Adamts17 (NM_001033877) Mouse Tagged ORF Clone

CAT#: MR224790

Adamts17 (Myc-DDK-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 17 (Adamts17)

Product Images

Circular map for MR224790

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adamts17
• Synonyms: AU023434
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224790 representing NM_001033877
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGACGGCACCCTGCTGCCCCCTCTCGTCCTGTCCTTGCTGCTGCTGGTGTGGGGGCTGGACCCGG
  GCACAGCTGTCGGCGACGCGGCGGCCGACGTGGAGGTGGTGCTCCCGAGGCGGCTGCGCCCCGAGGACGT
  GCACCTGCAGTTGCTGCCGGGAGCAGCGGGGCTCCGGCGGTGGCGGCGACCGCGAGCATCTCCAGGTGGC
  CATCGAGCAGGGCAAGGAGAGCGCGCCCTGCTACTGCACCTGCCGGCCTTTGGGCGCGACCTATACCTGC
  AGCTGCATCGCGACCTGCGTTTCCTGTCCCCAGGCTTCGAGGTGGAGGAGGCGGGTGTCTCGGGGCGCAG
  CGGACATCCTGCCGAGCTGTGCTTCTACTCCGGACGAGTCCTGGGCCACCCGGGCTCCCTCGTCTCCCTC
  AGCGCCTGCGGCGCCGCCGGCGGCCTGGTTGGCCTCATCCAGCTTGAGCAGGAGCAAGTTTTCATCCAGC
  CCCTCAACTCCTCAGGGGGCCCATTCATTGGAAGAGAGCATCTGATCAGACGCAAATGGGCCTCCACCCC
  CAGCCCTGCTACGGAGGTGGAGGGACCGAGGCAGCGCTGCAAAGTAGTGACAGAAAAGAAGCCCAGGAAG
  GACCATCTGTCTCGGGACTGGAGGGAAAGGAGGAATGCCATCCGGCTCACCAACGAACACACAGTGGAGA
  CCCTGGTGGTGGCCGATGCCGACATGGTGCAGTACCACGGGGCAGAGGCTGCACAAAGATTCATCCTCAC
  TGTCATGAACATGGTATACAACATGTTTCAGCACAGGAGCCTTGGGATTAAAATTAACATTCAAGTCACC
  AAGCTGGTCCTTCTGAGACAACGCCCCGCCAAGTTATCCATTGGGCACCATGGTGAGCGGTCCCTGGAGA
  GCTTCTGTCACTGGCAGAATGAGGAGTATGGAGGAGCACGCTACCTTGGCAATAACCAGGTGCCAGGAGG
  CAAGGATGATACACCTCCTGTGGATGCTGCTGTATTTGTGACCAGGACAGATTTCTGCGTTCACAAAGAT
  GAACCTTGCGACACTGTTGGAATTGCTTACTTAGGAGGCGTGTGCAGTGCTAAGAGGAAATGTGTGCTCG
  CCGAAGACAATGGTCTCAATTTGGCCTTTACCATCGCACATGAACTGGGCCACAACCTGGGGATGAACCA
  TGATGACGACCACTCATCTTGTGCTGGCCGGTCGCACATCATGTCCGGAGAGTGGGTAAAAGGACGGAAT
  CCCAGCGACCTCTCTTGGTCATCCTGTAGTCGAGATGACCTTGAGAACTTTCTCAAATCCAAAGTCAGTA
  CATGTTTACTGATCACTGATCCCAGGAGCCAGCATGCCCTGCGCCTTCCACACAAGCTGCCAGGCATGCA
  CTACAGCGCCAACGAGCAGTGCCAGATTCTGTTTGGCACCAATGCTACCTTCTGCAAAAACATGGAGCAT
  TTGATGTGTGCTGGGCTGTGGTGCCTGGTAGAGGGAGACACATCCTGCAAGACCAAGCTGGACCCTCCCC
  TGGATGGCACTGAATGTGGAGCAGACAAGTGGTGCCGGGCTGGAGAGTGTGTGAGCAAGACGCCCATCCC
  AGAGCACGTGGACGGGGACTGGAGTCTGTGGAGCGCCTGGAGCATGTGCAGCCGAACCTGTGGTACAGGA
  GCGCGATTCCGGCAGAGGAAATGTGACAACCCCCCCCCTGGACCTGGTGGCACACACTGCCAGGGTGCCA
  GCGTAGAACATGCTGCCTGTGAGAAGCTGCCCTGCCCCAAGGGTGTGCCCAGCTTCCGGGACCAGCAGTG
  CCAAGCCCATGACCGACTGAGCAGCAAGAAGAGAGGCCTGTTGACAGCAGTGGTGGTTGATGATAAACCC
  TGTGAACTCTACTGTTCACCCCTCGGGAAGGAGTCCCCGCTGCTGGTGGCTGACAGGGTCCTGGATGGCA
  CACCCTGTGGACCCTACGAGGCTGACCTCTGCGTGTATGGCAGATGCCAGAAAATTGGATGTGACGGCAT
  CATCGGATCTGCGGCTAAAGAAGATAGGTGTGGTGTCTGCAGCGGGGATGGCAAGACCTGCCGTGTGGTG
  AAGGGTGACTTCAGCCACTCCCGGGGGACAGGTTATATCGAAGCGGTTGTCATTCCGGTTGGAGCTCGGC
  GGATCCGTGTGGTGGAAGACAAACCTGCTCACAGCTTCCTGGCTCTCAAAGATTCCAGTAAGAGATCCAT
  CAACAGCGACTGGAAGATAGAACTCCCTGGAGAGTTCCAGATTGCAGGTACAACCGTCCGCTACGTGAGG
  AGAGGCTTGTGGGAGAAGTTCTCAGCCAAGGGACCCACCACAGTGCCGCTGCATCTGATGGTGCTGTTAT
  TTCATGACCAGAATTACGGAATTCATTATGAGTACACCATACCTGTAAACCAGAGTTCAGAAAACCAGAG
  TGAGCCCGCCAAGCCACAAGATGCTCTGTTCCTCTGGACCCACAGCGGCTGGGAAGGGTGCAGTGTGCAG
  TGTGGAGGAGGAGAACGCAGGACTATCGTATCATGCACACGGATTGTTAACAAGACTACAACCCTGGTGA
  ATGACAGTGACTGCCCCCAAGCAAGCCGCCCTGAGCCCCAGGTCCGACGGTGTAACTCTCACCCATGCCA
  GTCCCGGTGGGTGACTGGTCAGTGGAGCCCCTGCTCAGCGACCTGTGAGAAGGGCGTGCAGCATCGGGAG
  GTGACTTGCGTGTACCAGCTCCAGAACGGCACACACGTCACTACTCGGCCCATCTACTGCTCAGAACCCC
  GGCCAACACCGGTACAGAGCTGTGAAGGCCAGGACTGCCTGTCTATCTGGGAGGCTTCAGAGTGGTCAGA
  GTGCTCTGCCAACTGCGGCAAAGGGATCCAAAAACGGACTGTGACATGTACCAACTCCCAAGGGAAATGT
  GACGCGTCCACAAGGCCGAAAGCAGAGGAGGAGTGCGAGGACTACTCTGGCTGCTATGAGTGGAAAACGG
  GGGACTGGTCTAAGTGCTCGTCCACCTGTGGGAAGGGGCTTCAGTCAAGAGTGGTCCAGTGTATGCACAA
  GCTCACTGGACGCCATGGCAGTGAGTGCCCGACTCTCTCAAAGCCAGCAGCCTACAGACAGTGCCACCAG
  GAGGTCTGCAACGAGAAGATCAATGTGAACACCATCACCTCCCCTCGCCTTGCCGCTCTGACCTACAAAT
  GCACTCGGGACCAGTGGACGGTGTACTGCCGGGTCATCCGAGAGAAGAACCTCTGCCAGGATATGCGATG
  GTACCAGCGCTGCTGCCAGACCTGTAGGGACTTCTACGCAGATAAGATGCGCCAGCCGCCCTCTCCACCA
  AGCTCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224790 representing NM_001033877
  Red=Cloning site Green=Tags(s)
  
