Myo18a (NM_011586) Mouse Tagged ORF Clone

CAT#: MR224798

Myo18a (Myc-DDK-tagged) - Mouse myosin XVIIIA (Myo18a)

Product Images

Circular map for MR224798

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Myo18a
• Synonyms: MAJN; MyoPDZ; MysPDZ; SP-R210
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224798 representing NM_011586
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAACCTCATGAAGAAAGATAAGGACAAAGATGGCGGGCGGAAGGAGAAGAAGGAAAAAAAGGAGA
  AGAAGGAGAGGATGTCTGCGGCAGAACTGCGGAGCCTGGAAGAGATGAGCATGCGCCGTGGCTTTTTCAA
  CTTGAACCGGTCCTCCAAGCGGGAATCTAAGACGCGCCTGGAAATCTCCAACCCCATCCCTATCAAGGTG
  GCCAGTGGCTCTGACCTGCACCTGACGGACATTGACTCTGACAGCAATCGGGGTAGCATCATCCTGGACT
  CAGGACACCTAAGCACAGCCAGCTCCAGTGATGACCTCAAGGGTGAAGAAGGCAGCTTCCGGGGCTCTGT
  GCTGCAGCGGGCAGCCAAGTTCGGTTCACTGGCCAAGCAGAACTCTCAGATGATTGTCAAACGTTTTTCC
  TTCTCTCAACGGAGCCGGGATGAGAGTGCCTCGGAGACCTCAACCCCCTCAGAGCACTCTGCAGCCCCAT
  CACCTCAGGTAGAGGTGCGGACGCTTGAGGGACAGCTAATGCAGCATCCTGGGCTAGGCATTCCTCGACC
  AGGACCACGGTCTCGAGTCCCTGAGCTGGTGACTAAAAGATTCCCAGCTGATCTACGTCTTCCCGCTCTG
  GTGCCACCACCACCTCCTGCTCTCCGGGAGCTGGAGTTACAACGAAGACCCACAGGAGACTTTGGCTTCT
  CACTGCGGCGCACAACCATGCTGGATCGGGCCCCTGAGGGTCAGGCTTATCGACGGGTGGTTCACTTTGC
  TGAGCCAGGGGCAGGCACCAAGGACCTGGCCTTGGGGCTGGTACCAGGAGATCGACTGGTGGAGATTAAT
  GGACAGAATGTGGAGAATAAATCCAGGGATGAAATTGTGGAGATGATCCGGCAGTCTGGGGACAGTGTGC
  GGCTCAAGGTACAGCCCATCCCAGAGCTTAGCGAGCTGAGCCGGAGCTGGCTTCGGACTGGCGAGGGACA
  CCGCAGGGAGCCGGCTGATGCCAAGACGGAAGAGCAGATTGCCGCTGAAGAGGCCTGGTATGAGACGGAG
  AAGGTGTGGCTGGTCCATAGAGATGGATTCTCCTTAGCCAGCCAGCTCAAGTCTGAGGAGCTCAGCCTAC
  CCGAGGGGAAGGCGCGTGTGAAGCTGGACCATGACGGAGCCATCCTGGATGTGGATGAAGATGATATAGA
  GAAGGCTAATGCTCCTTCATGTGACCGTCTAGAAGATCTGGCCTCACTGGTATACCTCAACGAGTCTAGC
  GTCCTGCATACACTGCGCCAGCGCTATGGGGCTAGCCTGCTACACACCTATGCTGGCCCCAGCCTGCTTG
  TCCTTAGCACCCGAGGGGCTCCTGCTGTGTATTCAGAGAAGGTGATGCACATGTTCAAGGGGTGTCGGCG
  GGAGGACATGGCACCCCACATCTACGCTGTAGCCCAGACTGCATATAGGGCGATGTTGATGAGTCGTCAG
  GACCAGTCCATTGTCCTTCTGGGTAGTAGTGGCAGTGGCAAGACTACCAGCTTTCAGCATCTGGTGCAGT
  ATCTGGCTACCATCGCGGGTACCAGTGGGACCAAGGTGTTCTCAGTGGAGAAGTGGCAGGCTCTGTCCAC
