Ppfia1 (NM_001195086) Mouse Tagged ORF Clone

CAT#: MR225434

Ppfia1 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 (Ppfia1), transcript variant A

Product Images

Circular map for MR225434

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppfia1
• Synonyms: C030014K08Rik; C87158; LIP.1; LIP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225434 representing NM_001195086
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGCGAGGTGATGCCGACCATTAGTGAAGCAGAAGGCCCTCCAGGAGGAGGTGGGAGCCATGGGT
  CAGGCTCCCCTTCACAGCCAGATGCTGATTCGCATTTTGAGCAGCTCATGGTTTCCATGTTGGAAGAACG
  GGACCGCCTTCTGGACACACTAAGAGAGACACAAGAAACACTGGCATTAACCCAGGGGAAGTTGCATGAA
  GTTGGTCATGAAAGAGATTCTTTGCAGAGGCAGCTCAATACTGCACTTCCACAGGAGTTTGCTGCGCTCA
  CGAAGGAACTCAATGTGTGCAGGGAGCAGCTTCTAGAGAGAGAGGAAGAAATCGCCGAGCTGAAGGCAGA
  GAGGAACAACACTAGGCTGCTGTTGGAGCACCTAGAGTGCCTTGTCTCCAGGCATGAGCGCTCTCTCCGG
  ATGACAGTGGTAAAGAGGCAGGCACAGTCACCTGCAGGCGTGTCCAGTGAGGTGGAAGTGCTGAAAGCCC
  TGAAGTCACTGTTTGAACACCACAAAGCCTTGGATGAGAAGGTACGAGAGCGACTACGAGTGGCTCTGGA
  GAGGTGCAGCTTGTTAGAAGAGGAGCTGGGTGCCACCCACAAAGAGCTAATGATTCTTAAAGAACAGAAT
  AATCAGAAGAAAACACTAACAGATGGACTGCTTGACGGAAACCATGAACAGGAAAGTGCACCAAGCACCA
  ACGGCAAGAGATCTTCTGATGGCTCCTTGAGTCATGAGGATCTTGCCAAAGTGCTGGAGCTGCAGGAAGT
  CATAGACAGGCAGGCGAGAGAGCAGAGCCAGATGAAGGAGCGCCTGGCCTCCCTGTCCAGTCATGCAGCA
  GAACTAGAAGAGGATCTGGACACAGCTAGAAAAGACCTCATCAAGTCTGAAGAGATGAATACGAAACTGC
  AGCGAGAAGTCCGTGAAGCAATGGCCCAGAAGGAGGATATGGAAGAGAGAATCACCACCCTTGAGAAACG
  CTACCTCGCCGCACAGCGTGAAGCCACGTCTGTGCATGACCTCAATGACAAACTTGAAAATGAAATTGCA
  AATAAAGACTCCATGCACCGACAGACAGAAGACAAGAACCGCCAGTTGCAAGAGCGTCTGGAGTTGGCCG
  AGCAGAAGCTGCAGCAGACCCTGCGCAAAGCTGAGACGCTGCCAGAGGTGGAGGCTGAGCTGGCCCAGAG
  GGTGGCTGCACTCTCCAAGTCTGGTCCTTTGTCTTCTGGGAGTTCAGCTGCTAAGGAAGCAAAACTGTTG
  GAGCTTACTTCCAAGCTTAGGAAGGCTGAGGAGAGACATGGCAATATTGAAGAGAGGCTGCGGCAGATGG
  AGGCACAGCTGGAGGAGAAAAATCAGGAGCTGCAGCGGGCAAGGCAGAGAGAAAAGATGAACGAGGAACA
  TAATAAGCGCCTGTCTGACACTGTGGATAAGTTGCTCTCAGAATCCAATGAGAGGCTGCAGCTGCATCTG
  AAGGAGAGAATGGCGGCGCTGGAGGACAAGAATTCTCTACTAAGGGAAGTTGAAAATGCAAAGAAGCAAC
  TAGAAGAGACCCAGCATGATAAGGACCAACTTGTCGTGACTATTGAAGCCCTGAAGGCTGAGCTGGAGCA
  GATGAGACTGAGAGGTCCTTCACTCCATCATGGCCGACCCCATTTGGGCAGCGTCCCGGACTTCAGGTTC
  TCTGTGGCAGACGGACATGTGGATGCTTACAGCACCAGTGCAGTGTTGCGGCGCCCCCAGAAGGGCCGAC
  TGGCAGCGCTGCGGGATGAGCCCTCCAAGGTGCAGACCCTCAATGAGCAGGACTGGGAACGGGCCCAACA
  AGCCAGCGTCTTGGCAAATGTGGCACAGGCATTTGAGAGTGATGTCGATGTGTCTGATGGTGAAGATGAC
  CGAGATACACTGCTCAGCTCAGTTGATCTGCTGTCACCCAGTGGACAGGCTGATGCCCAGACACTGGCCA
  TGATGCTTCAGGAACAGCTGGATGCTATCAACAAAGAGATCAGGCTGATACAAGAAGAGAAGGAGAATAC
  AGAACAGCGGGCAGAGGAGATCGAGAGCAGAGTGGGCAGTGGAAGCTTGGACAATCTTGGTCGTTTTAGA
  TCAATGAGCTCCATTCCCCCCTACCCTGCTTCCTCGCTTGCTGGCTCCTCTCCTCCAGGCAGTGGTCGGT
  CCACACCAAGAAGGGTCCCTCACAGTCCTGCCCGGGAGGTGGACAGGCTGGGTGTCATGACTCTGCCTAG
  TGACTTAAGGAAGCACCGGAGACAGTTGCCAGCTTCCAGAGAAGAGGTACGAGACGACAAGACGACGATA
  AAATGTGAAACCTCTCCCCCCTCCTCCCCGAGGCCCCTGCGCTTAGACAGGATGCACAAGGGCGCACCGC
  ACACGGTCAGCCATGAGGACATCAGGGACCTGAGGAACTCCACAGGCTCCCAGGATGGTCCCGTGAGCAA
  CCCTAGCAGCAGTAATAGTAGCCAGGACTCCCTCCACAAAGCCCCAAAGAAGAAGGGTATCAAATCCTCC
  ATTGGCCGCTTGTTTGGGAAGAAGGAAAAGGGCCGGCCTGGTCCACTGGGCAAGGAGTCACCAGGGCAAG
  TTGGTGTTTCAGAGACAGAAAATTCATCTCAAGATGCCCTGGGACTCAGCAAACTGGGAGGACAAGCTGA
  GAAAAACCGTAAACTTCAGAAAAAGCACGAGCTGCTGGAGGAGGCCCGCAGGCAAGGCTTGCCTTTTGCA
  CAGTGGGATGGGCCCACAGTGGTAGTGTGGTTAGAGCTCTGGGTTGGGATGCCTGCCTGGTATGTGGCTG
  CTTGCCGAGCAAATGTCAAGAGTGGTGCCATCATGTCAGCCCTGTCAGACACTGAGATCCAGCGGGAGAT
  TGGCATCAGCAACCCCCTGCATAGGCTGAAGCTGCGGCTGGCCATCCAAGAGATAATGTCACTGACTAGC
  CCTTCTGCTCCACCCACATCCAGGACGACCACAGGAAATGTCTGGTTGACACATGAAGAGATGGAAACGC
  TTACAGCCACGCCACAAACGGAAGACGAGGAGGGAAGCTGGGCTCAGACTCTAGCATATGGGGACATGAA
  CCACGAGTGGATCGGCAATGAGTGGCTGCCCAGCCTGGGCCTGCCGCAGTACCGCAGCTACTTTATGGAG
  TGCCTTGTAGATGCCCGGATGCTGGACCACTTGACCAAGAAAGACTTGCGAGGGCAGCTGAAGATGGTCG
  ACAGCTTCCACAGAAACAGTTTCCAGTGTGGAATTATGTGCCTGAGAAGGCTAAATTATGACCGAAAAGA
  ACTGGAAAGAAAAAGAGAAGAGAGTCAGAATGAGACAAGAGATGTGCTTGTGTGGAGCAATGACCGGGTG
  ATTCGCTGGATCCTGTCGATTGGCCTTAAAGAGTATGCCAACAACCTAATAGAGAGCGGTGTTCACGGTG
  CACTTCTGGCTTTGGACGAGACTTTCGATTTCAGTGCCCTGGCACTCTTGTTACAGATTCCAACACAGAA
  TACGCAGGCTCGAGCTGTCTTGGAAAGAGAATTCAACAACCTTTTGATCACGGGGACTGATCGGAGGTTT
  GATGAGGATGATGACAAAAGCTTCAGGAGGGCGCCTTCCTGGAGGAAGAAGTTCAGACCAAAGGACATTC
  GTGGCTTGGCCTCTGGCTCTGCAGAGACGTTGCCTGCCAACTTCCGGGTCACCTCCTCAATGTCATCTCC
  CTCTATGCAGCCAAAGAAGGTTCAGATGGACGGTAGTGTGTCCGGAGCACAGAGGTTGGACTCTGCCACA
  GTCAGGACTTACTCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225434 representing NM_001195086
  Red=Cloning site Green=Tags(s)
  
