Synm (NM_207663) Mouse Tagged ORF Clone

CAT#: MR224870

Synm (Myc-DDK-tagged) - Mouse synemin, intermediate filament protein (Synm), transcript variant 2

Product Images

Circular map for MR224870

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Synm
• Synonyms: 4930412K21Rik; AI852401; Dmn; E130104F11; Syn; Synemin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224870 representing NM_207663
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCCTGGCGGCTGCAGACGGGCTCTGAAAAGGCGGAGCTGCAGGAGCTCAACGCCCGGCTTTACG
  ACTACGTGTGCCGGGTGCGAGAGCTGGAGCGCGAGAACCTGCTACTGGAGGAGGAGTTGCGCAGCCGGCT
  GAGCCGGGAGGACCGCTGGGCTGAGGATCAGGCACTCTACGCCGAGGAGGCGCGCAGCCTACGACAGCAG
  CTAGATGAACTGAACTGGTCCACAGCCCTGGCCGAGGGCGAGCGCGACGCGCTGCGGAGGGAACTGCTGG
  AGCTGCAGCGGGAGGGTGTGGAAGCCGGCACTGCCCGCAGCCGCCTGGATGCCGAGCTGGGAGCGCAACG
  GCGGGAGCTAGAGGAGGCTCTGGGCGCGCGCGCCGCCCTCGAGGCGCTGCTGGGCCGGCTGGAAACTGAG
  CGCCGCGACTTGGACGCTGCCCACGAACGCCAAGTGCGAGATCTACGAGCCCGCGCTGCCAGCCTCACCA
  TGCACTTCCGAGCCCGCGCCACCAGCCCCGCCGCGCCCCCGCCGCGCCTGCGGGACGTGCACGACAGCTA
  CGCCCTGCTGGTGGCCGAGTCGTGGAGGGAGAGCGTGCAGCTGTACGAGGACGAGGTGCGCGAGCTGGAG
  CAGGCGCTGCGCCGCGGCCAAGAGAGCCGGCTCCAGGCGGAGGACGAGGCGCGGCTGTGCGCGCAGGAGG
  CAGACGCGCTGCGGAACCAGGCGCTGGAGTTGGAGCAGCTGCGCGCGAGGCTGGAGGATGAGCTGCTGCG
  GATGCGCGAGGAGTACGGGATGCAGGCGGAGGAGCGGCAGAGAGTGATTGACAGCCTGGAGGATGAGAAG
  GAAGCCCTTACCTTGGCCATGGCTGACCGGCTGCGGGATTACCAGGAGCTCCTGCAGGTGAAGACTGGAC
  TCAGCTTGGAGGTAGCCACCTACAGGGCCTTGTTGGAAGGAGAAAGCAATCCGGAGATATTGATCTGGAC
  TGAGAACATTGAAAACGTGCCACAAGAGCCCAGAAACACATCCTATCGCTACACCAACTCAGTATTGCAG
  AGGAAAAATGAGAAAAATCTCTTTCCAAGGCGAAAAACACCTTGGGCAGCTGTAAATCACAGCTCGGCCT
  CGTATTCTAACTGGCCAGGGCACCTTGACTCACAGACAACCACAGCTGTAGGAAGTGCTGCCAGAAGGGG
  TCTCCTGACCTCCCGCCATTCTTCCTCAGCTACAACCTCAGGGCAGCAAAAGCCCCTGGAGAAAACCATC
  AGCAGCCGAGCCAATTTAAGACCGGTCACGCCAACCCACGGCTTTCTAAGAAACACTGATGCTCAAATGA
  AAACACTCCCCCATAGATCCAAAGTCGAAGGCACGGGGGACACCCACGCACGGCGGGCTACAGAGTCTGT
  TATTACCAGAGAGTCATACAGAGGTCACCAGGGCCATGTGGCAGCAGGTGCCGTGAGCAGCACTCCGTCA
  AATGAGAGGACTGTCATTCTGGGAAAGAAATTAGAAGCGCAAGCCACTAAAGAACAAGAAAGGGACAGAT
  CAGGGGTCATCAGAATAAAGCCAGAAGAGAAAATGTTTGATTCTAAAGAGAAGGCTTCAGAGGAAAGAAA
  CTTGAGGTGGGAAGAGCTAACCAAGTTAGATAGAGATGCCAGAAAGAGAGAAAGCCGGCATTTGAGGGAT
  GAGGCCAGAGAGAAGGAAGCACTGAAGGAGAGAAGTGTAAAAGAAAGAGAGGTGCCCATCAGTCTAGAAG