  CCTCCTGGAAGCCTTTGGGAACAGCCCCACCATCATGAATGGCAGTGCCACCCGCTTCTCCCAGATCCTC
  TCCCTGGACTTTGACCAAGCTGGCCAGGTGGCTTCGGCCTCCATCCAGACAATGCTCCTGGAGAAGCTGC
  GTGTGGCCCGGCGCCCAGCCAGTGAGGCTACTTTCAATGTCTTCTACTACTTGCTGGCCTGTGGGGATGC
  CACCCTCAGGACAGAGCTCCACCTGAACCACTTGGCAGAGAACAATGTGTTTGGAATTGTGCCACTGTCC
  AAGCCTGAGGAGAAGCAGAAGGCTGCACAGCAGTTCAGTAAGCTCCAGGCTGCCATGAAGGTGCTGGCCA
  TCTCCCCCGAGGAACAGAAGACCTGCTGGCTCATCCTGGCTTCCATCTACCACCTGGGGGCTGCTGGAGC
  TACCAAAGAAGCTGCTGAAGCTGGACGAAAGCAGTTTGCCCGCCACGAATGGGCCCAGAAGGCAGCATAC
  CTGCTGGGCTGCAGCTTGGAAGAGCTGTCCTCGGCTATCTTCAAACACCAGCTAAAAGGAGGCACCCTGC
  AACGGTCCACCTCCTTCCGTCAGGGCCCTGAGGAGAGCGGCCTGGGAGAGGGCACCAAACTGAGTGCTCT
  GGAGTGCTTAGAGGGTATGGCGTCTGGCCTCTACAGTGAGCTCTTTACTCTCCTCATCTCCCTGGTAAAC
  AGGGCTCTCAAGTCCAGCCAGCACTCACTCTGTTCCATGATGATCGTGGACACACCAGGCTTCCAGAACC
  CTGAATGGGGCGGCTCGGCCCGTGGAGCCTCCTTCGAGGAGCTGTGCCACAACTATGCCCAGGACAGGCT
  GCAGAGGCTGTTCCACGAGCGCACCTTCCTCCAGGAGTTGGAGAGATACAAGGAGGATAACATAGAACTG
  GCGTTTGATGACTTGGAGCCAGTTGCAGATGACTCCGTGGCTGCTGTGGACCAGGCTTCCCACCTGGTTC
  GCTCACTGGCCCATGCCGATGAGGCAAGAGGGCTACTGTGGCTCCTGGAAGAGGAGGCACTGGTGCCTGG
  TGCGACAGAGGATGCCCTCCTTGATCGTCTTTTCTCCTATTATGGCCCCCAGGAGGGTGACAAAAAAGGC
  CAGAGTCCTCTTCTGCGAAGTAGCAAACCTCGGCACTTTCTCCTGGGCCACAGCCACGGTACCAACTGGG
  TGGAGTACAACGTGGCTGGCTGGCTGAATTACACCAAGCAGAACCCAGCCACCCAGAATGCACCCCGGCT
  TCTGCAGGACTCCCAGAAAAAGATCATCAGTAACCTGTTTCTGGGCCGGGCGGGCAGTGCCACGGTGCTC
  TCAGGCTCGATTGCAGGCCTGGAAGGTGGCTCCCAGCTGGCCTTGCGCCGGGCAACCAGCATGAGGAAAA
  CCTTCACCACCGGCATGGCCGCTGTCAAGAAGAAGTCGCTGTGCATACAGATTAAGCTGCAAGTAGATGC
  CCTCATTGATACCATCAAGAGGTCCAAGATGCATTTCGTGCACTGCTTCCTTCCTGTGGCCGAGGGCTGG
  CCTGGGGAGCCCCGGTCTGCCTCTTCCCGCAGAGTCAGCAGCAGCAGTGAGCTGGACCTGCCCCCGGGAG
  ACCCCTGTGAGGCTGGGCTGCTACAACTGGATGTGTCCCTGCTCCGAGCCCAGCTCCGGGGATCCCGCCT
  GCTTGATGCGATGCGCATGTACCGCCAAGGTTACCCAGACCACATGGTGTTTTCTGAGTTCCGCCGGCGC
  TTTGATGTCCTGGCTCCACACCTGACCAAGAAACATGGTCGTAACTACATCGTGGTGGATGAGAAGCGGG
  CTGTGGAAGAGCTGCTGGAGTCCCTGGACCTGGAAAAGAGCAGCTGCTGTCTGGGCCTAAGCCGGGTGTT
  CTTCCGGGCAGGCACGTTGGCACGGTTGGAAGAACAACGAGACGAGCAAACCAGCAGGCACCTGACCCTG
