Amotl1 (NM_001081395) Mouse Tagged ORF Clone

CAT#: MR224679

Amotl1 (Myc-DDK-tagged) - Mouse angiomotin-like 1 (Amotl1)

Product Images

Circular map for MR224679

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Amotl1
• Synonyms: 2310010G08Rik; 2310067L22Rik; 4932416D09Rik; JEAP; mFLJ00155
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224679 representing NM_001081395
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGAGGGCAAAGTCGCGCCGGGGACCTTGCGAGCCTGTGCTTAGAGCTCCTCCTCCCATCTGCTACA
  GCCCCAGCAGTCCTGTGCAGATTCTAGAAGACCCCGCCTACTTCTACCCAGACTTGCAGCTCTATTCTGG
  GAGGCATGAAGCATCTACTTTGACAGTGGAGGCAAGTGGAGGCCTAAGGGGGAAAAGCGTAGAGGACCCT
  CTCTCCAGCTTCCACTCCCCGAACTTCCTGAGGACGCCCGAGGTGGAAATGAGAGGTTCTGAGGATGTAG
  CCTCTGGAAGAGTGTTACAGCGGCTGATCCAGGAACAACTGCGCTATGGCACCCCAACTGAGAACATGAA
  CCTGCTGGCCATTCAGCACCAGGCCACAGGGAGTGCAGGGCCAGCCCACGCCACCACCAACTTTTCTTCC
  ACGGAAACCCTCACTCAAGAAGATCCACAAATGGTCTATCAGTCGGCCCGCCAAGAACCGCAGGGTCAAG
  AGCATCAGGGAGACAATACGGTGATGGAGAAGCAGGTCCGGTCCACACAGCCTCAGCAGAACAACGAGGA
  GCTCCCCACGTATGAGGAAGCCAAGGCCCAGTCCCAGTTCTTCAGGGGACAGCAGCAGCAGCAGCAACAG
  CAGCAGCAGCAACAGCAGCAGCAACAGCAGCAGGGACAGGGGCCCCTTAGCCACACTTATTACATGGCCG
  GAGGTACCAGTCAGAAGTCCCGCACTGAGGGGAGGCCCACAGTGAACCGGGCCAACAGTGGACAGGCGCA
  TAAGGATGAGGCCCTGAAAGAACTAAAGCAGGGCCATGTCCGGTCCCTCAGTGAGAGGATCATGCAGCTG
  TCACTGGAGAGGAATGGGGCTAAGCAACACCTCCCCAGCTCTGGAAATGGAAAGAGCTTCAAAGCTGGAG
  GAGAGCCATCCCCAGCTCAGCCTGTCTGTAAAGCACTGGACCCTCGTGGCCCTCCACCTGAATACCCCTT
  CAAGACCAAGCCAATGAAATCCCCAGTCAGCAAGAACCAAGATCACGGTCTTTACTACAATGACCAGCAC
  CCTGGGGTACTCCATGAGATGGTCAAACCTTACCCAGCACCTCAGCCTGCGAGAACAGAAGTGGCCGTCC
  TGAGGTATCAGCCACCCCCGGAGTATGGCGTCACCAGCCGGCCCTGCCAGCTGCCTTTTCCATCTACGGT
  GCAGCAGCATAGCCCCATGTCCTCTCAGACCTCCTCCATCGGTGGTACTCTGCACTCCGTCTCCCTGCCT
  CTTCCACTTCCCATAAGCCTGGCGGCTTCACAGCCCCTACCAGCCTCCCCCAACCAGCAGCTTGGACCGG
  ATGCCTTTGCGATTGTGGAGCGAGCCCAGCAAATGGTAGAGATCCTGACAGAGGAGAACCGTGTGCTTCA
  CCAGGAGCTTCAGGGCTGCTATGACAACGCTGACAAGCTCCACAAGTTTGAAAAAGAGCTGCAGAGTATT
  TCGGAGGCCTACGAGAGCCTGGTCAAGTCCACCACCAAGCGTGAGTCTCTGGACAAGGCAATGAGAACCA
  AGCTCGAAGGCGAGATAAGGAGACTTCATGACTTCAACAGAGATCTCCGAGATCGACTGGAGACAGCCAA
  CAGGCAGCTGTCCAGCAGGGAATACGATGGGCATGAAGACAAAGCTGCAGAGAGCCATTACGTGTCCCAG
  AACAAAGAATTCTTGAAGGAAAAGGAAAAGTTGGAAATGGAGTTGGCAGCAGTGCGTACGGCAAGTGAGG
  ACCATCGGAGGCACATCGAGATCCTGGACCAGGCTTTGAGCAATGCGCAGGCTAGAGTGATCAAACTGGA
  GGAAGAGTTACGAGAGAAGCAAGCCTATGTGGAAAAGGTGGAGAAGCTGCAGCAGGCCCTGACCCAGCTG
  CAGTCAGCGTGCGAGAAGCGAGGGCAGATGGAACGCAGGTTGCGGACCTGGCTGGAGAGGGAGCTAGACG
  CTCTGAGGACACAGCAGAAACATGGCACAGGCCCTCCAGTCAGTCTCCCAGAATGTAATGCTCCTGCCCT
  CATGGAGCTGGTGAGGGAGAAGGAGGAGCGGATCCTCGCCTTGGAGGCCGACATGACCAAGTGGGAGCAG
  AAGTACCTGGAAGAGAGCACCATCCGGCACTTTGCCATGAGCGCAGCTGCCGCTGCCACAGCCGAGAGGG
  ACACCACCATCAGCAACCACTCGAGGAATGGCAGCTATGGGGAGAGCTCCCTGGAGGCCCACATCTGGCC
  AGAGGAAGAAGAAGTGGTGCAGGCCAACAGGAGGTGTCAGGACATGGAGTACACTATTAAAAACCTCCAT
  GCCAAAATCATAGAGAAGGATGCCATGATAAAGGTCCTGCAGCAGCGATCCCGTAAGGATGCTGGGAAGA
  CGGACTCTGCCAGCCTGAGGCCTGCCCGCTCTGTCCCATCCATCGCTGCGGCCACTGGGACACATTCTCG
  CCAGACTTCTCTTACCAGCAGCCAGCTGACAGAAGAGAAAAAGGAAGAGAAGACGACCTGGAAAGGGAGT
  ATAGGATTCCTGCTGGGAAAGGAACACCAGGGACAGGCATCTGCCCCTCTGCTGCCGACCACACCTGCCT
  CTGCATTGTCCCTTCCGGCCTCTACCACATCGGCCAGCAGCACCCACGCCAAGACGGGCAGCAAGGACAG
  CAGCACACAGACCGACAAGAGCACGGAGCTCTTCTGGCCCAGCATGGCTTCCCTCCCCAGCCGTGGCAGG
  CTGAGCACAGCCCCTTCCAACAGCCCCATCCTAAAGCATCCAGCTGCCAAAGGAGCCGTGGAGAAGCAGG
  AGAACTCTCCTGGTCATGGGAAGGCATCGGAGCACAGAGGCCGAGTCAGCAACTTGCTGCACAAGCCTGA
  GTTCCCAGATGGCGAGATGATGGAAGTGCTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224679 representing NM_001081395
  Red=Cloning site Green=Tags(s)
  