  MMCEVMPTISEAEGPPGGGGSHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHE
  VGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLR
  MTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQN
  NQKKTLTDGLLDGNHEQESAPSTNGKRSSDGSLSHEDLAKVLELQEVIDRQAREQSQMKERLASLSSHAA
  ELEEDLDTARKDLIKSEEMNTKLQREVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIA
  NKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKSGPLSSGSSAAKEAKLL
  ELTSKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHL
  KERMAALEDKNSLLREVENAKKQLEETQHDKDQLVVTIEALKAELEQMRLRGPSLHHGRPHLGSVPDFRF
  SVADGHVDAYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDGEDD
  RDTLLSSVDLLSPSGQADAQTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFR
  SMSSIPPYPASSLAGSSPPGSGRSTPRRVPHSPAREVDRLGVMTLPSDLRKHRRQLPASREEVRDDKTTI
  KCETSPPSSPRPLRLDRMHKGAPHTVSHEDIRDLRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSS
  IGRLFGKKEKGRPGPLGKESPGQVGVSETENSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFA
  QWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTS
  PSAPPTSRTTTGNVWLTHEEMETLTATPQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFME
  CLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNETRDVLVWSNDRV
  IRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLITGTDRRF
  DEDDDKSFRRAPSWRKKFRPKDIRGLASGSAETLPANFRVTSSMSSPSMQPKKVQMDGSVSGAQRLDSAT
  VRTYSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195086
• ORF Size: 3798 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195086.1, NP_001182015.1
• RefSeq Size: 5259 bp
• RefSeq ORF: 3801 bp
• Locus ID: 233977
• Cytogenetics: 7 F5
• MW: 142.7 kDa