  TTCCAGGCAGCCTGCCGGGGCTACCTTGCCCGCCAGCACTTCAAGAAGAGAAAGATCCAGGACCTGGCCA
  TTCGCTGTGTGCAGAAGAACATCAAGAAGAACAAGGGGGTGAAGGACTGGCCCTGGTGGAAGCTCTTCAC
  CACCGTGCGGCCCCTCATCCAAGTTCAGCTGTCAGAGGAGCAGATCCGAAACAAAGACGAGGAGATCCAG
  CAGCTGCGCAGCAAGCTTGAGAAGGTGGAGAAGGAGAGGAACGAGCTCCGACTCAGCAGTGACCGGCTAG
  AGACCCGGATCTCAGAACTGACATCGGAGCTGACTGATGAACGCAACACGGGAGAGTCCGCCTCCCAGCT
  GCTGGACGCGGAGACAGCCGAGAGGCTCCGGACCGAGAAGGAGATGAAAGAGCTACAGACCCAGTATGAC
  GCACTGAAGAAGCAGATGGAGGTGATGGAGATGGAAGTGATGGAAGCCCGGCTCATTCGGGCAGCCGAGA
  TCAACGGGGAGGTGGATGATGATGATGCAGGGGGAGAGTGGAGGCTGAAGTACGAGCGAGCTGTTCGGGA
  GGTGGACTTCACCAAGAAGCGGCTCCAGCAGGAGCTGGAAGACAAGATGGAGGTGGAACAGCAGAGCAGG
  AGGCAGCTGGAGAGGCGGCTTGGGGACCTGCAAGCAGATAGTGATGAGAGTCAACGGGCACTGCAGCAGC
  TCAAGAAGAAGTGCCAAAGGCTCACAGCTGAGCTGCAGGACACCAAGCTGCACCTGGAGGGCCAGCAGGT
  CCGAAACCACGAGCTGGAGAAGAAGCAGAGGAGGTTCGACAGTGAGCTTTCCCAGGCCCACGAGGAGACG
  CAGCGGGAGAAGCTGCAGAGGGAGAAACTCCAGCGGGAGAAGGACATGCTCCTGGCTGAGGCTTTCAGCT
  TGAAGCAGCAAATGGAGGAAAAAGACTTGGACATTGCGGGGTTTACCCAGAAGGTGGTTTCCTTGGAGGC
  TGAGCTTCAGGACATTTCTTCTCAAGAGTCTAAGGATGAGGCTTCTCTGGCCAAGGTCAAGAAGCAGCTC
  CGGGACTTGGAGGCCAAGGTCAAGGACCAGGAAGAGGAGCTGGATGAGCAGGCTGGGAGTATACAGATGC
  TGGAGCAGGCCAAGCTGCGTCTGGAGATGGAGATGGAGAGAATGAGACAGACCCATTCCAAGGAGATGGA
  GAGTCGGGATGAGGAGGTGGAAGAGGCCCGGCAGTCATGTCAGAAGAAGTTAAAACAGATGGAAGTGCAG
  CTCGAGGAGGAGTATGAAGACAAGCAGAAGGCGCTGCGGGAGAAACGGGAGCTGGAGAGCAAGCTCTCCA
  CGCTCAGTGACCAGGTGAACCAGCGGGACTTTGAATCAGAGAAGCGGCTACGGAAAGACCTGAAGCGCAC
  CAAAGCGCTGCTGGCAGATGCCCAGATCATGCTGGACCACTTAAAGAACAATGCCCCGAGCAAGAGGGAG
  ATTGCCCAGCTGAAGAACCAGCTGGAAGAGTCGGAATTCACCTGTGCAGCAGCTGTAAAGGCACGGAAAG
  CGATGGAGGTGGAGATGGAAGACCTGCACCTGCAGATTGATGACATCGCCAAAGCCAAGACAGCGCTGGA
  GGAGCAACTGAGTCGACTTCAGCGTGAGAAAAACGAGATTCAAAACCGGCTGGAAGAGGATCAGGAGGAC
  ATGAATGAGCTGATGAAGAAGCACAAGGCAGCTGTGGCCCAGGCCTCCCGGGACATGGCACAGATGAACG
  ATCTCCAGGCTCAGATTGAAGAGTCCAACAAGGAGAAGCAAGAGCTACAGGAGAAGCTACAAGCTCTGCA
  GAGCCAGGTGGAGTTCCTGGAGCAGTCCATGGTGGACAAGTCCCTCGTCAGCAGGCAGGAAGCGAAGATC
  AGGGAGCTGGAGACACGCCTGGAGTTCGAAAAGACCCAAGTGAAGCGCCTGGAGAACCTGGCCAGTCGGC
