Uspl1 (NM_001115151) Mouse Tagged ORF Clone

CAT#: MR224853

Uspl1 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase like 1 (Uspl1), transcript variant 5

Product Images

Circular map for MR224853

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Uspl1
• Synonyms: E430026A01Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224853 representing NM_001115151
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCTAAGAGCAGATGTTTACCACTTTGCCAGACTCTGTGTGTCCAGTGGAAAAACACCCAGGCCC
  TGTGCTGGTTAGACTGTATCCTGTCAGCCCTGGTGCACTTGGAGGTGTTGAGGAAGACTGTGCTGGAGGC
  GTGCTCCAGGGAGGAGTGTGTATTTGGAAGGCTGTTTGAAATGTACCACCAAGCAGATGAGCTGCTGCAC
  ACCCATCACCTGCATGGCGTCACAGGTGAAGATTGTAAAAAATTGACATCAGAAATATTTACAGAAATAG
  ATACCTGTTTGAATAAAGTTAGAGATGAAATTTTTGCTAAACTTCAACCGAAGCTTAGATGCACATTAGG
  TGACATGGAAAGTCCTGTGTTTGCACTTCCTGTACTGTTAAAGCTTGAACCCCATGTTGAAAGCCTCTTT
  ACATATTCTTTTTCTTGGAATTTTGAATGTTCCCATTGTGGACACCAGTACCAAAACAGGTGTGTGAAGA
  GTCTGGTCACCTTTACCAATATTGTTCCTGAGTGGCATCCACTCAATGCTGCCCATTTTGGTCCATGTAA
  CAGCTGCAACAGTAAATCACAAATAAGAAAAATGGTGTTGGAAAGAGCGTCGCCCATACTCATGCTGCAC
  TTCGTGGAGGGCTTACCAAGGAGGGATTTGCAACACTATGCCTTCCACTTTGAAGGAAGCCTTTATCAAG
  TCACGTCGGTAATACAGTACCAAGCAAATAACCATTTTATAACATGGTTTTTAGATGCTGATGGGAGTTG
  GCTGGAGTGTGATGACCTAAAGGGTCCCTGTGCTAAAAGGCACGTGACCTGTGAAGTGCCAGCTTCAGAG
  ACACATATCGTTATTTGGGAAAGAAAATCCCAGGTGCCAATTGAAGAAGCTGCCTGCCTTCCATGCATGA
  AGCCAAATGTGCAGCCTGTATCAGGAATTGAAGTCCCTGGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224853 representing NM_001115151
  Red=Cloning site Green=Tags(s)
  
  MPAKSRCLPLCQTLCVQWKNTQALCWLDCILSALVHLEVLRKTVLEACSREECVFGRLFEMYHQADELLH
  THHLHGVTGEDCKKLTSEIFTEIDTCLNKVRDEIFAKLQPKLRCTLGDMESPVFALPVLLKLEPHVESLF
  TYSFSWNFECSHCGHQYQNRCVKSLVTFTNIVPEWHPLNAAHFGPCNSCNSKSQIRKMVLERASPILMLH
  FVEGLPRRDLQHYAFHFEGSLYQVTSVIQYQANNHFITWFLDADGSWLECDDLKGPCAKRHVTCEVPASE
  THIVIWERKSQVPIEEAACLPCMKPNVQPVSGIEVPGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001115151
• ORF Size: 954 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001115151.1, NP_001108623.1
• RefSeq Size: 3103 bp
• RefSeq ORF: 957 bp
• Locus ID: 231915
• UniProt ID: Q3ULM6
• Cytogenetics: 5 G3
• MW: 36.8 kDa
• Gene Summary: SUMO-specific isopeptidase involved in protein desumoylation. Specifically binds SUMO proteins with a higher affinity for SUMO2 and SUMO3 which it cleaves more efficiently. Also able to process full-length SUMO proteins to their mature forms (By similarity). Plays a key role in RNA polymerase-II-mediated snRNA transcription in the Cajal bodies (By similarity). Is a component of complexes that can bind to U snRNA genes (By similarity).[UniProtKB/Swiss-Prot Function]