  TATCCCGGGGCAGCAGAGCAGAGGTGTCCACCATACACTTGCAGTCACCTGGAAGGAAGGACGTTAGCCA
  CAGTGGGGGAAGAGAGGCAGAGACCAAAGAGACAAGGTTCCGGCTGGATACCCAGGACACTGCCAGCTCT
  CTGCAAAGTGACTCAACGACAGAAACCATAGCCGAAAGCATTGTCACCACCATCCTTAAGCAGTTTACCC
  AGTCTCCAGGTGCCGAGGAGGAGGCCACTTCTTTCCCGGACACAAAGGTCACTTATGTGGACAGGAAAGA
  GTTTCCTGGTGATGGGAAAACCAAGACAGAAATCGTTGTGGAGTCGAAGCTGACAGATGTTGTTGATGTT
  TCTGATGAAGCAGGCCTGGACTACCTTCTAAGCAAGGATGTCAAGGAAGTAGGACTGAAGGGGAAGTCAA
  CGGAGACGATGATCGGAGAGATGATCAACCTGGGTCTGAAAGGCAGGGAGGGGAGAGCTAAAGTCGTAAA
  TGTGGAGATTGTGGAAGAGCCCATGAGCTACATAGGTGGTGGGAAAATAGACTTTTCTACCCCCTTTCAG
  GTAGAAGAAGTCGACGACGTGTCTCCCAGCCCCAAGGGCTTTGTGGAGGAAGAGGATGGTGAAGGAGAGA
  CCCACATGGCATTCTCAATGCGTCCACATCAGACCAAACAGCCCCAGGGGACCATCCCTCACGTGGAAGA
  AGTGACGGAGGCGGGCGACTCAGAGGGGGAGCAGAGCTATTTTGTGTCTACCCCAGATGAGTACCCCGGG
  GGGCATGACAGAGAAGATGATGGCTCAGTGTATGGGCAGATTCATATCGAGGAAGAGTCTACCATCCGGT
  ACTCATGGCAAGACGAAATTGCGCAGGGGACCTGGAGAAGGAAAATGAGAGGTGACGTGGGGGGAGAGAA
  GCCTGTGAAGGTCCTGGAGGTCCCAGCACTTTCCCTGGGTGGTGCTATTGGTTCTGCTCACTTGAAGGAA
  GAAGCCAGTGGTGAACTCCGTGCTGAACCCACAGTCATTGAGAAAGAGATCAAAATACCGCATGAATTCC
  ACACCTCCATCAAGGGTGTCTTCTCCAGTGAGCCCCGGCACCAGCTGGTGGAGGTCATCGGGCAGCTGGA
  GGAAACATTGCCCGAGCGCATGAAGGAGGAGCTGTCTGCCCTGACCAGACAGAGTCAAGGAGAGTCAGGG
  AGCGTTTCTGTGGACGTAAAGAAAGTCCAGAGCGCTGCTGGTGGTTCTGTGACTTTGATGGCTGAGGTCA
  ACCTCTCACAGACTGTAGATGCTGATCAGTTGGACCTGGAGCAGTTGAGCAGAGATGAAGCTGGGGAAAT
  AGAGAGGGCTGTGGAGTCTGTGGTCAGAGAGAGCTTGGCCAAGCGCTCCAGCCCAGTGCCTAGAAGCCCA
  GACAGGGAAGATGGAGAGGAGGTGCCTGCTGGTGGCATTCTCTTCAAGCGCTGGGCCACCAGGGAGCTGT
  ATAGCCCATCTGGTGAGAGGGATGATGCTGGCCAGGTCTCTCCCAGCTCAGATCAGCGTGTTACCCAGGG
  CCCAGTGTCAGCTACTGTGGAAGTGACCAGCCCAACGGGTTTTGTACAGTCTCATGTGCTGGAGGATGTG
  AGCCAGTCTGTAAGGCATGTTAAACTAGGTCCCACTGAAATGTGGAGGACAGAACAGGTGACCTTCGGAG
  GACCCACCGCACAGGTGGTGGAGATGGATCTGAGCGACACGAGAGCGATCCGCAGCTGGACCCGAGACAC
  AGGGTCGGAAGTGGAAGCTCACGGCGTGTCTCACCGTGGTGGCTGGAGAATTGCACACAGTAGGGATGAG
  CGAGTGGCCAGCACTGGCTCTGGGGCCTCCCCTGGGGATGCACACCAGGCCCCTGGAGAGAAAGGCACGG
  AGCAGGCTGGATTTGACAAGACGGTACAGTTGCAGAGAATGGTAGACCAAAGGTCGGTGGCCTCAGATGA
  GAAGAAAGTGGCTCTTCTTTATCTAGACAATGAAGAGGAGGAGGAGGAGGAGGGCGAAGGGTGGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224870 representing NM_207663
  Red=Cloning site Green=Tags(s)
  