  TCAAAGAAACCATGGAGAAGCTGACTGAGGAACGGGACCAGCGCGCCGCAGCTGAGAACCGTGAGAAGGA
  GCAGAACAAGAGGCTCCAGCGACAGCTTCGTGACACCAAGGAGGAGATGAGCGAGCTTGCCAGGAAGGAA
  GCCGAGGCTAGCCGCAAGAAGCATGAACTGGAGATGGACCTGGAGAGCCTGGAAGCTGCTAACCAAAGCC
  TGCAAGCCGACCTAAAGCTGGCGTTCAAACGCATTGGGGACTTGCAAGCTGCCATTGAGGATGAGATGGA
  AAGTGACGAGAACGAGGACCTCATCAACAGTGAGGGGGACTCAGATGTGGACTCAGAGCTGGAGGACCGG
  GTCGACGGGGTCAAGTCCTGGTTGTCAAAGAACAAGGGACCTTCTAAGGCACCTTCTGACGATGGCAGCT
  TGAAGAGTTCCAGCCCAACCAGCCACTGGAAGCCACTCGCCCCTGACCCATCGGATGATGAGCATGATCC
  TGTGGACAGCATCTCCAGACCCCGGTTCTCCCACAGCTATCTGAGTGACAGCGACACAGAGGCCAAGCTG
  ACAGAGACCAGTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224798 representing NM_011586
  Red=Cloning site Green=Tags(s)
  
  MFNLMKKDKDKDGGRKEKKEKKEKKERMSAAELRSLEEMSMRRGFFNLNRSSKRESKTRLEISNPIPIKV
  ASGSDLHLTDIDSDSNRGSIILDSGHLSTASSSDDLKGEEGSFRGSVLQRAAKFGSLAKQNSQMIVKRFS
  FSQRSRDESASETSTPSEHSAAPSPQVEVRTLEGQLMQHPGLGIPRPGPRSRVPELVTKRFPADLRLPAL
  VPPPPPALRELELQRRPTGDFGFSLRRTTMLDRAPEGQAYRRVVHFAEPGAGTKDLALGLVPGDRLVEIN
  GQNVENKSRDEIVEMIRQSGDSVRLKVQPIPELSELSRSWLRTGEGHRREPADAKTEEQIAAEEAWYETE
  KVWLVHRDGFSLASQLKSEELSLPEGKARVKLDHDGAILDVDEDDIEKANAPSCDRLEDLASLVYLNESS
  VLHTLRQRYGASLLHTYAGPSLLVLSTRGAPAVYSEKVMHMFKGCRREDMAPHIYAVAQTAYRAMLMSRQ
  DQSIVLLGSSGSGKTTSFQHLVQYLATIAGTSGTKVFSVEKWQALSTLLEAFGNSPTIMNGSATRFSQIL
  SLDFDQAGQVASASIQTMLLEKLRVARRPASEATFNVFYYLLACGDATLRTELHLNHLAENNVFGIVPLS
  KPEEKQKAAQQFSKLQAAMKVLAISPEEQKTCWLILASIYHLGAAGATKEAAEAGRKQFARHEWAQKAAY
  LLGCSLEELSSAIFKHQLKGGTLQRSTSFRQGPEESGLGEGTKLSALECLEGMASGLYSELFTLLISLVN
  RALKSSQHSLCSMMIVDTPGFQNPEWGGSARGASFEELCHNYAQDRLQRLFHERTFLQELERYKEDNIEL
  AFDDLEPVADDSVAAVDQASHLVRSLAHADEARGLLWLLEEEALVPGATEDALLDRLFSYYGPQEGDKKG
  QSPLLRSSKPRHFLLGHSHGTNWVEYNVAGWLNYTKQNPATQNAPRLLQDSQKKIISNLFLGRAGSATVL
  SGSIAGLEGGSQLALRRATSMRKTFTTGMAAVKKKSLCIQIKLQVDALIDTIKRSKMHFVHCFLPVAEGW
  PGEPRSASSRRVSSSSELDLPPGDPCEAGLLQLDVSLLRAQLRGSRLLDAMRMYRQGYPDHMVFSEFRRR
  FDVLAPHLTKKHGRNYIVVDEKRAVEELLESLDLEKSSCCLGLSRVFFRAGTLARLEEQRDEQTSRHLTL
  FQAACRGYLARQHFKKRKIQDLAIRCVQKNIKKNKGVKDWPWWKLFTTVRPLIQVQLSEEQIRNKDEEIQ
