Ror1 (NM_013845) Mouse Tagged ORF Clone

CAT#: MR224803

Ror1 (Myc-DDK-tagged) - Mouse receptor tyrosine kinase-like orphan receptor 1 (Ror1)

Product Images

Circular map for MR224803

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ror1
• Synonyms: 2810404D04Rik; Ntr; Ntrkr1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224803 representing NM_013845
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCGGCCGCGCCGCCGCGGGACGCGCCCGCCACCGCTGGCGCTGCTGGCCGCGCTGCTGCTGGCCG
  CACGCGGGGCTGATGCCCAAGAAACAGAGTTGTCAGTCAGTGCTGAGCTGGTGCCTACCTCGTCCTGGAA
  CACTTCAAGTGAAATCGACAAAGGTTCTTACTTAACCCTTGATGAGCCGATGAATAACATCACAACGTCC
  CTGGGGCAGACTGCAGAACTGCACTGCAAAGTGTCTGGGAATCCACCTCCCAGTATCCGCTGGTTCAAGA
  ATGATGCACCTGTGGTCCAAGAACCTCGGAGAATCTCCTTCCGGGCAACCAACTATGGCTCTCGGCTGCG
  GATTAGAAACCTTGACACCACAGACACTGGTTACTTCCAGTGTGTGGCAACAAATGGCAAGAAAGTGGTG
  TCTACCACTGGTGTCCTGTTTGTCAAATTTGGGCCTCCTCCGACCGCAAGCCCAGGATCCTCAGATGAGT
  ATGAAGAAGATGGATTCTGTCAGCCGTACCGAGGCATTGCATGTGCACGATTTATTGGCAACCGCACTGT
  GTATATGGAGTCTTTGCATATGCAGGGGGAAATAGAAAATCAGATCACAGCTGCCTTCACCATGATTGGC
  ACCTCCAGCCATTTATCTGATAAGTGCTCTCAGTTCGCCATCCCTTCCTTGTGCCACTACGCCTTCCCGT
  ACTGTGACGAAACCTCATCTGTCCCAAAGCCCCGTGACTTGTGTCGTGATGAATGTGAAGTGCTGGAGAA
  TGTCCTGTGTCAGACAGAGTACATTTTTGCCAGATCAAATCCCATGATTTTGATGAGGCTGAAGTTACCA
  AACTGTGAGGATCTCCCCCAGCCAGAGAGCCCGGAAGCTGCAAACTGCATACGGATTGGCATTCCCATGG
  CGGATCCTATAAATAAAAATCACAAATGCTACAATAGCACGGGTGTAGACTACCGGGGAACCGTCAGTGT
  GACCAAGTCTGGACGCCAGTGCCAGCCATGGAATTCTCAGTACCCACACACACACAGCTTCACTGCTCTG
  CGCTTTCCGGAGCTCAACGGAGGCCACTCCTACTGCCGCAACCCTGGCAACCAGAAGGAAGCTCCCTGGT
  GCTTTACCTTGGATGAAAACTTTAAGTCTGACCTGTGTGACATCCCAGCATGTGATTCCAAAGATTCCAA
  AGAGAAGAATAAAATGGAAATCTTGTACATTCTGGTGCCAAGTGTGGCCATTCCCCTGGCTATCGCCTTC
  CTCTTCTTCTTCATCTGTGTGTGCCGCAATAACCAGAAGTCTTCATCACCACCAGTCCAGAGGCAGCCAA
  AACCCGTCAGAGGACAGAATGTGGAGATGTCCATGCTCAATGCATACAAGCCCAAGAGCAAGGCTAAAGA
  GCTGCCTCTTTCCGCTGTGCGTTTCATGGAAGAATTGGGTGAATGTACCTTTGGAAAAATCTATAAGGGC
  CATCTCTACCTCCCAGGCATGGACCATGCTCAGCTGGTGGCTATCAAAACCTTGAAAGACTATAACAACC
  CCCAGCAGTGGACAGAATTTCAACAGGAAGCCTCTCTCATGGCTGAACTACACCACCCCAATATTGTATG
  CCTCCTCGGAGCCGTCACCCAGGAACAACCTGTGTGTATGCTCTTTGAGTATATGAACCAGGGAGACCTC
  CACGAGTTCCTCATCATGCGATCCCCGCATTCCGATGTCGGCTGTAGCAGTGATGAAGATGGGACGGTCA
  AATCCAGCCTGGACCATGGTGATTTCTTACACATAGCAATTCAGATCGCAGCTGGCATGGAGTACCTGTC
  TAGTCACTTCTTCGTGCACAAGGACCTTGCAGCTCGCAACATTTTAATTGGAGAGCAACTGCATGTAAAA
  ATTTCAGATCTTGGGCTTTCCAGAGAAATTTACTCTGCTGATTACTATAGGGTACAGAGTAAGTCTTCAC
  TGCCCATCCGCTGGATGCCCCCTGAGGCCATCATGTACGGCAAATTCTCCTCCGATTCCGATATCTGGTC
  TTTCGGGGTTGTGTTGTGGGAGATTTTCAGCTTTGGACTCCAGCCATATTATGGGTTTAGTAATCAGGAA
  GTGATTGAAATGGTGCGGAAGCGGCAGCTCTTACCATGTTCTGAAGACTGCCCGCCGCGCATGTACAGCC
  TCATGACCGAGTGCTGGAATGAGATACCTTCCAGGAGACCACGCTTTAAAGACATCCACGTCCGGCTTCG
  ATCCTGGGAGGGCCTCTCAAGTCACACCAGCTCTACCACCCCCTCGGGTGGAAATGCCACCACGCAGACC
  ACTTCCCTCAGTGCCAGCCCTGTGAGTAACCTCAGCAACCCCCGATTTCCCAATTACATGTTCCCGAGCC
  AAGGGATTACACCCCAGGGTCAGATCGCTGGTTTCATTGGCCCAGCGATACCTCAGAACCAGCGCTTCAT
  CCCCATCAATGGATACCCAATACCTCCTGGCTATGCAGCCTTTCCAGCTGCCCACTACCAGCCTGCAGGG
  CCTCCCAGGGTGATTCAGCACTGCCCACCTCCGAAGAGTCGGTCCCCAAGCAGCGCCAGCGGATCGACCA
  GCACTGGCCATGTGGCCAGCTTGCCCTCATCAGGATCCAATCAGGAAGCAAACGTTCCTTTGCTACCCCA
  CATGTCAATTCCAAATCACCCTGGTGGAATGGGTATCACTGTTTTTGGCAACAAATCTCAAAAACCGTAC
  AAAATAGACTCAAAACAATCGTCTTTGCTTGGGGACTCCCATATCCATGGGCACACCGAATCTATGATTT
  CTGCAGAAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224803 representing NM_013845
  Red=Cloning site Green=Tags(s)
  
