Pms2 (NM_008886) Mouse Tagged ORF Clone

CAT#: MR225383

Pms2 (Myc-DDK-tagged) - Mouse postmeiotic segregation increased 2 (S. cerevisiae) (Pms2)

Product Images

Circular map for MR225383

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pms2
• Synonyms: AW555130; Pmsl2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225383 representing NM_008886
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAAACCGAAGGCGTGAGTACAGAATGTGCTAAGGCCATCAAGCCTATTGATGGGAAGTCAGTCC
  ATCAAATTTGTTCTGGGCAGGTGGTACTCAGTTTAAGCACCGCTGTGAAGGAGTTGATAGAAAATAGTGT
  AGATGCTGGTGCTACTACTATTGATCTAAGGCTTAAAGACTATGGGGTGGACCTCATTGAAGTTTCAGAC
  AATGGATGTGGGGTAGAAGAAGAAAACTTTGAAGGTCTAGCTCTGAAACATCACACATCTAAGATTCAAG
  AGTTTGCCGACCTCACGCAGGTTGAAACTTTTGGCTTTCGGGGGGAAGCTCTGAGCTCTCTGTGTGCACT
  AAGTGATGTCACTATATCTACCTGCCACGGGTCTGCAAGCGTTGGGACTCGACTGGTGTTTGACCATAAT
  GGGAAAATCACCCAGAAAACTCCCTACCCCCGACCTAAAGGAACCACAGTCAGTGTGCAGCACTTATTTT
  ATACACTACCCGTGCGTTACAAAGAGTTTCAGAGGAACATTAAAAAGGAGTATGCCAAAATGGTGCAGGT
  CTTACAGGCGTACTGTATCATCTCAGCAGGCGTCCGTGTAAGCTGCACTAATCAGCTCGGACAGGGGAAG
  CGGCAGCCTGTGGTGTGCACAAGCGGCAGCTCTGGCATGAAGGAAAATATCGGGTCTGTGTTTGGCCAGA
  AGCAGTTGCAAAGCCTCATTCCTTTTGTTCAGCTGCCCCCTAGTGACGCTGTGTGTGAAGAGTACGGCCT
  GAGCACTTCCAGGACGCCACAAAACCTTTTCTACGTTTCGGGCTTCATTTCACAGTGCACGCACGGCGCC
  GGGAGGAGTGCAACAGACAGGCAGTTTTTCTTCATCAATCAGAGGCCCTGTGACCCAGCAAAGGTCTCTA
  AGCTTGTCAATGAGGTTTATCACATGTATAACCGGCATCAGTACCCATTTGTCGTCCTTAACGTTTCCGT
  TGACTCAGAATGTGTGGATATTAATGTAACTCCAGATAAAAGGCAAATTCTACTACAAGAAGAGAAGCTA
  TTGCTGGCCGTTTTAAAGACCTCCTTGATAGGAATGTTTGACAGTGATGCAAACAAGCTTAATGTCAACC
  AGCAGCCACTGCTAGATGTTGAAGGTAACTTAGTAAAGCTGCATACTGCAGAACTAGAAAAGCCTGTGCC
  AGGAAAGCAAGATAACTCTCCTTCACTGAAGAGCACAGCAGACGAGAAAAGGGTAGCATCCATCTCCAGG
  CTGAGAGAGGCCTTTTCTCTTCATCCTACTAAAGAGATCAAGTCTAGGGGTCCAGAGACTGCTGAACTGA
  CACGGAGTTTTCCAAGTGAGAAAAGGGGCGTGTTATCCTCTTATCCTTCAGACGTCATCTCTTACAGAGG
  CCTCCGTGGCTCGCAGGACAAATTGGTGAGTCCCACGGACAGCCCTGGTGACTGTATGGACAGAGAGAAA
  ATAGAAAAAGACTCAGGGCTCAGCAGCACCTCAGCTGGCTCTGAGGAAGGGTTCAGCACCCCAGAAGTGG
  CCAGTAGCTTTAGCAGTGACTATAACGTGAGCTCCCCAGAAGACAGACCTTCTCAGGAAACCATAAACTG
  TGGTGACTTGGACTGCCGTCCTCCAGGTACAGGACAGTCCTTGAAGCCAGAAGACCATGGATATCAATGC
  AAAGCTCTACCTCTAGCTCGTCTGTCACCCACAAATGCCAAGCGCTTCAAGACAGAGGAAAGACCCTCAA
  ATGTCAACATTTCTCAAAGATTGCCTGGTCCTCAGAGCACCTCAGCAGCTGAGGTCGATGTAGCCATAAA
  AATGAATAAGAGAATCGTGCTCCTCGAGTTCTCTCTGAGTTCTCTAGCTAAGCGAATGAAGCAGTTACAG
  CACCTAAAGGCGCAGAACAAACATGAACTGAGTTACAGAAAATTTAGGGCCAAGATTTGCCCTGGAGAAA
  ACCAAGCAGCAGAAGATGAACTCAGAAAAGAGATTAGTAAATCGATGTTTGCAGAGATGGAGATCTTGGG
  TCAGTTTAACCTGGGATTTATAGTAACCAAACTGAAAGAGGACCTCTTCCTGGTGGACCAGCATGCTGCG
  GATGAGAAGTACAACTTTGAGATGCTGCAGCAGCACACGGTGCTCCAGGCGCAGAGGCTCATCACACCCC
  AGACTCTGAACTTAACTGCTGTCAATGAAGCTGTACTGATAGAAAATCTGGAAATATTCAGAAAGAATGG
  CTTTGACTTTGTCATTGATGAGGATGCTCCAGTCACTGAAAGGGCTAAATTGATTTCCTTACCAACTAGT
  AAAAACTGGACCTTTGGACCCCAAGATATAGATGAACTGATCTTTATGTTAAGTGACAGCCCTGGGGTCA
  TGTGCCGGCCCTCACGAGTCAGACAGATGTTTGCTTCCAGAGCCTGTCGGAAGTCAGTGATGATTGGAAC
  GGCGCTCAATGCGAGCGAGATGAAGAAGCTCATCACCCACATGGGTGAGATGGACCACCCCTGGAACTGC
  CCCCACGGCAGGCCAACCATGAGGCACGTTGCCAATCTGGATGTCATCTCTCAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225383 representing NM_008886
  Red=Cloning site Green=Tags(s)
  