  QLRSKLEKVEKERNELRLSSDRLETRISELTSELTDERNTGESASQLLDAETAERLRTEKEMKELQTQYD
  ALKKQMEVMEMEVMEARLIRAAEINGEVDDDDAGGEWRLKYERAVREVDFTKKRLQQELEDKMEVEQQSR
  RQLERRLGDLQADSDESQRALQQLKKKCQRLTAELQDTKLHLEGQQVRNHELEKKQRRFDSELSQAHEET
  QREKLQREKLQREKDMLLAEAFSLKQQMEEKDLDIAGFTQKVVSLEAELQDISSQESKDEASLAKVKKQL
  RDLEAKVKDQEEELDEQAGSIQMLEQAKLRLEMEMERMRQTHSKEMESRDEEVEEARQSCQKKLKQMEVQ
  LEEEYEDKQKALREKRELESKLSTLSDQVNQRDFESEKRLRKDLKRTKALLADAQIMLDHLKNNAPSKRE
  IAQLKNQLEESEFTCAAAVKARKAMEVEMEDLHLQIDDIAKAKTALEEQLSRLQREKNEIQNRLEEDQED
  MNELMKKHKAAVAQASRDMAQMNDLQAQIEESNKEKQELQEKLQALQSQVEFLEQSMVDKSLVSRQEAKI
  RELETRLEFEKTQVKRLENLASRLKETMEKLTEERDQRAAAENREKEQNKRLQRQLRDTKEEMSELARKE
  AEASRKKHELEMDLESLEAANQSLQADLKLAFKRIGDLQAAIEDEMESDENEDLINSEGDSDVDSELEDR
  VDGVKSWLSKNKGPSKAPSDDGSLKSSSPTSHWKPLAPDPSDDEHDPVDSISRPRFSHSYLSDSDTEAKL
  TETSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011586
• ORF Size: 6105 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011586.3, NP_035716.1
• RefSeq Size: 7360 bp
• RefSeq ORF: 6108 bp
• Locus ID: 360013
• UniProt ID: Q9JMH9
• Cytogenetics: 11 B5
• MW: 230.9 kDa
• Gene Summary: May link Golgi membranes to the cytoskeleton and participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus (PubMed:19837035). Alternatively, in concert with LURAP1 and CDC42BPA/CDC42BPB, has been involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (By similarity). May be involved in the maintenance of the stromal cell architectures required for cell to cell contact (PubMed:10733906). Regulates trafficking, expression, and activation of innate immune receptors on macrophages. Plays a role to suppress inflammatory responsiveness of macrophages via a mechanism that modulates CD14 trafficking (PubMed:25965346). Acts as a receptor of surfactant-associated protein A (SFTPA1/SP-A) and plays an important role in internalization and clearance of SFTPA1-opsonized S.aureus by alveolar macrophages (PubMed:21123169). Strongly enhances natural killer cell cytotoxicity (By similarity).[UniProtKB/Swiss-Prot Function]