Fgfr2 (NM_201601) Mouse Tagged ORF Clone

CAT#: MR225121

Fgfr2 (Myc-DDK-tagged) - Mouse fibroblast growth factor receptor 2 (Fgfr2), transcript variant 2

Product Images

Circular map for MR225121

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fgfr2
• Synonyms: AU043015; AW556123; Bek; Fgfr-2; Fgfr-7; Fgfr7; KGFR; KGFRTr; svs
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225121 representing NM_201601
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATTACCGTCCACGTGGAGATATGGAAGAGGACCAGGGATTGGCACTGTGACCATGGTCAGCTGGG
  GGCGCTTCATCTGCCTGGTCTTGGTCACCATGGCAACCTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGT
  TGAGGATACCACTTTAGAACCAGAAGGAGCACCGTACTGGACCAACACCGAGAAGATGGAGAAGCGGCTC
  CACGCTGTCCCTGCCGCCAACACTGTGAAGTTCCGCTGTCCGGCTGGGGGGAATCCAACGCCCACAATGA
  GGTGGTTAAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTATAAGGTACGAAACCAGCA
  CTGGAGCCTTATTATGGAAAGTGTGGTCCCGTCAGACAAAGGCAACTACACCTGCCTGGTGGAGAATGAA
  TACGGGTCCATCAACCACACCTACCACCTCGATGTCGTTGAACGGTCACCACACCGGCCCATCCTCCAAG
  CTGGACTGCCTGCAAATGCCTCCACGGTGGTCGGAGGGGATGTGGAGTTTGTCTGCAAGGTTTACAGCGA
  TGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCTGATGGGCTG
  CCCTACCTCAAGGTCCTGAAGCACTCGGGGATAAATAGCTCCAATGCAGAAGTGCTGGCTCTGTTCAATG
  TGACGGAGATGGATGCTGGGGAATATATATGTAAGGTCTCCAATTATATAGGGCAGGCCAACCAGTCTGC
  CTGGCTCACTGTCCTGCCCAAACAGCAAGCGCCTGTGAGAGAGAAGGAGATCACGGCTTCCCCAGATTAT
  CTGGAGATAGCTATTTACTGCATAGGGGTCTTCTTAATCGCCTGCATGGTGGTGACAGTCATCTTTTGCC
  GAATGAAGACCACGACCAAGAAGCCAGACTTCAGCAGCCAGCCAGCTGTGCACAAGCTGACCAAGCGCAT
  CCCCCTGCGGAGACAGGTAACAGTTTCGGCCGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTG
  AGGATAACAACGCGTCTGTCCTCAACAGCGGACACCCCGATGCTAGCAGGGGTCTCCGAGTATGAGTTGC
  CAGAGGATCCAAAGTGGGAATTCCCCAGAGATAAGCTGACGCTGGGCAAACCCCTGGGGGAAGGTTGCTT
  CGGGCAAGTAGTCATGGCTGAAGCAGTGGGAATCGATAAAGACAAACCCAAGGAGGCGGTCACCGTGGCA
  GTGAAGATGTTGAAAGATGATGCCACAGAGAAGGACCTGTCTGATCTGGTATCAGAGATGGAGATGATGA
  AGATGATTGGGAAACATAAGAACATTATCAACCTCCTGGGGGCCTGCACGCAGGATGGACCTCTCTACGT
  CATAGTTGAATATGCATCGAAAGGCAACCTCCGGGAATACCTCCGAGCCCGGAGGCCACCTGGCATGGAG
  TACTCCTATGACATTAACCGTGTCCCCGAGGAGCAGATGACCTTCAAGGACTTGGTGTCCTGCACCTACC
  AGCTGGCTAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATCCATCGAGATTTGGCTGCCAGAAACGT
  GTTGGTAACAGAAAACAATGTGATGAAGATAGCAGACTTTGGCCTGGCCAGGGATATCAACAACATAGAC
  TACTATAAAAAGACCACAAATGGGCGACTTCCAGTCAAGTGGATGGCTCCTGAAGCCCTTTTTGATAGAG
  TTTACACTCATCAGAGCGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTTACTTTAGGGGGCTC
  ACCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTCAAAGAGGGACACAGGATGGACAAGCCC
  ACCAACTGCACCAATGAACTGTACATGATGATGAGGGATTGCTGGCATGCTGTACCCTCACAGAGACCCA
  CATTCAAGCAGTTGGTCGAAGACTTGGATCGAATTCTGACTCTCACAACCAATGAGGAATACTTGGATCT
  CACCCAGCCTCTCGAACAGTATTCTCCTAGTTACCCCGACACAAGGAGCTCTTGTTCTTCAGGGGACGAT
  TCTGTGTTTTCTCCAGACCCCATGCCTTATGAACCCTGTCTGCCTCAGTATCCACACATAAACGGCAGTG
  TTAAAACA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225121 representing NM_201601
  Red=Cloning site Green=Tags(s)
  
  MGLPSTWRYGRGPGIGTVTMVSWGRFICLVLVTMATLSLARPSFSLVEDTTLEPEGAPYWTNTEKMEKRL
  HAVPAANTVKFRCPAGGNPTPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCLVENE
  YGSINHTYHLDVVERSPHRPILQAGLPANASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNGSKYGPDGL
  PYLKVLKHSGINSSNAEVLALFNVTEMDAGEYICKVSNYIGQANQSAWLTVLPKQQAPVREKEITASPDY
  LEIAIYCIGVFLIACMVVTVIFCRMKTTTKKPDFSSQPAVHKLTKRIPLRRQVTVSAESSSSMNSNTPLV
  RITTRLSSTADTPMLAGVSEYELPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVA
  VKMLKDDATEKDLSDLVSEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLRARRPPGME
  YSYDINRVPEEQMTFKDLVSCTYQLARGMEYLASQKCIHRDLAARNVLVTENNVMKIADFGLARDINNID
  YYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLLKEGHRMDKP
  TNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRILTLTTNEEYLDLTQPLEQYSPSYPDTRSSCSSGDD
  SVFSPDPMPYEPCLPQYPHINGSVKT
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201601
• ORF Size: 2178 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201601.2, NP_963895.2
• RefSeq Size: 4881 bp
• RefSeq ORF: 2181 bp
• Locus ID: 14183
• UniProt ID: P21803
• Cytogenetics: 7 F3
• MW: 82.1 kDa
• Gene Summary: Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.[UniProtKB/Swiss-Prot Function]