  MEQTEGVSTECAKAIKPIDGKSVHQICSGQVVLSLSTAVKELIENSVDAGATTIDLRLKDYGVDLIEVSD
  NGCGVEEENFEGLALKHHTSKIQEFADLTQVETFGFRGEALSSLCALSDVTISTCHGSASVGTRLVFDHN
  GKITQKTPYPRPKGTTVSVQHLFYTLPVRYKEFQRNIKKEYAKMVQVLQAYCIISAGVRVSCTNQLGQGK
  RQPVVCTSGSSGMKENIGSVFGQKQLQSLIPFVQLPPSDAVCEEYGLSTSRTPQNLFYVSGFISQCTHGA
  GRSATDRQFFFINQRPCDPAKVSKLVNEVYHMYNRHQYPFVVLNVSVDSECVDINVTPDKRQILLQEEKL
  LLAVLKTSLIGMFDSDANKLNVNQQPLLDVEGNLVKLHTAELEKPVPGKQDNSPSLKSTADEKRVASISR
  LREAFSLHPTKEIKSRGPETAELTRSFPSEKRGVLSSYPSDVISYRGLRGSQDKLVSPTDSPGDCMDREK
  IEKDSGLSSTSAGSEEGFSTPEVASSFSSDYNVSSPEDRPSQETINCGDLDCRPPGTGQSLKPEDHGYQC
  KALPLARLSPTNAKRFKTEERPSNVNISQRLPGPQSTSAAEVDVAIKMNKRIVLLEFSLSSLAKRMKQLQ
  HLKAQNKHELSYRKFRAKICPGENQAAEDELRKEISKSMFAEMEILGQFNLGFIVTKLKEDLFLVDQHAA
  DEKYNFEMLQQHTVLQAQRLITPQTLNLTAVNEAVLIENLEIFRKNGFDFVIDEDAPVTERAKLISLPTS
  KNWTFGPQDIDELIFMLSDSPGVMCRPSRVRQMFASRACRKSVMIGTALNASEMKKLITHMGEMDHPWNC
  PHGRPTMRHVANLDVISQN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008886
• ORF Size: 2577 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008886.2, NP_032912.2
• RefSeq Size: 3273 bp
• RefSeq ORF: 2580 bp
• Locus ID: 18861
• Cytogenetics: 5 G2
• MW: 95.7 kDa