  MLSWRLQTGSEKAELQELNARLYDYVCRVRELERENLLLEEELRSRLSREDRWAEDQALYAEEARSLRQQ
  LDELNWSTALAEGERDALRRELLELQREGVEAGTARSRLDAELGAQRRELEEALGARAALEALLGRLETE
  RRDLDAAHERQVRDLRARAASLTMHFRARATSPAAPPPRLRDVHDSYALLVAESWRESVQLYEDEVRELE
  QALRRGQESRLQAEDEARLCAQEADALRNQALELEQLRARLEDELLRMREEYGMQAEERQRVIDSLEDEK
  EALTLAMADRLRDYQELLQVKTGLSLEVATYRALLEGESNPEILIWTENIENVPQEPRNTSYRYTNSVLQ
  RKNEKNLFPRRKTPWAAVNHSSASYSNWPGHLDSQTTTAVGSAARRGLLTSRHSSSATTSGQQKPLEKTI
  SSRANLRPVTPTHGFLRNTDAQMKTLPHRSKVEGTGDTHARRATESVITRESYRGHQGHVAAGAVSSTPS
  NERTVILGKKLEAQATKEQERDRSGVIRIKPEEKMFDSKEKASEERNLRWEELTKLDRDARKRESRHLRD
  EAREKEALKERSVKEREVPISLEVSRGSRAEVSTIHLQSPGRKDVSHSGGREAETKETRFRLDTQDTASS
  LQSDSTTETIAESIVTTILKQFTQSPGAEEEATSFPDTKVTYVDRKEFPGDGKTKTEIVVESKLTDVVDV
  SDEAGLDYLLSKDVKEVGLKGKSTETMIGEMINLGLKGREGRAKVVNVEIVEEPMSYIGGGKIDFSTPFQ
  VEEVDDVSPSPKGFVEEEDGEGETHMAFSMRPHQTKQPQGTIPHVEEVTEAGDSEGEQSYFVSTPDEYPG
  GHDREDDGSVYGQIHIEEESTIRYSWQDEIAQGTWRRKMRGDVGGEKPVKVLEVPALSLGGAIGSAHLKE
  EASGELRAEPTVIEKEIKIPHEFHTSIKGVFSSEPRHQLVEVIGQLEETLPERMKEELSALTRQSQGESG
  SVSVDVKKVQSAAGGSVTLMAEVNLSQTVDADQLDLEQLSRDEAGEIERAVESVVRESLAKRSSPVPRSP
  DREDGEEVPAGGILFKRWATRELYSPSGERDDAGQVSPSSDQRVTQGPVSATVEVTSPTGFVQSHVLEDV
  SQSVRHVKLGPTEMWRTEQVTFGGPTAQVVEMDLSDTRAIRSWTRDTGSEVEAHGVSHRGGWRIAHSRDE
  RVASTGSGASPGDAHQAPGEKGTEQAGFDKTVQLQRMVDQRSVASDEKKVALLYLDNEEEEEEEGEGWF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207663
• ORF Size: 3777 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207663.3, NP_997546.2
• RefSeq Size: 6960 bp
• RefSeq ORF: 3780 bp
• Locus ID: 233335
• UniProt ID: Q70IV5
• Cytogenetics: 7 C
• MW: 141.3 kDa
• Gene Summary: Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells (By similarity).[UniProtKB/Swiss-Prot Function]