  MCDGTLLPPLVLSLLLLVWGLDPGTAVGDAAADVEVVLPRRLRPEDVHLQLLPGAAGLRRWRRPRASPGG
  HRAGQGERALLLHLPAFGRDLYLQLHRDLRFLSPGFEVEEAGVSGRSGHPAELCFYSGRVLGHPGSLVSL
  SACGAAGGLVGLIQLEQEQVFIQPLNSSGGPFIGREHLIRRKWASTPSPATEVEGPRQRCKVVTEKKPRK
  DHLSRDWRERRNAIRLTNEHTVETLVVADADMVQYHGAEAAQRFILTVMNMVYNMFQHRSLGIKINIQVT
  KLVLLRQRPAKLSIGHHGERSLESFCHWQNEEYGGARYLGNNQVPGGKDDTPPVDAAVFVTRTDFCVHKD
  EPCDTVGIAYLGGVCSAKRKCVLAEDNGLNLAFTIAHELGHNLGMNHDDDHSSCAGRSHIMSGEWVKGRN
  PSDLSWSSCSRDDLENFLKSKVSTCLLITDPRSQHALRLPHKLPGMHYSANEQCQILFGTNATFCKNMEH
  LMCAGLWCLVEGDTSCKTKLDPPLDGTECGADKWCRAGECVSKTPIPEHVDGDWSLWSAWSMCSRTCGTG
  ARFRQRKCDNPPPGPGGTHCQGASVEHAACEKLPCPKGVPSFRDQQCQAHDRLSSKKRGLLTAVVVDDKP
  CELYCSPLGKESPLLVADRVLDGTPCGPYEADLCVYGRCQKIGCDGIIGSAAKEDRCGVCSGDGKTCRVV
  KGDFSHSRGTGYIEAVVIPVGARRIRVVEDKPAHSFLALKDSSKRSINSDWKIELPGEFQIAGTTVRYVR
  RGLWEKFSAKGPTTVPLHLMVLLFHDQNYGIHYEYTIPVNQSSENQSEPAKPQDALFLWTHSGWEGCSVQ
  CGGGERRTIVSCTRIVNKTTTLVNDSDCPQASRPEPQVRRCNSHPCQSRWVTGQWSPCSATCEKGVQHRE
  VTCVYQLQNGTHVTTRPIYCSEPRPTPVQSCEGQDCLSIWEASEWSECSANCGKGIQKRTVTCTNSQGKC
  DASTRPKAEEECEDYSGCYEWKTGDWSKCSSTCGKGLQSRVVQCMHKLTGRHGSECPTLSKPAAYRQCHQ
  EVCNEKINVNTITSPRLAALTYKCTRDQWTVYCRVIREKNLCQDMRWYQRCCQTCRDFYADKMRQPPSPP
  SS
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033877
• ORF Size: 3366 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033877.4, NP_001029049.2
• RefSeq Size: 6043 bp
• RefSeq ORF: 3369 bp
• Locus ID: 233332
• Cytogenetics: 7 C
• MW: 124.2 kDa