Cldn1 (NM_016674) Mouse Tagged ORF Clone

CAT#: MR225404

Cldn1 (Myc-DDK-tagged) - Mouse claudin 1 (Cldn1)

Product Images

Circular map for MR225404

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cldn1
• Synonyms: AI596271
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225404 representing NM_016674
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAACGCGGGGCTGCAGCTGCTGGGTTTCATCCTGGCTTCTCTGGGATGGATCGGCTCCATCGTCA
  GCACTGCCCTGCCCCAGTGGAAGATTTACTCCTATGCTGGGGACAACATCGTGACCGCTCAGGCCATCTA
  CGAGGGACTGTGGATGTCCTGCGTTTCGCAAAGCACCGGGCAGATACAGTGCAAAGTCTTCGATTCCTTG
  CTGAATCTGAACAGTACTTTGCAGGCAACCCGAGCCTTGATGGTAATTGGCATCCTGCTGGGGCTGATCG
  CAATCTTTGTGTCCACCATTGGCATGAAGTGCATGAGGTGCCTGGAAGATGATGAGGTGCAGAAGATGTG
  GATGGCTGTCATTGGGGGCATAATATTTTTAATTTCAGGTCTGGCGACATTAGTGGCCACAGCATGGTAT
  GGAAACAGAATTGTTCAAGAATTCTATGACCCCTTGACCCCCATCAATGCCAGGTATGAATTTGGCCAGG
  CCCTCTTTACTGGCTGGGCCGCTGCCTCCCTCTGCCTTCTGGGAGGTGTCCTACTTTCCTGCTCCTGTCC
  CCGGAAAACAACCTCTTACCCAACACCACGGCCTTATCCCAAGCCAACACCTTCTAGTGGGAAAGACTAT
  GTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225404 representing NM_016674
  Red=Cloning site Green=Tags(s)
  
  MANAGLQLLGFILASLGWIGSIVSTALPQWKIYSYAGDNIVTAQAIYEGLWMSCVSQSTGQIQCKVFDSL
  LNLNSTLQATRALMVIGILLGLIAIFVSTIGMKCMRCLEDDEVQKMWMAVIGGIIFLISGLATLVATAWY
  GNRIVQEFYDPLTPINARYEFGQALFTGWAAASLCLLGGVLLSCSCPRKTTSYPTPRPYPKPTPSSGKDY
  V
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016674
• ORF Size: 633 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016674.1, NM_016674.2, NM_016674.3, NM_016674.4, NP_057883.1
• RefSeq Size: 3263 bp
• RefSeq ORF: 636 bp
• Locus ID: 12737
• UniProt ID: O88551
• Cytogenetics: 16 B2
• MW: 23.3 kDa
• Gene Summary: This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The knockout mice lacking this gene die soon after birth as a consequence of dehydration from trandermal water loss, indicating that this gene is indispensable for creating and maintaining the epidermal barrier. The protein encoded by this gene also has gastric tumor suppressive activity, and is a key factor for hepatitis C virus (HCV) entry. [provided by RefSeq, Aug 2010]