATCTGCGCCATCCTGGGACTGCTGTTCATTATCCTCATGCCTCT
  GGTGGGCTGCTTCTTTTGTATGTGCCGTTGCTGCAACAAATGCGGCGGAGAGATGCACCAGCGGCAGAAG
  CAGAATGCGCCATGCAGGAGGAAGTGCTTGGGCCTCTCCCTCCTGGTGATTTGTCTGCTCATGAGCCTTG
  GCATTATATATGGCTTTGTGGCTAACCAGCAGACCAGGACTCGGATCAAAGGGACCCAGAAACTGGCAAA
  GAGCAATTTCAGAGACTTTCAAACACTCCTGACTGAAACACCAAAGCAAATTGACTATGTAGTGGAGCAG
  TACACCAACACCAAGAACAAGGCATTCTCAGACCTGGATGGCATCGGCTCCGTGCTGGGAGGCAGAATAA
  AGGACCAACTAAAACCCAAAGTAACTCCTGTCCTCGAAGAGATTAAGGCCATGGCGACAGCCATCAAACA
  GACCAAGGATGCCCTGCAGAACATGAGCAGCAGCCTGAAAAGTCTCCAAGATGCAGCCACCCAGCTCAAT
  ACCAACCTGAGCTCTGTGAGAAACAGCATCGAGAATTCGCTCAGCAGCAGTGACTGTACCTCAGATCCAG
  CCAGCAAGATCTGCGATAGCATCAGACCAAGCCTAAGCAGTCTGGGGAGCAGCCTCAATTCAAGTCAGGG
  GTATACGACAATTGATGAAATACCCAATACAATACAAAACCAAACTGTGGATGTCATCAAAGACGTCAAA
  AATACCTTGGACTCCATTAGCTCCAACATTAAGGACATGAGCCAAAGTATTCCTATTGAGGATATGCTGT
  TACAGGTCTCCCATTACCTTAATAACAGCAACAGATACTTAAACCAGGAGCTGCCCAAGCTGGAAGAATA
  TGACTCGTACTGGTGGCTGGGTGGCTTGATTGTCTGCTTTCTGCTGACTCTCATTGTGACCTTCTTTTTC
  CTGGGCTTGCTGTGTGGTGTGTTTGGCTATGACAAGCATGCCACCCCAACTAGAAGAGGCTGTGTGTCCA
  ACACTGGAGGCATCTTCCTCATGGCTGGGGTTGGATTCGGCTTCCTTTTTTGCTGGATATTGATGATCCT
  TGTGGTTCTTACGTTTGTTGTTGGTGCAAATGTGGAAAAGTTGCTCTGCGAACCTTATGAAAACAAGAAA
  TTATTACAGGTTTTGGACACTCCCTATCTGCTCAAGGAACAATGGCAATTTTATCTTTCTGGCATGCTAT
  TCAATAACCCAGACATTAACATGACCTTTGAGCAAGTCTACAGGGATTGCAAAAGAGGTCGAGGTATATA
  TGCTGCTTTTCAGCTTGAGAATGTCGTCAACGTCAGTGATCATTTCAACATTGACCAGATTTCTGAAAAC
  ATAAATACGGAGTTGGAAAACCTGAATGTGAACATTGATAGCATTGAACTGTTGGATAACACAGGAAGGA
  AGAGCCTCGAGGACTTTGCACATTCTGGGATAGATACAATCGATTATTCCACATACTTGAAGGAGACTGA
  GAAATCCCCTACTGAAGTGAATCTGCTGACATTTGCCTCTACCCTGGAAGCAAAAGCAAACCAGTTGCCT
  GAAGGAAAGCTGAAACAGGCCTTCTTACTGGATGTACAGAATATAAGAGCCATCCACCAGCATCTCCTCC
  CTCCTGTGCAGCAATCACTGAATACGTTAAGACAAAGTGTCTGGACCCTCCAGCAAACAAGCAACAAGTT
  GCCGGAGAAAGTGAAGAAGATCCTTGCCTCTTTGGACTCTGTTCAGCATTTCCTCACCAATAACGTTTCC
  CTCATCGTTATCGGGGAAACGAAGAAGTTTGGGAAAACAATACTAGGCTACTTTGAACATTATCTGCACT
  GGGTCTTTTATGCCATCACAGAGAAGATGACATCCTGCAAACCCATGGCCACCGCGATGGACTCTGCTGT
  TAATGGCATTCTGTGTGGCTATGTTGCGGACCCTCTGAATTTGTTCTGGTTCGGCATAGGGAAAGCCACG
  GTGCTCTTACTTCCGGCTGTAATCATTGCTATCAAGCTGGCCAAGTACTATCGCAGGATGGATTCAGAGG
  ATGTATACGACGACTCGTCCGTCTCGGGGATGTGGCATTTCACTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225620 representing NM_001163582
  Red=Cloning site Green=Tags(s)
  
  MALVFSALLLLGLCGKISSEGQPAFHNTPGAMNYELPTTKYETQDTFNAGIVGPLYKMVHIFLNVVQPND
  FPLDLIKKLIQNKNFDISVDSKEIALYEIGVLICAILGLLFIILMPLVGCFFCMCRCCNKCGGEMHQRQK
  QNAPCRRKCLGLSLLVICLLMSLGIIYGFVANQQTRTRIKGTQKLAKSNFRDFQTLLTETPKQIDYVVEQ
  YTNTKNKAFSDLDGIGSVLGGRIKDQLKPKVTPVLEEIKAMATAIKQTKDALQNMSSSLKSLQDAATQLN
  TNLSSVRNSIENSLSSSDCTSDPASKICDSIRPSLSSLGSSLNSSQGYTTIDEIPNTIQNQTVDVIKDVK
  NTLDSISSNIKDMSQSIPIEDMLLQVSHYLNNSNRYLNQELPKLEEYDSYWWLGGLIVCFLLTLIVTFFF
  LGLLCGVFGYDKHATPTRRGCVSNTGGIFLMAGVGFGFLFCWILMILVVLTFVVGANVEKLLCEPYENKK
  LLQVLDTPYLLKEQWQFYLSGMLFNNPDINMTFEQVYRDCKRGRGIYAAFQLENVVNVSDHFNIDQISEN
  INTELENLNVNIDSIELLDNTGRKSLEDFAHSGIDTIDYSTYLKETEKSPTEVNLLTFASTLEAKANQLP
  EGKLKQAFLLDVQNIRAIHQHLLPPVQQSLNTLRQSVWTLQQTSNKLPEKVKKILASLDSVQHFLTNNVS
  LIVIGETKKFGKTILGYFEHYLHWVFYAITEKMTSCKPMATAMDSAVNGILCGYVADPLNLFWFGIGKAT
  VLLLPAVIIAIKLAKYYRRMDSEDVYDDSSVSGMWHFTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163582
• ORF Size: 2427 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163582.1, NP_001157054.1
• RefSeq Size: 2653 bp
• RefSeq ORF: 2430 bp
• Locus ID: 19126
• UniProt ID: O54990
• Cytogenetics: 5 B3
• MW: 91.1 kDa
• Gene Summary: May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis (PubMed:19228982). Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.[UniProtKB/Swiss-Prot Function]