  MHRPRRRGTRPPPLALLAALLLAARGADAQETELSVSAELVPTSSWNTSSEIDKGSYLTLDEPMNNITTS
  LGQTAELHCKVSGNPPPSIRWFKNDAPVVQEPRRISFRATNYGSRLRIRNLDTTDTGYFQCVATNGKKVV
  STTGVLFVKFGPPPTASPGSSDEYEEDGFCQPYRGIACARFIGNRTVYMESLHMQGEIENQITAAFTMIG
  TSSHLSDKCSQFAIPSLCHYAFPYCDETSSVPKPRDLCRDECEVLENVLCQTEYIFARSNPMILMRLKLP
  NCEDLPQPESPEAANCIRIGIPMADPINKNHKCYNSTGVDYRGTVSVTKSGRQCQPWNSQYPHTHSFTAL
  RFPELNGGHSYCRNPGNQKEAPWCFTLDENFKSDLCDIPACDSKDSKEKNKMEILYILVPSVAIPLAIAF
  LFFFICVCRNNQKSSSPPVQRQPKPVRGQNVEMSMLNAYKPKSKAKELPLSAVRFMEELGECTFGKIYKG
  HLYLPGMDHAQLVAIKTLKDYNNPQQWTEFQQEASLMAELHHPNIVCLLGAVTQEQPVCMLFEYMNQGDL
  HEFLIMRSPHSDVGCSSDEDGTVKSSLDHGDFLHIAIQIAAGMEYLSSHFFVHKDLAARNILIGEQLHVK
  ISDLGLSREIYSADYYRVQSKSSLPIRWMPPEAIMYGKFSSDSDIWSFGVVLWEIFSFGLQPYYGFSNQE
  VIEMVRKRQLLPCSEDCPPRMYSLMTECWNEIPSRRPRFKDIHVRLRSWEGLSSHTSSTTPSGGNATTQT
  TSLSASPVSNLSNPRFPNYMFPSQGITPQGQIAGFIGPAIPQNQRFIPINGYPIPPGYAAFPAAHYQPAG
  PPRVIQHCPPPKSRSPSSASGSTSTGHVASLPSSGSNQEANVPLLPHMSIPNHPGGMGITVFGNKSQKPY
  KIDSKQSSLLGDSHIHGHTESMISAEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013845
• ORF Size: 2811 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013845.5
• RefSeq Size: 3542 bp
• RefSeq ORF: 2814 bp
• Locus ID: 26563
• UniProt ID: Q9Z139
• Cytogenetics: 4 45.71 cM
• MW: 104.5 kDa
• Gene Summary: This gene encodes a receptor tyrosine kinase that has been implicated in nervous system development, specifically in the maintenance of neural progenitor cell fate, neurite extension and synapse formation. The encoded protein, likely a pseudokinase that lacks catalytic activity, may also regulate adipogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]