  MRRAKSRRGPCEPVLRAPPPICYSPSSPVQILEDPAYFYPDLQLYSGRHEASTLTVEASGGLRGKSVEDP
  LSSFHSPNFLRTPEVEMRGSEDVASGRVLQRLIQEQLRYGTPTENMNLLAIQHQATGSAGPAHATTNFSS
  TETLTQEDPQMVYQSARQEPQGQEHQGDNTVMEKQVRSTQPQQNNEELPTYEEAKAQSQFFRGQQQQQQQ
  QQQQQQQQQQQGQGPLSHTYYMAGGTSQKSRTEGRPTVNRANSGQAHKDEALKELKQGHVRSLSERIMQL
  SLERNGAKQHLPSSGNGKSFKAGGEPSPAQPVCKALDPRGPPPEYPFKTKPMKSPVSKNQDHGLYYNDQH
  PGVLHEMVKPYPAPQPARTEVAVLRYQPPPEYGVTSRPCQLPFPSTVQQHSPMSSQTSSIGGTLHSVSLP
  LPLPISLAASQPLPASPNQQLGPDAFAIVERAQQMVEILTEENRVLHQELQGCYDNADKLHKFEKELQSI
  SEAYESLVKSTTKRESLDKAMRTKLEGEIRRLHDFNRDLRDRLETANRQLSSREYDGHEDKAAESHYVSQ
  NKEFLKEKEKLEMELAAVRTASEDHRRHIEILDQALSNAQARVIKLEEELREKQAYVEKVEKLQQALTQL
  QSACEKRGQMERRLRTWLERELDALRTQQKHGTGPPVSLPECNAPALMELVREKEERILALEADMTKWEQ
  KYLEESTIRHFAMSAAAAATAERDTTISNHSRNGSYGESSLEAHIWPEEEEVVQANRRCQDMEYTIKNLH
  AKIIEKDAMIKVLQQRSRKDAGKTDSASLRPARSVPSIAAATGTHSRQTSLTSSQLTEEKKEEKTTWKGS
  IGFLLGKEHQGQASAPLLPTTPASALSLPASTTSASSTHAKTGSKDSSTQTDKSTELFWPSMASLPSRGR
  LSTAPSNSPILKHPAAKGAVEKQENSPGHGKASEHRGRVSNLLHKPEFPDGEMMEVLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081395
• ORF Size: 2904 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081395.1, NP_001074864.1
• RefSeq Size: 8951 bp
• RefSeq ORF: 2907 bp
• Locus ID: 75723
• UniProt ID: Q9D4H4
• Cytogenetics: 9 A2
• MW: 108.4 kDa
• Gene Summary: Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus.[UniProtKB/Swiss-Prot Function]